Accelerated Stability Test of Snakehead Fish Extract (Channa striata) and Kelulut Honey (heterotrigona itama) Ointment Combination with Tween 80 and Span 80 as an Emulsifying Agent

Abstract

The combination of Snakehead Fish and Kelulut Honey has activity in accelerating the wound healing process. The ointment with active ingredients of Snakehead Fish extract and Kelulut Honey showed separation from the active ingredient and its base. The addition of tween 80 and span 80 is expected to increase the stability of the ointment preparation by keeping the water-phase droplets distributed uniformly on the ointment base. This study aimed to determine the effect of adding Tween 80 and Span 80 on the stability of the ointment preparation. The ointment was made by varying Tween 80 and span 80 by 2,5%: 5%: 7,5% and control (active ingredients without the addition of tween 80 and Span 80) as a comparison. The preparation was subjected to an accelerated stability test using a temperature of 40 ° ± 2 ° C / Relative humidity 75% ± 5% for 28 days by observing the organoleptic test, adhesion, spreadability, homogeneity test, pH test, and protection power. The data obtained were analyzed statistically using One Way Anova. The analysis showed that the addition of tween 80 and span 80 with a total concentration of 5% and 7,5% had a significant effect in increasing the spreadability, decreasing adhesion, and reducing the pH of the preparation. Formulas that meet the requirements in this study are F2 and F3, which have an average spreadability of 5,11 cm and 5,25 cm, adhesion of 96 seconds and 51 seconds, and pH of 6,02 and 5,02.

The Response Surface Methodology for Assessment of HLB Values of Mixtures of Non-Ionic Surfactants Using Parameters from Their π-A Isotherms

“…The use of Span 85 is suggested in drug forms containing betamethasone [16] and curcumin [17]. Span 80 mixed with Tween 80 was used in preparations containing fish oil extracts and honey [18]. In the case of mixtures of surfactants, the HLB value may be influenced to varying degrees by both the ELM experimental parameter and the SM experimental parameter.…”

Uji Stabilitas Sifat Fisik Salep Kombinasi Ekstrak Ikan Gabus, Teripang Emas Menggunakan HPMC

“…Stabilitas sangat krusial untuk mengklaim kualitas produk, keamanan, dan kemanjuran. Penelitian sebelumnya menerangkan bahwa formulasi salep ekstrak ikan gabus (Channa striata) dan madu kelulut (Heterotrigona itama) memperoleh sediaan dengan kondisi stabilitas, sifat fisik, dan konsistensi memenuhi persyaratan (Taurina et al, 2021. Hasil orientasi yang telah dilakukan dalam formula sebelumnya menerangkan penurunan konsistensi sediaan yang merupakan dampak dari penambahan ekstrak teripang emas.…”