Abundance and Community Structure of Sphingomonads in Leaf Residues and Nearby Bulk Soil

Murakami, Yuta; Otsuka, Shigeto; Senoo, Keishi

doi:10.1264/jsme2.me10114

Cited by 11 publications

(11 citation statements)

References 41 publications

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“…1B). While this group did not make it into our source-tracking analysis (members of the order Sphingomonadales were not an abundant group in the phyllosphere study used in our current meta-analysis [40]), this particular group is known from other studies to be a common inhabitant of leaves (23,36), supporting our hypothesis that leaves are an important source of bacteria in the atmosphere during the summer months. The nearby freshwater communities of the Great Lakes are another on May 12, 2018 by guest http://aem.asm.org/ possible source of bacteria in the atmosphere in the summer months.…”

Section: Resultssupporting

confidence: 65%

Sources of Bacteria in Outdoor Air across Cities in the Midwestern United States

Bowers

Sullivan

Costello

et al. 2011

Appl Environ Microbiol

247

207

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Bacteria are abundant in the atmosphere, where they often represent a major portion of the organic aerosols. Potential pathogens of plants and livestock are commonly dispersed through the atmosphere, and airborne bacteria can have important effects on human health as pathogens or triggers of allergic asthma and seasonal allergies. Despite their importance, the diversity and biogeography of airborne microorganisms remain poorly understood. We used high-throughput pyrosequencing to analyze bacterial communities present in the aerosol fraction containing fine particulate matter of <2.5 m from 96 near-surface atmospheric samples collected from cities throughout the midwestern United States and found that the communities are surprisingly diverse and strongly affected by the season. We also directly compared the airborne communities to those found in hundreds of samples representing potential source environments. We show that, in addition to the more predictable sources (soils and leaf surfaces), fecal material, most likely dog feces, often represents an unexpected source of bacteria in the atmosphere at more urbanized locations during the winter. Airborne bacteria are clearly an important, but understudied, component of air quality that needs to be better integrated into efforts to measure and model pollutants in the atmosphere.Scientists have long known that bacteria are ubiquitous in the atmosphere. Bacterial concentrations typically range from 10 4 to 10 6 cells m Ϫ3 (28), though concentrations may be far higher in proximity to point sources such as compost facilities, feedlots, and wastewater treatment facilities (1,24,41). Recent evidence suggests that, even in some relatively unpolluted locations, bacteria or portions of bacteria may represent a major component of the organic aerosols residing in the atmosphere (22,44). Airborne bacteria can have important effects on human health and the productivity of managed and natural ecosystems. For example, bacteria can cause allergic asthma and seasonal allergies, diseases which are increasingly prevalent in developed nations (14, 35), with allergic asthma currently affecting millions of people in the United States (2). Likewise, important plant and livestock pathogens are dispersed through the atmosphere (20, 38) and there is some evidence that bacteria are capable of influencing atmospheric processes by initiating cloud condensation and ice nucleation events, potentially altering precipitation patterns (10, 34). Despite their abundance and likely importance, we have a limited understanding of the quantities and types of bacteria found in the atmosphere (45). With recent advances in high-throughput sequencing, we can now describe the dynamics of airborne bacterial populations (5, 7) and determine likely sources of bacteria in the atmosphere (6), building a more comprehensive understanding of those bacteria found in the atmosphere and the control of their populations.With the present study, we describe bacterial abundances and community composition in the near-surface a...

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Section: Resultssupporting

confidence: 65%

Sources of Bacteria in Outdoor Air across Cities in the Midwestern United States

Bowers

Sullivan

Costello

et al. 2011

Appl Environ Microbiol

247

207

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“…The relative importance of different potential sources of airborne bacteria could be speculated from the general knowledge of the ecology of the dominant bacterial taxa (Franzetti et al, 2011). Rhizobiales and Sphingomonadales were the plant-associated bacteria (Bertolini et al, 2013), especially Sphingomonadales was the common inhabitant of leaves (Murakami et al, 2010). Burkholderiales and Pseudomonadales were the soilinhabiting bacteria (Bertolini et al, 2013).…”

Section: Sources Analysis Based On the Core Orders And Generamentioning

confidence: 99%

Airborne Bacterial Communities of PM2.5 in Beijing-Tianjin-Hebei Megalopolis, China as Revealed by Illumina MiSeq Sequencing: A Case Study

Gao

Pan

2017

Aerosol Air Qual. Res.

