Abundance and diversity of Sphingomonas in Shenfu petroleum-wastewater irrigation zone, China

Zhou, Lisha; Li, Hui; Zhang, Ying; Han, Shun; Xu, Hui

doi:10.1007/s11356-011-0552-y

Cited by 16 publications

(13 citation statements)

References 39 publications

(49 reference statements)

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“…A 70-km irrigation channel was constructed in 1960's, and the wastewater mainly comes from an oil refinery. After 50-year exposure to petroleum-containing wastewater, soils in the irrigation zone have been seriously contaminated (Li et al, 2005 ; Zhou et al, 2012 ). Plasmid pSFA231 was isolated from a sediment sample collected from down-stream of the irrigation channel.…”

Section: Methodsmentioning

confidence: 99%

The broad-host-range plasmid pSFA231 isolated from petroleum-contaminated sediment represents a new member of the PromA plasmid family

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Brown

et al. 2015

Front. Microbiol.

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A self-transmissible broad-host-range (BHR) plasmid pSFA231 was isolated from petroleum-contaminated sediment in Shen-fu wastewater irrigation zone, China, using the triparental mating exogenous plasmid capture method. Based on its complete sequence the plasmid has a size of 41.5 kb and codes for 50 putative open reading frames (orfs), 29 of which represent genes involved in replication, partitioning and transfer functions of the plasmid. Phylogenetic analysis grouped pSFA231 into the newly defined PromA plasmid family, which currently includes five members. Further comparative genomic analysis shows that pSFA231 shares the common backbone regions with the other PromA plasmids, i.e., genes involved in replication, maintenance and control, and conjugative transfer. Nevertheless, phylogenetic divergence was found in specific gene products. We propose to divide the PromA group into two subgroups, PromA-α (pMRAD02, pSB102) and PromA-β (pMOL98, pIPO2T, pSFA231, pTer331), based on the splits network analysis of the RepA protein. Interestingly, a cluster of hypothetical orfs located between parA and traA of pSFA231 shows high similarity with the corresponding regions on pMOL98, pIPO2T, and pTer331, suggesting these hypothetical orfs may represent “essential” plasmid backbone genes for the PromA-β subgroup. Alternatively, they may also be accessory genes that were first acquired and then stayed as the plasmid diverged. Our study increases the available collection of complete genome sequences of BHR plasmids, and since pSFA231 is the only characterized PromA plasmid from China, our findings also enhance our understanding of the genetic diversity of this plasmid group in different parts of the world.

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Section: Methodsmentioning

confidence: 99%

The broad-host-range plasmid pSFA231 isolated from petroleum-contaminated sediment represents a new member of the PromA plasmid family

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Brown

et al. 2015

Front. Microbiol.

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“…Water is used in daily activities, which in turn increases the need for efficient wastewater treatment facilities. Due to rapid industrialization, a large quantity of polluted water is generated every year by different industries such as textile (Zheng et al 2012), leather (Natarajan et al 2013), pharmaceutical (Farhadi et al 2012;Boroski et al 2009), heavy metal (Al Aji et al 2012), cadmium removal (Bayar et al 2013;Yilmaz et al 2012), arsenic (Can et al 2012(Can et al , 2014, dairy processing (Idris et al 2012), paint (Singh et al 2015), petroleum (Zhou et al 2012), pulp and paper (Bengtsson et al 2008;Pokhrel and Viraraghavan 2004), distillery (Lucas et al 2009), fertilizer (Srivastava et al 1996), tannery (Houshyar et al 2012), landfill (Wu et al 2011), bakers yeast (Gengec et al 2012), food processing (Pocostales et al 2012), biodiesel (Ngamlerdpokin et al 2011) and olive packaging industries (García-García et al 2011;Kul et al 2015), Pistachio processing industry Fil et al 2014), etc. The pulp and paper industry is a large consumer of fresh water and an important source of wastewater (Ashrafi et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Sono assisted electrocoagulation process for the removal of pollutant from pulp and paper industry effluent

