Abundances of Clinically Relevant Antibiotic Resistance Genes and Bacterial Community Diversity in the Weihe River, China

Wang, Xiaojuan; Gu, Jie; Gao, Hua; Qian, Xiaoqing; Li, Haichao

doi:10.3390/ijerph15040708

Cited by 37 publications

(19 citation statements)

References 60 publications

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“…Meanwhile, Hydrogenophaga might be a potential host for the MRG subtype czcS after both short-term and long-term exposure (Figure 6). This was consistent with results from a previous study reporting that Hydrogenophaga was significantly predominant in Hengshi sediments, with a higher abundance of As, Cu, and Zn resistance genes (Wang X. et al, 2018). Moreover, most of the ARGs and MRGs co-occurred in Acinetobacter and Pseudomonas (Figure 6).…”

Section: Co-occurrence Of Antibiotic Resistance Genes Metal Resistanc...supporting

confidence: 92%

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Shu

et al. 2022

Front. Microbiol.

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Abuse of heavy metals and antibiotics results in the dissemination of metal resistance genes (MRGs) and antibiotic resistance genes (ARGs). Ditch wetlands are important sinks for heavy metals and antibiotics. The relationships between bacterial communities and MRG/ARG dissemination under dual stresses of heavy metals and antibiotics remain unclear. The responses of MRGs and ARGs to the co-selective pressure of cadmium (Cd) and doxycycline (DC) in ditch wetlands were investigated after 7-day and 84-day exposures. In ecological ditches, residual rates of Cd and DC varied from 0.4 to –5.73% and 0 to –0.61%, respectively. The greatest total relative abundance of ARGs was observed in the Cd 5 mg L–1 + DC 50 mg L–1 group. A significant level of DC (50 mg L–1) significantly reduced the total relative abundances of MRGs at a concentration of 5 mg L–1 Cd stress. Redundancy analysis indicated that Cd and DC had strong positive effects on most ARGs and MRGs after a 7-day exposure. Meanwhile, the class 1 integron gene (intI1) exhibited strong positive correlations with most ARGs and cadmium resistance genes (czcA) after an 84-day exposure. Network analysis showed that Acinetobacter and Pseudomonas were the potential dominant host genera for ARGs and MRGs, and tetracycline resistance genes (tetA), czcA, and intI1 shared the same potential host bacteria Trichococcus after an 84-day exposure.

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Section: Co-occurrence Of Antibiotic Resistance Genes Metal Resistanc...supporting

confidence: 92%

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Shu

et al. 2022

Front. Microbiol.

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“…The diversity of MDR isolates was highest at the upstream site and significantly lower at the CSO site during the overflow event (Figure 5). This is consistent with several prior studies that have documented reduced diversity in environments experiencing wastewater pollution [66][67][68]. In our study, nearly all (97%) isolates were Proteobacteria and Bacteroidetes, which are among the phyla most commonly reported in rivers, tap water, and wastewater [69][70][71].…”

Section: Identification and Diversity Of Resistant Bacteriasupporting

confidence: 92%

Diversity of Multidrug-Resistant Bacteria in an Urbanized River: A Case Study of the Potential Risks from Combined Sewage Overflows

Balasa

Levengood

Battistelli

et al. 2021

Water

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Wastewater contamination and urbanization contribute to the spread of antibiotic resistance in aquatic environments. This is a particular concern in areas receiving chronic pollution of untreated waste via combined sewer overflow (CSO) events. The goal of this study was to expand knowledge of CSO impacts, with a specific focus on multidrug resistance. We sampled a CSO-impacted segment of the James River (Virginia, USA) during both clear weather and an active overflow event and compared it to an unimpacted upstream site. Bacteria resistant to ampicillin, streptomycin, and tetracycline were isolated from all samples. Ampicillin resistance was particularly abundant, especially during the CSO event, so these isolates were studied further using disk susceptibility tests to assess multidrug resistance. During a CSO overflow event, 82% of these isolates were resistant to five or more antibiotics, and 44% were resistant to seven or more. The latter statistic contrasts starkly with the upstream reference site, where only 4% of isolates displayed resistance to more than seven antibiotics. DNA sequencing (16S rRNA gene) revealed that ~35% of our isolates were opportunistic pathogens, comprised primarily of the genera Stenotrophomonas, Pseudomonas, and Chryseobacterium. Together, these results demonstrate that CSOs can be a significant source of viable clinically-relevant bacteria to the natural environment and that multidrug resistance is an important understudied component of the environmental spread of antibiotic resistance.

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“…Methods for studying microbial DNA generally use subsamples of each water sample (e.g., from 150-300 ml to 1 L), which are filtered on polycarbonate membrane filters (0.22 μm; X. Wang et al, 2018); in some cases, prefiltration on 30-μm filters is performed to eliminate large particles. The microbial cells are then recovered from the filters (agitation with PBS plus Tween 20 at 28 °C, vortexing, and centrifuging the samples), and the DNA is extracted from the biomass with lysozyme, proteinase K, and phenol:chloroform:isoamylic alcohol (25:24:1).…”

Section: Methods For Studying Ar In River Watermentioning

confidence: 99%

“…A large number of genomic tools are used to determine microbial community composition and the presence of ARGs. For example, culture-independent methods such as PCR, qPCR, high-capacity qPCR arrays, droplet digital PCR (ddPCR), and DNA microarray are frequently used (Larsson et al, 2018;L.-G. Li et al, 2020;X. Wang et al, 2018); and ddPCR and DNA microarray can simultaneously detect a large number of ARGs in a single assay.…”

Section: Methods For Studying Ar In River Watermentioning

confidence: 99%

Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

Grenni

2022

Enviro Toxic and Chemistry

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River ecosystems are very important parts of the water cycle and an excellent habitat, food, and drinking water source for many organisms, including humans. Antibiotics are emerging contaminants which can enter rivers from various sources. Several antibiotics and their related antibiotic resistance genes (ARGs) have been detected in these ecosystems by various research programs and could constitute a substantial problem. The presence of antibiotics and other resistance cofactors can boost the development of ARGs in the chromosomes or mobile genetic elements of natural bacteria in rivers. The ARGs in environmental bacteria can also be transferred to clinically important pathogens. However, antibiotics and their resistance genes are both not currently monitored by national or international authorities responsible for controlling the quality of water bodies. For example, they are not included in the contaminant list in the European Water Framework Directive or in the US list of Water-Quality Benchmarks for Contaminants. Although ARGs are naturally present in the environment, very few studies have focused on non-impacted rivers to assess the background ARG levels in rivers, which could provide some useful indications for future environmental regulation and legislation. The present study reviews the antibiotics and associated ARGs most commonly measured and detected in rivers, including the primary analysis tools used for their assessment. In addition, other factors that could enhance antibiotic resistance, such as the effects of chemical mixtures, the effects of climate change, and the potential effects of the coronavirus disease 2019 pandemic, are discussed.

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Abundances of Clinically Relevant Antibiotic Resistance Genes and Bacterial Community Diversity in the Weihe River, China

Cited by 37 publications

References 60 publications

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Diversity of Multidrug-Resistant Bacteria in an Urbanized River: A Case Study of the Potential Risks from Combined Sewage Overflows

Antimicrobial Resistance in Rivers: A Review of the Genes Detected and New Challenges

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