Accelerated bone formation on photo‐induced hydrophilic titanium implants: an experimental study in the dog mandible

Hirakawa, Yuko; Jimbo, Ryo; Shibata, Yasuaki; Watanabe, Ikuya; Wennerberg, Ann; Saeki, Takashi

doi:10.1111/j.1600-0501.2011.02401.x

Cited by 45 publications

(64 citation statements)

References 30 publications

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“…Moreover, a study using mini-pig model found great bone apposition on hydrophilic surfaces 10 and 15 days post-implant placement (6). This finding corresponds very well with the same parameters assessed in dog mandibles, which showed enhanced bone formation surrounding the hydrophilic surface of implants after 14 days of healing (3).…”

Section: Discussionsupporting

confidence: 79%

“…After a healing period of 28 days, no differences between the groups were observed, and these data are supported by the literature. A previous study (3) found no differences in BA or BIC values between hydrophilic and hydrophobic surfaces after 28 days of implantation. However, Lang at al.…”

Section: Discussionmentioning

confidence: 71%

“…Hydrophilic implant surfaces account for faster wettability, favoring cell-implant contact, the initial phases of wound healing and a cascade of events leading to the osteogenesis (3). At the end, bone apposition increase bone-implant contact (BIC) (4) and improve bonding strength during the early stages of bone repair (5,6).…”

Section: Introductionmentioning

confidence: 99%

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Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

et al. 2017

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The objective of this study was to evaluate the early osseointegration of two different implants surfaces, a sandblasted and acid-etched surface (TN) compared with same geometry and surface roughness modified to be hydrophilic/wettable by conditioning in an isotonic solution of 0.9% sodium-chloride (TA) through histological and histomorphometric analysis after sheep tibia implantation. Forty dental implants, divided in two groups (TN and TA) were placed in the left tibia of twenty healthy, skeletally mature Santa Ines sheep (n=5/experimental period). After 7, 14, 21 and 28 days post-implantation, the samples were removed and the sheep were kept alive. Analysis of resin sections (30 μm) allowed the quantification of bone area (BA) and bone-to-implant contact (BIC). TA group presented nearly 50% increase in BA at 14 days (p<0.001, ANOVA -Tukey's post test) compared with 7 days. The TA presented higher values than the TN for BA and BIC at 14, 21, and 28 days after placement, stabilizing bone healing. TA hydrophilic surface promoted early osseointegration at 14 and 21 days compared to TN, accelerating bone healing period post-implant placement in sheep tibia.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 71%

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Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

et al. 2017

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“…This finding indicated that rutile TiO 2 used in this experiment has stable chemical and physical properties as a biomaterial [18][19][20][21][22][23][24] . One of the reasons for the improved cell proliferation in this study is most likely the difference in surface roughness between the experimental material and control material.…”

Section: Compact Preparationmentioning

confidence: 98%

Proliferation and Alkaline Phosphatase Activity of Osteoblast-like Cells on the Sintered Rutile Titanium Dioxide

Yokoi

Uozumi

Matsuda

et al. 2017

J. Hard Tissue Biology.

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The purpose of this study is creation of biomaterials from titanium dioxide (TiO 2 ). This TiO 2 has known for photocatalysis and osteogenesis. For the purpose of applying this function to orthodontic brackets and coating materials for implant, the relationship between surface of sintered and cell proliferation were examined. In addition, crystal structure and the surface property of sintering TiO 2 were investigated. TiO 2 were sintered at 1300°C for use as samples. We examined surface roughness, x-ray diffraction and scanning electron microscopy to make observations of the surface properties and texture. Moreover, mouse osteoblast-like cell line, MC3T3-E1 was cultured on sintered TiO 2 in order to evaluate the cell proliferation and ALP. For the samples sintered at 1300°C, the crystalline phase of rutile-type TiO 2 was confirmed.5000-fold magnified SEM images of the surface of the unsintered samples, needle-like TiO 2 crystals were pressure welded and showed mutual overlap, with pores occurring among the crystals. Sintering at 1300°C produced numerous small pores. Rutile TiO 2 as a starting material was sintered at 1300°C and subjected to a cell culture experiment in which MC3T3-E1 cells were cultured on the sample, followed by viable cell counting and cell morphology observation on days 7, 14, 21, and 28 of culture. In the test of cell proliferation, sintered at 1300°C samples was found to remarkable cell proliferation even after time had passed. ALP activity of cells on 1300°C TiO 2 sample, the values were 110% and 126% on days 14 and 28 of culture, respectively. These changes were calculated using polystyrene dish as the reference condition. Thus, TiO 2 sintered at 1300°C showed good compatibility and increase in the ALP activity in MC3T3-E1 cells.

