Accelerated endothelial wound healing on microstructured substrates under flow

Franco, Davide; Milde, Florian; Klingauf, Mirko; Orsenigo, Fabrizio; Dejana, Elisabetta; Poulikakos, Dimos; Cecchini, Marco; Koumoutsakos, Petros; Ferrari, Aldo; Kurtcuoglu, Vartan

doi:10.1016/j.biomaterials.2012.10.007

Cited by 74 publications

(102 citation statements)

References 29 publications

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“…In previous studies, we showed that the interaction of nerve growth factor (NGF)-differentiating PC12 cells with these substrates promotes bipolarity and alignment to the substrate topography by interfering with FA establishment and maturation [29][30][31]. The ability to direct neurite growth depends on both the dimensions of the underlying patterned substrate and, very importantly, on the neuronal cell type [31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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Controlling and improving neuronal cell migration and neurite outgrowth are critical elements of tissue engineering applications and development of artificial neuronal interfaces. To this end, a promising approach exploits nano/microstructured surfaces, which have been demonstrated to be capable of tuning neuronal differentiation, polarity, migration and neurite orientation. Here, we investigate the neurite contact guidance of leech neurons on plastic gratings (GRs; anisotropic topographies composed of alternating lines of grooves and ridges). By high-resolution microscopy, we quantitatively evaluate the changes in tubulin cytoskeleton organization and cell morphology and in the neurite and growth cone development. The topography-reading process of leech neurons on GRs is mediated by filopodia and is more responsive to 4-µm-period GRs than to smaller period GRs. Leech neuron behaviour on GRs is finally compared and validated with several other neuronal cells, from murine differentiated embryonic stem cells and primary hippocampal neurons to differentiated human neuroblastoma cells.

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Section: Introductionmentioning

confidence: 99%

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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“…Understanding and accelerating the mechanisms of wound healing is of fundamental interest for biotechnology and clinical research in repairing invasive medical interventions. Franco et al [99] reported the influence of substrate topography and flow on the efficiency of endothelial regeneration. They found that flow significantly accelerated the healing process of the wound.…”

Section: Wound Healingmentioning

confidence: 98%

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

Zheng

Jiang

2014

Colloids and Surfaces B: Biointerfaces

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“…The temporal variations and absolute magnitude of flow-generated wall shear stress (WSS) and wall deformation (WD) showed a critical impact on all tested ECs activities202122. In particular, the migration and polarization of ECs are directly modulated by the direction and time pattern of flow2324. The stability of substrate adhesions and Vascular Endothelial Cadherin (VEC)-based cell-to-cell junctions is controlled by the absolute WSS value2526.…”

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confidence: 99%

“…The stability of substrate adhesions and Vascular Endothelial Cadherin (VEC)-based cell-to-cell junctions is controlled by the absolute WSS value2526. The EC migration potential upon wound healing is regulated by the flow direction and the resulting WSS values1123. The monolayer response to inflammatory insults similarly depend on flow directionality and WSS.…”

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confidence: 99%

“…Partial access to physiological hemodynamic conditions has been introduced through bioreactors able to produce dynamic patterns of WSS2328, uni- or biaxial stretch (summarized recently in refs 29 and 30) or some combination of these3132. Only few examples reported the concomitant application of flow and uniaxial strain33343536 on ECs but did not explore WSS values higher than 2 Pa.…”

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confidence: 99%

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A Novel Bioreactor System for the Assessment of Endothelialization on Deformable Surfaces

Bachmann

Bernardi

Loosli

et al. 2016

Sci Rep

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The generation of a living protective layer at the luminal surface of cardiovascular devices, composed of an autologous functional endothelium, represents the ideal solution to life-threatening, implant-related complications in cardiovascular patients. The initial evaluation of engineering strategies fostering endothelial cell adhesion and proliferation as well as the long-term tissue homeostasis requires in vitro testing in environmental model systems able to recapitulate the hemodynamic conditions experienced at the blood-to-device interface of implants as well as the substrate deformation. Here, we introduce the design and validation of a novel bioreactor system which enables the long-term conditioning of human endothelial cells interacting with artificial materials under dynamic combinations of flow-generated wall shear stress and wall deformation. The wall shear stress and wall deformation values obtained encompass both the physiological and supraphysiological range. They are determined through separate actuation systems which are controlled based on validated computational models. In addition, we demonstrate the good optical conductivity of the system permitting online monitoring of cell activities through live-cell imaging as well as standard biochemical post-processing. Altogether, the bioreactor system defines an unprecedented testing hub for potential strategies toward the endothelialization or re-endothelialization of target substrates.

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Accelerated endothelial wound healing on microstructured substrates under flow

Cited by 74 publications

References 29 publications

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Precise manipulation of cell behaviors on surfaces for construction of tissue/organs

A Novel Bioreactor System for the Assessment of Endothelialization on Deformable Surfaces

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