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Bacteria are ubiquitous and abundant in the atmosphere and some of them are potential pathogens known to cause diseases or allergies in humans. However, the quantities and compositions of total airborne bacterial community and their relationships with environmental factors remain poorly investigated. Here, a case study of the total airborne bacteria of PM 2.5 collected at six cities in Beijing-Tianjin-Hebei (BTH) megalopolis, China were profiled using quantitative polymerase chain reaction (qPCR) and Illumina MiSeq (PE300) sequencing. qPCR results showed the high abundance of total airborne bacteria of PM 2.5 in BTH, ranging from 4.82 × 10 4 ± 1.58 × 10 3 to 2.64 × 10 5 ± 9.63 × 10 4 cell m -3 air, and averaged 1.19 × 10 5 cell m -3 air. The six PM 2.5 samples were classified into three groups. Proteobacteria, Cyanobacteria, Actinobacteria and Firmicutes were the four dominant phyla of PM 2.5 . 18 common potential pathogens with extremely low percentage (3.61%) were observed, which were dominated by Enterococcus faecium and Escherichia coli. Plants and soil are probably the main sources of bacteria in PM 2.5 , as suggested by the high percentages of Chloroplast, plant-associated bacteria (e.g., Rhizobiales and Sphingomonadales) and soil-inhabiting bacteria (e.g., Burkholderiales and Pseudomonadales). Variation partitioning analysis (VPA) indicated that the atmospheric pollutants explained the most of the variation (31.90%) in community structure of PM 2.5 , followed by meteorological conditions (15.73%) and the chemical compositions of PM 2.5 (11.32%). The case study furthers our understanding of the diversity and composition of airborne bacterial communities of PM 2.5 in BTH, and also identified the main factors shaping the bacterial communities.

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“…Real time PCR has been shown to be a powerful tool for quantification of bacteria in various environments down to the genus level (Khan and Yadav 2004;Skovhus et al 2004;Murakami et al 2010). The SYBR Green real time PCR requires no predetermination of target sequences, and it can be used to detect any double-stranded DNA PCR product (Vitzthum et al 1999;Filion et al 2003).…”

Section: Introductionmentioning

confidence: 99%

“…The SYBR Green real time PCR requires no predetermination of target sequences, and it can be used to detect any double-stranded DNA PCR product (Vitzthum et al 1999;Filion et al 2003). Murakami et al (2010) has successfully applied SYBR Green real time PCR for the quantification of sphingomonads in leaf residues. Denaturing gradient gel electrophoresis (DGGE) has been widely applied to study microbial diversity of soil ecosystems (Li et al 2005;Nakatsu 2007).…”

Section: Introductionmentioning

confidence: 99%

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Abundance and diversity of Sphingomonas in Shenfu petroleum-wastewater irrigation zone, China

Zhou

Zhang

et al. 2011

Environ Sci Pollut Res

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Abundance and Community Structure of Sphingomonads in Leaf Residues and Nearby Bulk Soil

Cited by 11 publications

References 41 publications

Sources of Bacteria in Outdoor Air across Cities in the Midwestern United States

Sources of Bacteria in Outdoor Air across Cities in the Midwestern United States

Airborne Bacterial Communities of PM2.5 in Beijing-Tianjin-Hebei Megalopolis, China as Revealed by Illumina MiSeq Sequencing: A Case Study

Abundance and diversity of Sphingomonas in Shenfu petroleum-wastewater irrigation zone, China

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