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“…All TNTs used in this study were annealed at 450°C for 3 h to crystallize the amorphous TNTs into an anatase structure, and sterilized using ultraviolet irradiation for 2 h. Previous studies have shown that anatase TNTs are beneficial for the nucleation and growth of hydroxyapatite, 27 and when sterilized using ultraviolet irradiation, anatase TNTs can acquire photocatalytic properties accounting for improved cell behavior and early-stage bone formation. 28 In this study, TNTs were loaded with a novel GL13K AMP and a conventional MNA antibiotic. FESEM (Figure 1), FTIR (Figure 3), and LC-MS (Figure 4) analyses confirmed that GL13K and MNA were successfully immobilized onto and into 80 nm TNTs using a simple soaking technique, without any change in physicochemical properties and structure.…”

mentioning

confidence: 99%

Antibacterial activity and cytocompatibility of an implant coating consisting of TiO<sub>2</sub> nanotubes combined with a GL13K antimicrobial peptide

Wang

Chen

et al. 2017

IJN

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Prevention of implant-associated infections at an early stage of surgery is highly desirable for the long-term efficacy of implants in dentistry and orthopedics. Infection prophylaxis using conventional antibiotics is becoming less effective due to the development of bacteria resistant to multiple antibiotics. An ideal strategy to conquer bacterial infections is the local delivery of antibacterial agents. Therefore, antimicrobial peptide (AMP) eluting coatings on implant surfaces is a promising alternative. In this study, the feasibility of utilizing TiO 2 nanotubes (TNTs), processed using anodization, as carriers to deliver a candidate AMP on titanium surfaces for the prevention of implant-associated infections is assessed. The broad-spectrum GL13K (GKIIKLKASLKLL-CONH2) AMP derived from human parotid secretory protein was selected and immobilized to TNTs using a simple soaking technique. Field emission scanning electron microscope, X-ray diffraction, Fourier transform infrared spectroscopy, and liquid chromatography–mass spectrometry analyses confirmed the successful immobilization of GL13K to anatase TNTs. The drug-loaded coatings demonstrated a sustained and slow drug release profile in vitro and eradicated the growth of Fusobacterium nucleatum and Porphyromonas gingivalis within 5 days of culture, as assessed by disk-diffusion assay. The GL13K-immobilized TNT (GL13K-TNT) coating demonstrated greater biocompatibility, compared with a coating produced by incubating TNTs with equimolar concentrations of metronidazole. GL13K-TNTs produced no observable cytotoxicity to preosteoblastic cells (MC3T3-E1). The coating may also have an immune regulatory effect, in support of rapid osseointegration around implants. Therefore, the combination of TNTs and AMP GL13K may achieve simultaneous antimicrobial and osteoconductive activities.

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Accelerated bone formation on photo‐induced hydrophilic titanium implants: an experimental study in the dog mandible

Abstract: The combined applications of plasma fibronectin and PSII to produce hydrophilic titanium surfaces could accelerate early osseointegration.

Cited by 45 publications

References 30 publications

Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

Proliferation and Alkaline Phosphatase Activity of Osteoblast-like Cells on the Sintered Rutile Titanium Dioxide

Antibacterial activity and cytocompatibility of an implant coating consisting of TiO<sub>2</sub> nanotubes combined with a GL13K antimicrobial peptide

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Accelerated bone formation on photo‐induced hydrophilic titanium implants: an experimental study in the dog mandible

Abstract: The combined applications of plasma fibronectin and PSII to produce hydrophilic titanium surfaces could accelerate early osseointegration.

Cited by 45 publications

References 30 publications

Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

Accelerated Healing Period with Hydrophilic Implant Placed in Sheep Tibia

Proliferation and Alkaline Phosphatase Activity of Osteoblast-like Cells on the Sintered Rutile Titanium Dioxide

Antibacterial activity and cytocompatibility of an implant coating consisting of TiO<sub>2</sub>&nbsp;nanotubes combined with a GL13K antimicrobial peptide

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Product

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Antibacterial activity and cytocompatibility of an implant coating consisting of TiO<sub>2</sub> nanotubes combined with a GL13K antimicrobial peptide