Acceleration of bone regeneration in bioactive glass/gelatin composite scaffolds seeded with bone marrow-derived mesenchymal stem cells over-expressing bone morphogenetic protein-7

Kargozar, Saeid; Hashemian, Seyed Jafar; Soleimani, Mansooreh; Milan, Peiman Brouki; Khalaj, Vahid; Samadikuchaksaraie, Ali; Hamzehlou, Sepideh; Katebi, Amir Reza; Latifi, Noorahmad; Mozafari, Masoud; Baino, Francesco

doi:10.1016/j.msec.2017.02.097

Cited by 83 publications

(39 citation statements)

References 51 publications

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“…The results of histology and immunohistochemistry indicate that the pure Gel/BaG nanocomposite scaffolds have good biocompatibility and osteoconductivity. These findings are consistent with Kargozar et al report that Gel/BaG nanocomposite scaffolds are well biocompatible and enhance new bone formation especially in the presence of MSCs. Periosteal osteoprogenitor cells and blood vessels grow into the defect site and new bone tissue regenerate and gradually infiltrate into the implant along with the resorption of the scaffold material.…”

Section: Discussionsupporting

confidence: 93%

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Kazemi

Azami

Johari

et al. 2018

Int J Applied Ceramic Tech

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In our previous study, a three‐dimensional gelatin/bioactive glass nanocomposite scaffold with a total porosity of about 85% and pore sizes ranging from 200 to 500 μm was prepared through layer solvent casting combined with lamination technique. The aim of this study was to evaluate in vitro biocompatibility and in vivo bone regeneration potential of these scaffolds with and without endothelial cells when implanted into a critical‐sized rat calvarial defect. MTT assay, SEM observation, and DAPI staining were used to evaluate cell viability and adhesion in macroporous scaffolds and results demonstrated that the scaffolds were biocompatible enough to support cell attachment and proliferation. To investigate the in vivo osteogenesis of the scaffold, blank scaffolds and endothelial/scaffold constructs were implanted in critical‐sized defects, whereas in control group defects were left untreated. Bone regeneration and vascularization were evaluated at 1, 4, and 12 weeks postsurgery by histological, immunohistochemical, and histomorphometric analysis. It was shown that both groups facilitated bone growth into the defect area but improved bone regeneration was seen with the incorporation of endothelial cells. The data showed that the porous Gel/BaG nanocomposite scaffolds could well support new bone formation, indicating that the proposed strategy is a promising alternative for tissue‐engineered bone defects.

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Section: Discussionsupporting

confidence: 93%

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Kazemi

Azami

Johari

et al. 2018

Int J Applied Ceramic Tech

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“…This suggests the important role of ionic release on cellular behavior. 7 In addition, based on previous studies, it is believed that matrix mineralization is associated with the release of BG ionic products which are known to stimulate osteogenic differentiation hDPSC 7,8,12,26 ; however, this requires further evaluation to confirm this finding.…”

Section: Discussionmentioning

confidence: 99%

Creation of a unique architectural structure of bioactive glass sub‐micron particles incorporated in a polycaprolactone/gelatin fibrous mat; characterization, bioactivity, and cellular evaluations

Fanaee

Labbaf

Houreh

et al. 2019

J Biomedical Materials Res

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In this study, submicron, monodispersed, spherical bioactive glass (BG) particles with a mean diameter of 720 ± 80 nm were produced through sol–gel process. The prepared BG particles were successfully incorporated into 70/30 (W/W) ratio of polycaprolactone/gelatin (PCL/GT) nanofibrous mats (250 ± 60 nm) through electrospinning to obtain a unique architectural structure for the first time. To enhance biodegradation and stability of the scaffolds, crosslinking using gluteraldehyde was applied. The structure, wettability, hydroxyapatite (HA) formation, cell adhesion, cell viability and osteogenic potential of the fibrous mats were evaluated. A continuous, uniform and hydrophilic structure of PCL/GT/BG was obtained. The fibers were found to be bioactive as they formed HA on their surface after immersion in simulated body fluid. The unique structure significantly reduced HA formation time to 5 days. For in vitro investigations, human dental pulp stem cells (hDPSCs) were cultured on PCL/GT and PCL/GT/BG fibrous mats. Results demonstrated good cell attachment after 4 and 24 h with no significant levels of cytotoxicity during 10 days of culture. Alizarin red staining was applied to quantitatively analyze the potential of PCL/GT/BG in osteogenic differentiation of hDPSCs. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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“…Osteogenic cell sheets incorporating gelatin had upregulated expression of osteogenic markers such as BMP-7, TGF-β, AP, osteocalcin, and osteopontin in osteogenically differentiated Ad-MSCs (Kim et al, 2017). Similarly, virally transfected BMP-7-overexpressing MSCs had rapid osteogenic differentiation (Xue et al, 2015), which induced bone regeneration in critical-size bone defects in rats (Kargozar et al, 2017). In the present study, we found that the addition of 0.5% gelatin strongly upregulated the expression of BMP-7, up to 5-to 8-fold higher than that of the MSCs without gelatin, as well as activating the wnt/β-catenin pathway.…”

