Accessible Telemedicine Diagnostics with ELISA in a 3D Printed Pipette Tip

Sharafeldin, Mohamed; Kadimisetty, Karteek; Bhalerao, Ketki R; Bist, Itti; Jones, Abby; Chen, Tianqi; Lee, Norman H.; Rusling, James F.

doi:10.1021/acs.analchem.9b01284

Cited by 30 publications

(27 citation statements)

References 62 publications

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“…Generally, this strategy exploits microchannels to facilitate the manipulation of small amount of fluids for ELISA. For example, fluoropolymer microcapillary film, disposal sensor array, − and 3D-printed pipette tip systems all use parallel microchannel arrays to perform multiplexed testing. Lab-on-a-disc, , “mChip,” , SlipChip, , and thermoelastic microvalve platforms detect different analytes in discrete parallel chambers connected by microchannels.…”

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confidence: 99%

Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA)

Huffman

Curtin

et al. 2020

Anal. Chem.

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Enzyme-linked immunosorbent assay (ELISA) is the gold standard method for protein biomarkers. However, scaling up ELISA for multiplexed biomarker analysis is not a trivial task due to the lengthy procedures for fluid manipulation and high reagent/sample consumption. Herein, we present a highly scalable multiplexed ELISA that achieves a similar level of performance to commercial single-target ELISA kits as well as shorter assay time, less consumption, and simpler procedures. This ELISA is enabled by a novel microscale fluid manipulation method, composable microfluidic plates (cPlate), which are comprised of miniaturized 96-well plates and their corresponding channel plates. By assembling and disassembling the plates, all of the fluid manipulations for 96 independent ELISA reactions can be achieved simultaneously without any external fluid manipulation equipment. Simultaneous quantification of four protein biomarkers in serum samples is demonstrated with the cPlate system, achieving high sensitivity and specificity (∼ pg/mL), short assay time (∼1 h), low consumption (∼5 μL/well), high scalability, and ease of use. This platform is further applied to probe the levels of three protein biomarkers related to vascular dysfunction under pulmonary nanoparticle exposure in rat’s plasma. Because of the low cost, portability, and instrument-free nature of the cPlate system, it will have great potential for multiplexed point-of-care testing in resource-limited regions.

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confidence: 99%

Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA)

Huffman

Curtin

et al. 2020

Anal. Chem.

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“…Inset: (c) Fully transparent 3D printed pipette tips filled with different color food dyes attached to a multi tip pipette; (d) steps involved in the pre coating showing the immobilization of capture antibodies on the inner walls of the tips coated with chitosan followed by the sandwich immunoassay and the generation of the CL signal and colorimetry; (e) Signal capture and processing flow for both colorimetry and CL using a smartphone and a microplate reader. Reproduced with permission from [29]. Copyright (2019) American Chemical Society.…”

Section: D Printed Ecl/cl Sensorsmentioning

confidence: 99%

“…Proteins PF-4 and PSA were studied in this example, giving LODs of 0.5 pg/mL for both along with broad dynamic ranges [ 109 ]. We also developed an assay using CL for multiplexed ELISA in 3D printed pipette tips ( Figure 8 B) [ 29 ]. Both colorimetric and chemiluminescence detection methods were considered in the novel TIP ELISA approach.…”

Section: 3d Printed Ecl/cl Sensorsmentioning

confidence: 99%

“…Additive manufacturing, also known as 3D printing, is making rapid inroads in manufacturing, and advanced fabrications that are quickly moving into production [23]. 3D printing has been utilized in development and fabrication of sensors for detection of glucose [24], drugs [25], trace elements [26], neurotransmitters [27], nucleic acids [28], and proteins [29]. The vast scope and innovative nature of 3D printing in development of biosensors is backed by the versatility of options provided by the immense progress in the design and production of desktop 3D printers.…”

Section: Introductionmentioning

confidence: 99%

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3D-Printed Immunosensor Arrays for Cancer Diagnostics

