Accelerated DNA fragmentation of the denture‐bearing mucosal epithelium in an animal model of diabetes

Maruo, Yukinori; Sugimoto, Tomosada; Oka, Makio; Hara, T.; Sato, Takashi

doi:10.1046/j.1365-2842.2001.00674.x

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…Apoptosis, or programmed cell death with DNA fragmentation, is involved in cellular development and homeostasis. Multiple stimuli influence apoptosis, such as induction of diabetes that affects the regulation of submandibular gland structure which interprets the significant positive immune reaction to caspase-3 in the offspring of diabetic mothers and the diabetic rats themselves in the study of Maruo et al [ 46 ]. Moreover, minimal reaction for α SMA, which is a myoepithelial cell proliferation marker [ 47 ], and PCNA, which denotes nuclear proliferation [ 48 ], in 2w diabetic and 4w diabetic groups demonstrates the degenerative effects of maternal diabetes on the glands of their offspring affecting their structure and function.…”

Section: Discussionmentioning

confidence: 99%

Postnatal changes in the development of rat submandibular glands in offspring of diabetic mothers: Biochemical, histological and ultrastructural study

2018

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Development and maturation of submandibular salivary glands are influenced by intrauterine diabetic environment. Several studies investigated the effects of diabetes on the salivary glands. However, the effects of maternal diabetes on the submandibular glands of the offspring was not properly examined. Therefore, the present study was designed to describe the changes in the development of the submandibular glands of the offspring of diabetic mothers. The submandibular glands of the offspring of Streptozotocin (STZ)-induced diabetic female rats were examined at two and four weeks after birth. Detection of mRNA demonstrated that maternal diabetes affects the level of different indicators. The reduction of expression of epidermal growth factor (EGF); a protein mitogen, cytokeratin 5 (CK5); an epithelial cell progenitor, CK7 and aquaporin 5 (AQP5); differentiation markers and B cell lymphoma 2 (Bcl2); an antiapoptotic marker were found. Increase in Bcl2-associated X protein (Bax); an apoptotic marker was detected. These changes indicate their effects on saliva secretion, glands tumorigenesis, growth of normal oral flora and oral microbes, with decreased protein synthesis and production of xerostomia and dental caries. Loss of normal glandular architecture, significant increase in fibrosis, by the detection of collagen fibers, and stagnation of secretory granules were found with atrophic changes in the acinar cells. Marked defect of polysaccharides in the acinar cells, denoting functional changes, was manifested by significant reduction of the intensity of periodic acid-Schiff (PAS) reaction. The positive immunoreactivity of caspase-3, denoting cellular apoptosis, and minimal reaction of alpha-smooth muscle actin (α SMA) and proliferating cell nuclear antigen (PCNA) were evident in the offspring of diabetic mothers. We conclude that maternal diabetes produces degenerative effects in the structure and function of the submandibular salivary glands of the offspring, reflecting possible influences on their secretory activity affecting oral and digestive health.

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Section: Discussionmentioning

confidence: 99%

Postnatal changes in the development of rat submandibular glands in offspring of diabetic mothers: Biochemical, histological and ultrastructural study

2018

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“…Studies have investigated this interaction by using three models -animals, cell lines that were grown in monolayers, and people. Animal models contribute substantially to the understanding of the biological basis of the development of human oral pathology that follows microorganism infection (46,47). Mouse models have helped to define cellular and molecular targets in the initiation of oral pathology, biochemical pathways involved in promotion of acute and chronic inflammation, and intracellular mechanisms of pathogenesis (48,49).…”

Section: Interaction Between the Host And The Oral Microbial Communitymentioning

confidence: 99%

Interactions between Host and Oral Commensal Microorganisms are Key Events in Health and Disease Status

Rouabhia

2002

Canadian Journal of Infectious Diseases and Medical Microbiolog

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The oral cavity has sometimes been described as a mirror that reflects a person's health. Systemic disease such as diabetes or vitamin deficiency may be seen as alterations in the oral mucosa. A variety of external factors cause changes in the oral mucosa, thus altering mucosal structure and function, and promoting oral pathologies (most frequently bacterial, fungal and viral infections). Little is known, however, about immune surveillance mechanisms that involve the oral mucosa.There is no direct contact between specific immune cells in the basal epithelium and microorganisms in the upper layers of the oral mucosa. The author's hypothesis is that the protective immunity is conveyed through epithelial cells. The present brief review assesses the oral mucosa's role as the main defense in the interactions between the host and the oral microbial community. A unique model was used to investigate these interactions as the cause of oral disease and to develop new treatments that exploit our knowledge of the host-microorganism relationship.

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“…However, the use of immunosuppressed mice reproduce neither the erythematous characteristic of the palate found clinically in DS, nor the systemic condition of immunocompetent denture users. For this reason, some studies have fitted acrylic devices on the rats’ palate to induce DS [ 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. These studies used male rats in contrast to the murine model that employed female mice.…”

Section: Introductionmentioning

confidence: 99%

A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats

Sakima

Vega-Chacón

Cerri

et al. 2022

JoF

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Denture stomatitis (DS) is a common infection in denture wearers, especially women. This study evaluated the induction of DS using acrylic devices attached to the palate of rats combined with inoculation of Candida spp. Immunocompetent male and female rats received a carbohydrate-rich diet. Impressions were taken from the rats’ palate to individually fabricate acrylic devices. Mono- and multispecies biofilms of C. albicans, C. glabrata, and C. tropicalis were grown on the devices, which were then cemented on posterior teeth and kept in the rats’ palate for four weeks. Microbial samples from the palate and the device were quantified. Oral microbiome of rats inoculated with C. albicans was analyzed by 16S rRNA gene sequencing. Log10(CFU/mL) were analyzed by mixed or two-way MANOVA (α = 0.05). Candida spp. and acrylic device did not induce palatal inflammation macroscopically nor microscopically. Although there was an increase (p < 0.001) of the total microbiota and female rats demonstrated higher (p = 0.007) recovery of Candida spp. from the palate, the gender differences were not biologically relevant. The microbiome results indicate an increase in inflammatory microbiota and reduction in health-associated micro-organisms. Although Candida spp. and acrylic device did not induce DS in immunocompetent rats, the shift in microbiota may precede manifestation of inflammation.

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Accelerated DNA fragmentation of the denture‐bearing mucosal epithelium in an animal model of diabetes

Cited by 7 publications

References 17 publications

Postnatal changes in the development of rat submandibular glands in offspring of diabetic mothers: Biochemical, histological and ultrastructural study

Postnatal changes in the development of rat submandibular glands in offspring of diabetic mothers: Biochemical, histological and ultrastructural study

Interactions between Host and Oral Commensal Microorganisms are Key Events in Health and Disease Status

A Denture Use Model Associated with Candida spp. in Immunocompetent Male and Female Rats

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