Acceptability of pig xenografts by patients with type 1 diabetes and the general population.

Section: Discussionmentioning

confidence: 97%

Attitudinal study of organ xenotransplantation in patients on the kidney and liver transplant waiting list in a country with a high rate of deceased donation

Martı́nez-Alarcón

Rı́os

Pons

et al. 2011

“…Many studies have reported the potential of porcine islet xenotransplantation in the treatment of diabetes. 1,2 However, the xenotransplantation of porcine islets also has many hurdles, such as immune rejection, the failure of the initial transplantation, and the early loss of transplanted islet cells. [3][4][5] Recently, one of the significant problems in islet xenotransplantation is islet death, including β-cell loss after transplantation.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have reported the potential of porcine islet xenotransplantation in the treatment of diabetes . However, the xenotransplantation of porcine islets also has many hurdles, such as immune rejection, the failure of the initial transplantation, and the early loss of transplanted islet cells .…”

Section: Introductionmentioning

confidence: 99%

Enhanced effect of human mesenchymal stem cells expressing human TNF‐αR‐Fc and HO‐1 gene on porcine islet xenotransplantation in humanized mice

Lee

Song

Shin

et al. 2017

Background: Porcine islet xenotransplantation is considered an attractive alternative treatment for type 1 diabetes mellitus. However, it is largely limited because of initial rejection due to Instant Blood-Mediated Inflammatory Reaction (IBMIR), oxidative stress, and inflammatory responses. Recently, soluble tumor necrosis factor-ɑ receptor type I (sTNF-αR) and heme oxygenase (HO)-1 genes (HO-1/sTNF-αR) have been shown to improve the viability and functionality of porcine islets after transplantation. Methods:In this study, genetically modified mesenchymal stem cells (MSCs) expressing the HO-1/sTNF-αR genes (HO-1/sTNF-αR-MSC) were developed using an adenoviral system, and porcine islet viability and function were confirmed by in vitro tests such as GSIS, AO/PI, and the ADP/ATP ratio after coculturing with HO-1/sTNF-αR-MSCs. Subsequently, isolated porcine islets were transplanted underneath the kidney capsule of diabetic humanized mice without MSCs, with MSCs or with HO-1/sTNF-αR-MSCs.Results: According to the results, the HO-1/sTNF-αR-MSC-treated group exhibited improved survival of porcine islets and could reverse hyperglycemia more than porcine islets not treated with MSCs or islets cotransplanted with MSCs. Moreover, the HO-1/sTNF-αR-MSC group maintained its morphological characteristics and the insulin secretion pattern of transplanted porcine islets similar to endogenous islets in immunocompetent humanized mice. Conclusions:Our results suggest that HO-1/sTNF-αR-MSCs are efficient tools for porcine islet xenotransplantation, and this study may provide basic information for pre-clinical animal models and future clinical trials of porcine islet xenotransplantation. K E Y W O R D Sheme oxygenase-1, islet, mesenchymal stromal cell, TNF-αR-Fc, xeno-transplantation

“…;(6) If the pig cells do not rule out the possibility of transmission of diseases by viruses, would you accept the implant of pig cells? ;(7) Do you think that living with pig cells in your body will cause you to have psychological problems? ; (8) Do you think that with scientific progress the procedure of pig cell implantation could cure the disease?…”

mentioning

confidence: 99%

Short Communication: Pig islet xenotransplantation acceptance in a Latin-American diabetic population

Abalovich

Wechsler

Lara

et al. 2010

Progress in porcine islet xenotransplantation has been accompanied by studies on acceptance of this new procedure by patients, health professionals or the general public. Such studies have not been done in the Latin-American population. We conducted a questionnaire in 108 diabetes patients (insulin-dependent, n = 53; insulin-independent, n = 55) in a public hospital in Argentina. The questions addressed the general perception of the xenotransplant procedure and specific items related to the outcome (achieving insulin independence, improvement in metabolic control, delay in emergence of diabetic complications, need for repeat procedures, potential of transfer of infectious viruses, association with psychological problems, and anticipated success in relation to achieving a cure). Eighty-six (79%) of the patients accepted islet xenotransplantation; this incidence was not different for insulin-dependent or insulin-independent patients, patients with or without complications, or patients with good or poor metabolic control. Also, over 75% of patients accepted the procedure if this is only associated with a reduction in insulin requirement, if the procedure just delays but not prevents the onset of complications, or if the procedure needs to be performed every 6 months. Fifty-seven percent of patients indicated acceptance even if the potential transmission of a virus infection cannot be completely ruled out: this outcome was not affected by the outbreak of the H1N1 flu epidemic during the conduct of this study. Forty percent of patients indicated that living with porcine cells in their body could give psychological problems. We conclude that this population of Latin-American diabetic patients shows a high acceptance rate of a porcine islet xenotransplantation product.