Sharafeldin

Kadimisetty

Bhalerao

et al. 2020

Sensors

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Detecting cancer at an early stage of disease progression promises better treatment outcomes and longer lifespans for cancer survivors. Research has been directed towards the development of accessible and highly sensitive cancer diagnostic tools, many of which rely on protein biomarkers and biomarker panels which are overexpressed in body fluids and associated with different types of cancer. Protein biomarker detection for point-of-care (POC) use requires the development of sensitive, noninvasive liquid biopsy cancer diagnostics that overcome the limitations and low sensitivities associated with current dependence upon imaging and invasive biopsies. Among many endeavors to produce user-friendly, semi-automated, and sensitive protein biomarker sensors, 3D printing is rapidly becoming an important contemporary tool for achieving these goals. Supported by the widely available selection of affordable desktop 3D printers and diverse printing options, 3D printing is becoming a standard tool for developing low-cost immunosensors that can also be used to make final commercial products. In the last few years, 3D printing platforms have been used to produce complex sensor devices with high resolution, tailored towards researchers’ and clinicians’ needs and limited only by their imagination. Unlike traditional subtractive manufacturing, 3D printing, also known as additive manufacturing, has drastically reduced the time of sensor and sensor array development while offering excellent sensitivity at a fraction of the cost of conventional technologies such as photolithography. In this review, we offer a comprehensive description of 3D printing techniques commonly used to develop immunosensors, arrays, and microfluidic arrays. In addition, recent applications utilizing 3D printing in immunosensors integrated with different signal transduction strategies are described. These applications include electrochemical, chemiluminescent (CL), and electrochemiluminescent (ECL) 3D-printed immunosensors. Finally, we discuss current challenges and limitations associated with available 3D printing technology and future directions of this field.

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“…Whereas many approaches are based on various synthesis steps, the automatic combination of many complex materials can easily be realized by e.g., additive manufacturing or threedimensional (3D) printing [1]. A large variety of 3D printed sensors have been developed and manufactured recently in the field of biotechnology e.g., for detection of glucose [2], selected medicament [3], trace elements [4], neurotransmitters [5], nucleic acids [6] and/or proteins [7].…”

Section: Introductionmentioning

confidence: 99%

Additive Manufacturing as a Means of Gas Sensor Development for Battery Health Monitoring

et al. 2021

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Lithium-ion batteries (LIBs) still need continuous safety monitoring based on their intrinsic properties, as well as due to the increase in their sizes and device requirements. The main causes of fires and explosions in LIBs are heat leakage and the presence of highly inflammable components. Therefore, it is necessary to improve the safety of the batteries by preventing the generation of these gases and/or their early detection with sensors. The improvement of such safety sensors requires new approaches in their manufacturing. There is a growing role for research of nanostructured sensor’s durability in the field of ionizing radiation that also can induce structural changes in the LIB’s component materials, thus contributing to the elucidation of fundamental physicochemical processes; catalytic reactions or inhibitions of the chemical reactions on which the work of the sensors is based. A current method widely used in various fields, Direct Ink Writing (DIW), has been used to manufacture heterostructures of Al2O3/CuO and CuO:Fe2O3, followed by an additional ALD and thermal annealing step. The detection properties of these 3D-DIW printed heterostructures showed responses to 1,3-dioxolan (DOL), 1,2-dimethoxyethane (DME) vapors, as well as to typically used LIB electrolytes containing LiTFSI and LiNO3 salts in a mixture of DOL:DME, as well also to LiPF6 salts in a mixture of ethylene carbonate (EC) and dimethyl carbonate (DMC) at operating temperatures of 200 °C–350 °C with relatively high responses. The combination of the possibility to detect electrolyte vapors used in LIBs and size control by the 3D-DIW printing method makes these heterostructures extremely attractive in controlling the safety of batteries.

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Accessible Telemedicine Diagnostics with ELISA in a 3D Printed Pipette Tip

Cited by 30 publications

References 62 publications

Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA)

Composable Microfluidic Plates (cPlate): A Simple and Scalable Fluid Manipulation System for Multiplexed Enzyme-Linked Immunosorbent Assay (ELISA)

3D-Printed Immunosensor Arrays for Cancer Diagnostics

Additive Manufacturing as a Means of Gas Sensor Development for Battery Health Monitoring

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