Abundance of tigecycline resistance genes and association with antibiotic residues in Chinese livestock farms

Fu, Yulin; Chen, Yiqiang; Liu, Dejun; Yang, Dengfeng; Liu, Zhihai; Wang, Yingyu; Wang, Jiayi; Wang, Xueyang; Xu, Xiaobiao; Li, Xing; He, Jiakang; Jiang, Junyao; Zhai, Weishuai; Huang, Lingli; He, Tao; Xia, Xi; Cai, Chang; Wang, Yang; Jiang, Haiyang

doi:10.1016/j.jhazmat.2020.124921

Cited by 40 publications

(16 citation statements)

References 53 publications

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“…Importantly, we found that the fitness advantage of most evolved strains was generally improved by experimental evolution, accompanied by the increased plasmid stability. Tigecycline is currently only used in human clinical practice, but tet(X4)-positive strains have been widely found in aquaculture environments (Babaei and Haeili, 2021;Fu et al, 2021). In contrast, other tetracyclines such as tetracycline, oxytetracycline, chlortetracycline, and doxycycline are commonly used in veterinary clinics in China (Li et al, 2021a; ), and IncA/Q1 (E) plasmids were competed with a reference strain E. coli TOP10, respectively.…”

Section: Discussionmentioning

confidence: 99%

Large-Scale Analysis of Fitness Cost of tet(X4)-Positive Plasmids in Escherichia coli

Tang

Cai

Jiang

et al. 2022

Front. Cell. Infect. Microbiol.

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Tigecycline is one of important antimicrobial agents for the treatment of infections caused by multidrug-resistant (MDR) Gram-negative bacteria. However, the emergence and prevalence of plasmid-mediated tigecycline resistance gene tet(X4) are threatening human and animal health. Fitness cost elicited by resistance plasmids is a key factor affecting the maintenance and transmission of antibiotic resistance genes (ARGs) in the host. A comparative analysis of the fitness cost of different types of tet(X4)-positive plasmids is helpful to understand and predict the prevalence of dominant plasmids. In this study, we performed a large-scale analysis of fitness cost of tet(X4)-positive plasmids origin from clinical isolates. These plasmids were successfully electroporated into a reference strain Escherichia coli TOP10, and a series of transformants carrying the tet(X) gene were obtained. The effects of tet(X4)-positive plasmids on the growth rate, plasmid stability, relative fitness, biofilm formation, and virulence in a Galleria mellonella model were evaluated. Consequently, we found that these plasmids resulted in varying degrees of fitness cost on TOP10, including delayed bacterial growth and attenuated virulence. Out of these plasmids, tet(X4)-harboring IncFII plasmids showed the lowest fitness cost on the host. Furthermore, by means of experimental evolution in the presence of commonly used drugs in clinic, the fitness cost of tet(X4)-positive plasmids was substantially alleviated, accompanied by increased plasmid stability. Collectively, our data reveal the differential fitness cost caused by different types of tet(X4)-positive plasmids and suggest that the wide use of tetracycline antibiotics may promote the evolution of plasmids.

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Section: Discussionmentioning

confidence: 99%

Large-Scale Analysis of Fitness Cost of tet(X4)-Positive Plasmids in Escherichia coli

Tang

Cai

Jiang

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Tetracycline (TC) is one of the major categories in PhACs. As an ionizable and polar antibiotic, TC plays an important role preventing and treating diseases in livestock farming, although most of it cannot be absorbed by livestock, thus draining into the environment (Fu et al, 2021;Scaria et al, 2021). Our research team previously found that the contents of tetracyclines were significantly higher than that of sulfonamides in Guangdong coastal areas, ranging from 0.26 to 81.54 ng/L (Xu et al, 2019).…”

Section: Introductionmentioning

confidence: 93%

Interactive Effects of Microplastics and Tetracycline on Bioaccumulation and Biochemical Status in Jian Carp (Cyprinus carpio var. Jian)

Wang

Ding³

et al. 2021

Front. Environ. Sci.