Section: Discussionmentioning

confidence: 99%

Gelatin positively regulates the immunosuppressive capabilitiesof adipose-derived mesenchymal stem cells

et al. 2017

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IntroductionAdipose-derived mesenchymal stem cells (Ad-MSCs) are highly multipotent cells with a high potential for differentiation into multiple cell types (Vieira et al., 2010;Park et al., 2012;Mellor et al., 2015). Ad-MSCs produce various growth factors that contribute to injury healing and tissue regeneration (Salgado et al., 2010). The antiinflammatory, immunomodulatory, and antioxidant properties of mesenchymal stem cells (MSCs) make them desirable candidates for cell-based therapies (Soleymaninejadian et al., 2012).The usefulness of MSCs has been selectively enhanced by either manipulating the culture media composition (Langenbach and Handschel, 2013) or by gene transduction (Liu et al., 2015). Pretreatment with proinflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), and interleukin-6 (IL-6), enhances the immunosuppressive capacity of MSCs by upregulating the expression of indoleamine-2,3-dioxygenase (IDO), which inhibits the proliferation of lymphocytes (Hoogduijn et al., 2010). Gelatin sponges incorporating high amounts of β-tricalcium phosphate (β TCP) have been used to stimulate rat bone marrow MSC proliferation and osteogenic differentiation by stimulating higher alkaline phosphatase (AP) activity and osteocalcin expression (Takahashi et al., 2005). Similarly, a scaffold prepared by mixing chitosan with gelatin, β TCP, and hydroxyapatite (HA) has also been used to induce MSC adhesion, differentiation, and proliferation (Zhao et al., 2006).MSCs cultured in a hyperoxic environment have enhanced viability and paracrine therapeutic capabilities (Song et al., 2010), as well as increased expression of the naturally occurring antioxidant stanniocalcin-1 (SC-1), which prevents oxygen-induced bronchopulmonary dysplasia in rats (Waszak et al., 2012). HO-1, a potent antioxidant enzyme, can be induced in undifferentiated MSCs treated with hemin, which renders the MSCs more resistant to oxidative injury (Barbagallo et al., 2008). Osteogenic growth peptide (OGP) induces HO-1 expression in human bone marrow MSCs, which induces osteogenic differentiation as evidenced by elevated levels of bone morphogenic protein-2 (BMP-2) and osteonectin (Vanella et al., 2010(Vanella et al., , 2013. We have previously reported that gelatin-induced osteogenic cell sheets had active cell proliferation with abundant extracellular matrix and upregulation of osteogenic markers (Kim et al., 2016). In the present study, we evaluated the effects of gelatin on

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Acceleration of bone regeneration in bioactive glass/gelatin composite scaffolds seeded with bone marrow-derived mesenchymal stem cells over-expressing bone morphogenetic protein-7

Cited by 83 publications

References 51 publications

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Bone Regeneration in rat using a gelatin/bioactive glass nanocomposite scaffold along with endothelial cells (HUVECs)

Creation of a unique architectural structure of bioactive glass sub‐micron particles incorporated in a polycaprolactone/gelatin fibrous mat; characterization, bioactivity, and cellular evaluations

Gelatin positively regulates the immunosuppressive capabilitiesof adipose-derived mesenchymal stem cells

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