Accelerating the Peroxidase-Like Activity of Gold Nanoclusters at Neutral pH for Colorimetric Detection of Heparin and Heparinase Activity

Hu, Lianzhe; Liao, Hong; Feng, Lingyan; Wang, Min; Fu, Wensheng

doi:10.1021/acs.analchem.8b00885

Cited by 205 publications

(80 citation statements)

References 44 publications

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“…For example, TMB oxidation implemented by different AuNPs shows an optimum activity at pH values between 3.5 and 4, whereas its optimum temperature is commonly set between 40 and 50 °C ( Fig. 4a) [103][104][105][106][107]. These observations, again, cannot be extrapolated to different peroxidase substrates, i.e., 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) oxidation takes place optimally at lower pH values, while 4-aminoantipyrine (AAP) oxidation is improved at basic pH values [103].…”

Section: Ph and Temperature Dependencementioning

confidence: 99%

Gold Nanozymes: From Concept to Biomedical Applications

et al. 2020

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In recent years, gold nanoparticles have demonstrated excellent enzyme-mimicking activities which resemble those of peroxidase, oxidase, catalase, superoxide dismutase or reductase. This, merged with their ease of synthesis, tunability, biocompatibility and low cost, makes them excellent candidates when compared with biological enzymes for applications in biomedicine or biochemical analyses. Herein, over 200 research papers have been systematically reviewed to present the recent progress on the fundamentals of gold nanozymes and their potential applications. The review reveals that the morphology and surface chemistry of the nanoparticles play an important role in their catalytic properties, as well as external parameters such as pH or temperature. Yet, real applications often require specific biorecognition elements to be immobilized onto the nanozymes, leading to unexpected positive or negative effects on their activity. Thus, rational design of efficient nanozymes remains a challenge of paramount importance. Different implementation paths have already been explored, including the application of peroxidase-like nanozymes for the development of clinical diagnostics or the regulation of oxidative stress within cells via their catalase and superoxide dismutase activities. The review also indicates that it is essential to understand how external parameters may boost or inhibit each of these activities, as more than one of them could coexist. Likewise, further toxicity studies are required to ensure the applicability of gold nanozymes in vivo. Current challenges and future prospects of gold nanozymes are discussed in this review, whose significance can be anticipated in a diverse range of fields beyond biomedicine, such as food safety, environmental analyses or the chemical industry.

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Section: Ph and Temperature Dependencementioning

confidence: 99%

Gold Nanozymes: From Concept to Biomedical Applications

et al. 2020

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“…From the few decades, gold nanoparticles (AuNPs) with intrinsic enzyme‐mimetic activity hold great promise from catalysis to nanomedicine. [ 20–22 ] The previous study has shown that the catalytic properties of AuNPs correspond to the correlation: the smaller the diameter, the more active sites are exposed. [ 22 ] Thus, it is highly expected that ultrasmall AuNPs (UsAuNPs) may possess high enzyme‐mimetic activity.…”

Section: Introductionmentioning

confidence: 99%

In Situ Fabrication of Ultrasmall Gold Nanoparticles/2D MOFs Hybrid as Nanozyme for Antibacterial Therapy

Younis

Zhou

et al. 2020

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As one of the common reactive oxygen species, H2O2 has been widely used for combating pathogenic bacterial infections. However, the high dosage of H2O2 can induce undesired damages to normal tissues and delay wound healing. In this regard, peroxidase‐like nanomaterials serve as promising nanozymes, thanks to their positive promotion toward the antibacterial performance of H2O2, while avoiding the toxicity caused by the high concentrations of H2O2. In this work, ultrasmall Au nanoparticles (UsAuNPs) are grown on ultrathin 2D metal–organic frameworks (MOFs) via in situ reduction. The formed UsAuNPs/MOFs hybrid features both the advantages of UsAuNPs and ultrathin 2D MOFs, displaying a remarkable peroxidase‐like activity toward H2O2 decomposition into toxic hydroxyl radicals (·OH). Results show that the as‐prepared UsAuNPs/MOFs nanozyme exhibits excellent antibacterial properties against both Gram‐negative (Escherichia coli) and Gram‐positive (Staphylococcus aureus) bacteria with the assistance of a low dosage of H2O2. Animal experiments indicate that this hybrid material can effectively facilitate wound healing with good biocompatibility. This study reveals the promising potential of a hybrid nanozyme for antibacterial therapy and holds great promise for future clinical applications.

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“…For instance, the highest eCL factor values were obtained for 2.5-fold dilution presenting data of 7.6 ± 0.2, 14.4 ± 0.1, 22.2 ± 0.5, 29.8 ± 0.1, and 18.1 ± 0.7 for 2.6, 8, 13, 25, and 38 nm of cross-linked GNPs, respectively. The mechanism behind luminol reaction catalytic activity is attributed to the metal nanoparticles’ surface charge properties [32]. Conventional citrate-reduced GNPs possess a negative charge that electrostatically repulses both anionic luminol and hydroperoxide ions, thus significantly reducing the overall CL signal [16].…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

Nirala

Shtenberg

2019

Biomolecules

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Bovine mastitis (BM) is a frequent disease in the dairy industry that causes staggering economical losses due to decreased milk production and increased health care costs. Traditionally, BM detection depends on the efficacy and reliability of analytical techniques that measure somatic cell counts (SCC), detect pathogens, and reveal inflammatory status. Herein, we demonstrate the detection of bovine haptoglobin, a well-documented acute phase protein for evaluating BM clinical status, by utilizing hemoglobin-binding capacity within luminol chemiluminescence (CL) system. The resulting haptoglobin–hemoglobin complex reduces the CL signal proportionally to inherent haptoglobin concentrations. Different sizes of cross-linked gold nanoparticles (GNPs) were examined for enhanced CL (eCL) signal amplification, presenting over 30-fold emitted radiation enhancement for optimized size within real milk samples with respect to nanoparticle-free assay. The eCL values were proportionally related to nanoparticle size and content, influenced by SCC and pathogen type (e.g., Escherichia coli and coagulase-negative staphylococci). The optimized bioassay showed a broad linear response (1 pg mL−1–10 µg mL−1) and minute detection limit of 0.19 pg mL−1, while presenting quantitative performance in agreement with commercial ELISA kit. Finally, the resulting optimized eCL concept offers an efficient label-free detection of haptoglobin biomarker, offering means to diagnose the severity of the associated diseases.

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Accelerating the Peroxidase-Like Activity of Gold Nanoclusters at Neutral pH for Colorimetric Detection of Heparin and Heparinase Activity

Cited by 205 publications

References 44 publications

Gold Nanozymes: From Concept to Biomedical Applications

Gold Nanozymes: From Concept to Biomedical Applications

In Situ Fabrication of Ultrasmall Gold Nanoparticles/2D MOFs Hybrid as Nanozyme for Antibacterial Therapy

Gold Nanoparticle Size-Dependent Enhanced Chemiluminescence for Ultra-Sensitive Haptoglobin Biomarker Detection

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