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Microplastics (MPs) and tetracycline (TC) are severe emerging pollutants in the aquatic environment. But there is a lack of research to investigate the interactive effects of MPs and TC in vivo. This study used Jian carp (Cyprinus carpio var. Jian) as the model organism to explore the bioaccumulation and biochemical status when exposed to MPs and TC, alone and combined. The accumulation of TC and MPs in intestine, variation of enzyme activities in intestine, and expression of immune-related genes in muscle were evaluated. Our results found the bioaccumulation of MPs was not affected by TC, but the presence of MPs would change the content of TC within 48 h. The superoxide dismutase (SOD) and lactate dehydrogenase (LDH) activity showed that TC-MP combined exposure could reduce oxidative damage to Jian carps compared to MP exposure alone. The integrated biomarker response (IBR) index showed that SOD activity was sensitive to TC-MP exposure. In addition, co-exposure to MPs and TC could alleviate the overexpression of interleukin 1 beta (IL-1β), interleukin 10 (IL-10), transforming growth factor beta (TGF-β), and toll like receptor 2 (TLR-2) induced by TC in muscles. TLR-2 gene has the potential to be the candidate gene reflecting the injury of TC exposure. In conclusion, it is inferred that co-exposure may reduce the toxicity of individual exposure in the living organisms. This study provides essential information for the risk assessment of pollution with MPs and TC, individually and combined, as well as a foundation to investigate the interactive effects of MPs and antibiotics on aquatic ecosystems.

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“…Unfortunately, cocarriage of mobile tet (X) and tmexCD3-toprJ3 has been detected ( Hirabayashi et al, 2021b ). High concentrations of tmexCD1-toprJ1 , and tet (X3/4) was simultaneously detected in fecal samples of food-producing animals ( Fu et al, 2021 ). Clonal dissemination and persistence of both tet (X) and RND efflux pumps gene is ensured since some of the MTR genes [e.g., tet (X3/4/5/6, tmexCD1-toprJ1 and tmexCD3-toprJ3 )] have been detected on chromosomes of bacteria ( Chen et al, 2019a , 2020 ; Li et al, 2020 ; Liu et al, 2020 ; Peng et al, 2020 ; Zhang et al, 2020b ; Wang et al, 2021e ; Zheng et al, 2022 ).…”

Section: Distribution Reservoirs and Traits Of Mobile Tigecycline Res...mentioning

confidence: 99%

“…This would, therefore, prompt the rapid acquisition of resistance genes that possibly often involves the capture of MTR genes (especially by naturally competent organisms scavenging DNA fragments from the environment), by a single event of HGT. In fact, the use of florfenicol and tiamulin in livestock are believed to positively correlate with increasing concentration of mobile tet (X) and HLT efflux pump genes in animal fecal samples ( Fu et al, 2021 ). Nevertheless, some resistance plasmids can capture resistance genes even in the absence of antibiotics selective pressure ( Lopatkin et al, 2016 ).…”

Section: Causes Of Mobile Tigecycline Resistance Developmentmentioning

confidence: 99%

Mobile Tigecycline Resistance: An Emerging Health Catastrophe Requiring Urgent One Health Global Intervention

et al. 2022

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Mobile tigecycline resistance (MTR) threatens the clinical efficacy of the salvage antibiotic, tigecycline (TIG) used in treating deadly infections in humans caused by superbugs (multidrug-, extensively drug-, and pandrug-resistant bacteria), including carbapenem- and colistin-resistant bacteria. Currently, non-mobile tet(X) and mobile plasmid-mediated transmissible tet(X) and resistance-nodulation-division (RND) efflux pump tmexCD-toprJ genes, conferring high-level TIG (HLT) resistance have been detected in humans, animals, and environmental ecosystems. Given the increasing rate of development and spread of plasmid-mediated resistance against the two last-resort antibiotics, colistin (COL) and TIG, there is a need to alert the global community on the emergence and spread of plasmid-mediated HLT resistance and the need for nations, especially developing countries, to increase their antimicrobial stewardship. Justifiably, MTR spread projects One Health ramifications and portends a monumental threat to global public and animal health, which could lead to outrageous health and economic impact due to limited options for therapy. To delve more into this very important subject matter, this current work will discuss why MTR is an emerging health catastrophe requiring urgent One Health global intervention, which has been constructed as follows: (a) antimicrobial activity of TIG; (b) mechanism of TIG resistance; (c) distribution, reservoirs, and traits of MTR gene-harboring isolates; (d) causes of MTR development; (e) possible MTR gene transfer mode and One Health implication; and (f) MTR spread and mitigating strategies.

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Abundance of tigecycline resistance genes and association with antibiotic residues in Chinese livestock farms

Cited by 40 publications

References 53 publications

Large-Scale Analysis of Fitness Cost of tet(X4)-Positive Plasmids in Escherichia coli

Large-Scale Analysis of Fitness Cost of tet(X4)-Positive Plasmids in Escherichia coli

Interactive Effects of Microplastics and Tetracycline on Bioaccumulation and Biochemical Status in Jian Carp (Cyprinus carpio var. Jian)

Mobile Tigecycline Resistance: An Emerging Health Catastrophe Requiring Urgent One Health Global Intervention

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