Asaithambi

Aziz

Sajjadi

et al. 2016

Environ Sci Pollut Res

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In the present work, the efficiency of the sonication, electrocoagulation, and sono-electrocoagulation process for removal of pollutants from the industrial effluent of the pulp and paper industry was compared. The experimental results showed that the sono-electrocoagulation process yielded higher pollutant removal percentage compared to the sonication and electrocoagulation process alone. The effect of the operating parameters in the sono-electrocoagulation process such as electrolyte concentration (1-5 g/L), current density (1-5 A/dm), effluent pH (3-11), COD concentration (1500-6000 mg/L), inter-electrode distance (1-3 cm), and electrode combination (Fe and Al) on the color removal, COD removal, and power consumption were studied. The maximum color and COD removal percentages of 100 and 95 %, respectively, were obtained at the current density of 4 A/dm, electrolyte concentration of 4 g/L, effluent pH of 7, COD concentration of 3000 mg/L, electrode combination of Fe/Fe, inter-electrode distance of 1 cm, and reaction time of 4 h, respectively. The color and COD removal percentages were analyzed by using an UV/Vis spectrophotometer and closed reflux method. The results showed that the sono-electrocoagulation process could be used as an efficient and environmental friendly technique for complete pollutant removal.

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“…Previous real-time PCR analysis indicated that all 15 petroleum-contaminated soil samples gave positive signals for Sphingomonas . 11 However, in this study only 12 soils yielded yellow colonies on the selective LB plates with Sm. The number of yellow CFUs ranged from 0.6 × 10 3 to 1.6 × 10 5 CFUs g −1 soil ( Table 1 ).…”

Section: Resultsmentioning

confidence: 51%

“…The physicochemical soil characteristics and the PAH concentrations in the soil were reported in our previous study. 11 Typically, soil samples collected from the up- and mid-stream sites accumulated more petroleum contaminants compared with those from the down-stream sites.…”

Section: Methodsmentioning

confidence: 99%

“…Mineral oil (especially PAHs) was the dominant contaminant in the Shenfu irrigation zone. 10 Our previous study demonstrated that a large percentage of Sphingomonas species was present in the petroleum-contaminated soils of the Shenfu irrigation area using culture-independent methods, 11 indicating a potential link between Sphingomonas and the biodegradation of petroleum compounds in the Shenfu irrigation zone. However, due to the lack of a reliable system for the specific detection and isolation of culturable Sphingomonas , the functional strains in the environmental samples were only found at random.…”

Section: Introductionmentioning

confidence: 99%

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Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities

Zhou

Zhang

et al. 2016

Brazilian Journal of Microbiology

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Members of the Sphingomonas genus are often isolated from petroleum-contaminated soils due to their unique abilities to degrade polycyclic aromatic hydrocarbons (PAHs), which are important for in situ bioremediation. In this study, a combined phenotypic and genotypic approach using streptomycin-containing medium and Sphingomonas-specific PCR was developed to isolate and identify culturable Sphingomonas strains present in petroleum-contaminated soils in the Shenfu wastewater irrigation zone. Of the 15 soil samples examined, 12 soils yielded yellow streptomycin-resistant colonies. The largest number of yellow colony-forming units (CFUs) could reach 105 CFUs g−1 soil. The number of yellow CFUs had a significant positive correlation (p < 0.05) with the ratio of PAHs to total petroleum hydrocarbons (TPH), indicating that Sphingomonas may play a key role in degrading the PAH fraction of the petroleum contaminants at this site. Sixty yellow colonies were selected randomly and analyzed by colony PCR using Sphingomonas-specific primers, out of which 48 isolates had PCR-positive signals. The 48 positive amplicons generated 8 distinct restriction fragment length polymorphism (RFLP) patterns, and 7 out of 8 phylotypes were identified as Sphingomonas by 16S rRNA gene sequencing of the representative strains. Within these 7 Sphingomonas strains, 6 strains were capable of using fluorene as the sole carbon source, while 2 strains were phenanthrene-degrading Sphingomonas. To the best of our knowledge, this is the first report to evaluate the relationship between PAHs contamination levels and culturable Sphingomonas in environmental samples.

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Abundance and diversity of Sphingomonas in Shenfu petroleum-wastewater irrigation zone, China

Cited by 16 publications

References 39 publications

The broad-host-range plasmid pSFA231 isolated from petroleum-contaminated sediment represents a new member of the PromA plasmid family

The broad-host-range plasmid pSFA231 isolated from petroleum-contaminated sediment represents a new member of the PromA plasmid family

Sono assisted electrocoagulation process for the removal of pollutant from pulp and paper industry effluent

Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities

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