Accelerated skin wound healing by soy protein isolate–modified hydroxypropyl chitosan composite films

Zhao, Yanan; Wang, Zijian; Zhang, Qiang; Chen, Feixiang; Yue, Zhi-Yi; Zhang, Tiantian; Deng, Hongbing; Huselstein, Céline; Anderson, Debbie P.; Chang, Peter R.; Li, Yinping; Chen, Yun

doi:10.1016/j.ijbiomac.2018.06.195

Cited by 71 publications

(33 citation statements)

References 51 publications

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“…Evaluation of cytotoxicity is an important cell viability method to validate the potential use of the chitosan-sodium carbonate complex in medical bioengineering. As the L929 fibroblast cell line is commonly used to determine the cell proliferation rate, the effect of the chitosan-sodium carbonate complex was assessed in this cell line by using the MTT assay [ 28 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

A Novel Complex of Chitosan–Sodium Carbonate and Its Properties

Qian

Shu

et al. 2018

Marine Drugs

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Chitosan has excellent properties, as it is nontoxic, mucoadhesive, biocompatible, and biodegradable. However, the poor water solubility of chitosan is a major disadvantage. Here, a novel chitosan-sodium carbonate complex was formed by adding a large amount of sodium carbonate to a chitosan/acetic acid solution, which is water-soluble. Fourier transform infrared spectroscopy, energy dispersive spectrometry, scanning electron microscopy, and solid-state nuclear magnetic resonance techniques were used to detect and characterize the aforementioned complex, which appeared to be a neat flake crystal. Solid-state nuclear magnetic resonance (SSNMR) was used to verify the connections between carbonate, sodium ions, and the protonated amino group in chitosan on the basis of 13C signals at the chemical shift of 167.745 ppm and 164.743 ppm. Further confirmation was provided by the strong cross-polarization signals identified by the SSNMR 2D 13C–1H frequency-switched Lee–Goldberg heteronuclear correlation spectrum. The cytotoxicity of a film prepared using this complex was tested using rat fibroblasts. The results show that the film promoted cell proliferation, which provides evidence to support its nontoxicity. The ease of film-forming and the results of cytocompatibility testing suggest that the chitosan-sodium carbonate complex has the potential for use in tissue engineering.

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Section: Resultsmentioning

confidence: 99%

A Novel Complex of Chitosan–Sodium Carbonate and Its Properties

Qian

Shu

et al. 2018

Marine Drugs

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“…The good ability of SM1127 EPS to promote skin wound healing may be attributed to its properties. SM1127 EPS has an excellent moisture-retention ability, which is better than that of the functional biopolymers generally used in wound dressings, such as HA, chitosan, and sodium alginate [24], and thus can provide cells a moist environment to favor cell migration and wound healing [33]. In addition, infection and inflammation of wounds usually cause the generation of an excess of reactive oxygen species (ROS), which destroy DNA structure, oxidize proteins and lipids, and hinder wound healing [34].…”

Section: Discussionmentioning

confidence: 99%

Promotion of Wound Healing and Prevention of Frostbite Injury in Rat Skin by Exopolysaccharide from the Arctic Marine Bacterium Polaribacter sp. SM1127

et al. 2020

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Many marine microorganisms synthesize exopolysaccharides (EPSs), and some of these EPSs have been reported to have potential in different fields. However, the pharmaceutical potentials of marine EPSs are rarely reported. The EPS secreted by the Artic marine bacterium Polaribacter sp. SM1127 has good antioxidant activity, outstanding moisture-retention ability, and considerable protective property on human dermal fibroblasts (HDFs) at low temperature. Here, the effects of SM1127 EPS on skin wound healing and frostbite injury prevention were studied. Scratch wound assay showed that SM1127 EPS could stimulate the migration of HDFs. In the full-thickness cutaneous wound experiment of Sprague–Dawley (SD) rats, SM1127 EPS increased the wound healing rate and stimulated tissue repair detected by macroscopic observation and histologic examination, showing the ability of SM1127 EPS to promote skin wound healing. In the skin frostbite experiment of SD rats, pretreatment of rat skin with SM1127 EPS increased the rate of frostbite wound healing and promoted the repair of the injured skin significantly, indicating the good effect of SM1127 EPS on frostbite injury prevention. These results suggest the promising potential of SM1127 EPS in the pharmaceutical area to promote skin wound healing and prevent frostbite injury.

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“…Such findings are particularly interesting as optical, mechanical, adhesive, electrical and various other physicochemical properties of films can be further altered with the changes in the surface morphology [52]. In fact, a rough surface may have benefits for skin regeneration, as it might increase the film's adhesion capacity and thus promote a faster wound healing process [53,54]. Furthermore, the atomic force microscopy technique was used to further investigate the surface microstructure of the films.…”

Section: Sem and Afmmentioning

confidence: 99%

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

et al. 2020

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Chitosan films entrapped with the Mansoa hirsuta fraction (CMHF) was developed as a new dressing for wound care. The chromatographic profile of the M. hirsuta fraction (MHF) was evaluated by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, and the results showed that MHF is rich in acid triterpenes. Physicochemical characterization of the films prepared using the solvent casting method was performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetry (TGA), differential scanning calorimetry (DCS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and mechanical properties. CMHF exhibited characteristic bands of both chitosan and MHF, revealing a physical mixture of both. CMHF presented an amorphous nature, thermostability, and dispersion of MHF in the chitosan matrix, resulting in a rough structure. Incorporation of M. hirsuta fraction into chitosan matrix favorably enhanced the mechanical performance and films thickness. The in vivo wound treatment with CMHF for seven days showed a characteristic area of advanced healing, re-epithelization, cell proliferation, and collagen formation. Furthermore, wound closure reached 100% contraction after 10 days of treatment with modulation of interleukins. The incorporation of M. hirsuta fraction into chitosan films was advantageous and showed great potential for stimulating wound repair and regeneration.

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Accelerated skin wound healing by soy protein isolate–modified hydroxypropyl chitosan composite films

Cited by 71 publications

References 51 publications

A Novel Complex of Chitosan–Sodium Carbonate and Its Properties

A Novel Complex of Chitosan–Sodium Carbonate and Its Properties

Promotion of Wound Healing and Prevention of Frostbite Injury in Rat Skin by Exopolysaccharide from the Arctic Marine Bacterium Polaribacter sp. SM1127

Chitosan Film Containing Mansoa hirsuta Fraction for Wound Healing

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