2005
DOI: 10.1093/pcp/pci006
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Abundant Expression in Vascular Tissue of Plant TAF10, an Orthologous Gene for TATA Box-binding Protein-associated Factor 10, in Flaveria trinervia and Abnormal Morphology of Arabidopsis thaliana Transformants on its Overexpression

Abstract: TAF10 is one of the TATA box-binding protein-associated factors (TAFs), which constitute the TFIID complex. We isolated a plant TAF10 ortholog from a Flaveria trinervia cDNA library, and named it ftTAF10. The ftTAF10 polypeptide contains a histone-fold motif, which is highly conserved among the TAF10s of other organisms. A transiently expressed green fluorescent protein (GFP) fusion protein was translocated into the nuclei of onion epidermal cells, suggesting that the ftTAF10 functions in nuclei. The transcrip… Show more

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Cited by 22 publications
(22 citation statements)
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“…Poly-A1 mRNA was isolated and used to prepare single-strand radiolabelled cDNA as described 22 . Each radiolabelled probe was hybridized to duplicate nylon membranes containing approximately 9,000 phage clones of a F. trinervia leaf cDNA library 23 . Except for the major C 4 marker genes encoding phosphoenolpyruvate carboxylase (PEPC), pyruvate, orthophosphate dikinase (PPDK) and NADP-ME, we isolated 56 clones as novel C 4 -abundant signals, which were classified into 19 genes by determining their cDNA sequences.…”
Section: Methods Summarymentioning
confidence: 99%
“…Poly-A1 mRNA was isolated and used to prepare single-strand radiolabelled cDNA as described 22 . Each radiolabelled probe was hybridized to duplicate nylon membranes containing approximately 9,000 phage clones of a F. trinervia leaf cDNA library 23 . Except for the major C 4 marker genes encoding phosphoenolpyruvate carboxylase (PEPC), pyruvate, orthophosphate dikinase (PPDK) and NADP-ME, we isolated 56 clones as novel C 4 -abundant signals, which were classified into 19 genes by determining their cDNA sequences.…”
Section: Methods Summarymentioning
confidence: 99%
“…For example, only AtTAF6 is expressed at detectable levels in mature pollen and in tricell microgametes (https://www.genevestigator.ethz.ch, Zimmermann et al 2004). In studies where TAF10 from Flaveria trinervia was overexpressed in Arabidopsis, two distinct phenotypes were evident (Furumoto et al 2005). The first was a terminal flower-like phenotype displaying limitation of the indeterminate inflorescence, the presence of chimeric floral organs and dwarf curled leaves.…”
Section: Introductionmentioning
confidence: 99%
“…The TFIID component TAF10 has also been implicated in floral development. Overexpression of both Flaveria trinervia TAF10 or AtTAF10 in Arabidopsis results in floral abnormalities in addition to defects in leaf development (Furumoto et al, 2005;Tamada et al, 2007). Insertional mutants with reduced AtTAF10 levels displayed shorter roots and a high incidence of arrested vegetative meristems (Tamada et al, 2007).…”
Section: Discussionmentioning
confidence: 99%