Accessions of Solanum tuberosum ssp. andigena show differences in photosynthetic recovery after drought stress as reflected in gene expression profiles

Watkinson, Jonathan I.; Hendricks, Lori; Sioson, Allan A.; Vásquez-Robinet, Cecilia; Stromberg, Verlyn K.; Heath, Lenwood S.; Schuler, Mary A.; Bohnert, Hans J.; Bonierbale, Merideth; Grene, Ruth

doi:10.1016/j.plantsci.2006.07.010

Cited by 63 publications

(43 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In contrast, the more widely investigated antioxidant genes, such as the APXs and SODs (Lin et al 2007;Watkinson et al 2006), did not respond to flood stress in this study. This was probably due to the limited number of clones that were sequenced, differences in the timing and method of selection, and/or differences between the "S" and "M" libraries used for screening.…”

Section: Discussioncontrasting

confidence: 82%

Identification of Flooding-Response Genes in Eggplant Roots by Suppression Subtractive Hybridization

Lin

Chan

et al. 2009

Plant Mol Biol Rep

View full text Add to dashboard Cite

Roots of eggplant (Solanum melongena L.) cv. Pingtong Long Eggplant (EG117) were subjected to ten flooding treatments. Messenger RNAs from plants subjected to flooding periods for 0.25 to 1 and 3 to 12 h as well as unflooded plants (control) were used as the tester and driver samples, respectively. Following suppression subtractive hybridization, polymerase chain reaction (PCR) products were ligated into a cloned vector for Escherichia coli-competent cell transformation, followed by nested PCR screening of positive cDNA inserts. PCR products of these unique clones were digested with Sau3AI to identify any differentially displayed banding patterns. Recombinant cDNA clones were then picked for plasmid extraction and then sequenced. Using BLAST and Gene Ontology database searches, 25 of these unigenes were found to be involved in metabolism, regulation, stress, and development categories, whereas nine novel genes with unknown functions that may have potential roles in flooding stress in eggplant were also identified. Five differentially expressed gene transcripts with known functions were randomly selected for real-time reverse-transcription quantitative PCR to investigate plants subjected to flooding stress from 0 to 72 h. All genes were upregulated in flood treatments (15 min to 72 h) when compared to the control. These findings indicated that flood-induced genes were closely related to various metabolic pathways and involved in genetic regulation of flood stress response.

show abstract

Section: Discussioncontrasting

confidence: 82%

Identification of Flooding-Response Genes in Eggplant Roots by Suppression Subtractive Hybridization

Lin

Chan

et al. 2009

Plant Mol Biol Rep

View full text Add to dashboard Cite

show abstract

“…Several authors have reported that limited soil water availability may affect the potato plant at all developmental stages, resulting in earlier crop maturity and decrease of plant growth, tuber yield, number of tubers per plant, tuber size and tuber quality (MacKerron and Jefferies 1988;Tourneux et al 2003). Recent studies have mainly focused on identifying drought tolerant varieties (varieties with limited decline in tuber yields upon drought) as well as unraveling drought tolerant traits either by physiological (Tourneux et al 2003) or molecular approaches (Watkinson et al 2006, Schafleitner et al 2007). Many researchers identified Quantitative Trait Loci (QTLs) for different traits like tuber dormancy (van den Berg et al 1996), tuber yield and starch content (Schäfer-Pregl et al 1998), tuber shape (van Eck et al 1994), tuber flesh color (Bonierbale et al 1988), tuber skin color (Gebhardt et al 1989(Gebhardt et al , 1991, yield, agronomic and quality traits (Bradshaw et al 2008) in potato under normal conditions.…”

Section: Introductionmentioning

confidence: 99%

In vitro screening and QTL analysis for drought tolerance in diploid potato

et al. 2011

View full text Add to dashboard Cite

Drought stress is a major abiotic constraint limiting crop production worldwide. Screening for drought tolerance and the traits that enhance drought tolerance is not straightforward in large mapping populations. In this study, we investigated the possibility of screening a mapping population in vitro for PEG-induced water deficit stress and recovery potential. We have measured several shoot and root growth parameters or traits in the C 9 E diploid potato mapping population. Significant variation was observed for genotype-specific responses to water deficit and recovery potential. Genetic variation and heritability estimates were high to very high for the measured traits depending on growth conditions. In order to identify potato QTLs for drought tolerance and recovery potential an SNP marker-rich integrated linkage map was used. A total of 23 QTLs were detected under control, stress and recovery treatments explaining 10.3-22.4% of the variance for each phenotypic trait. Among these, 10 QTLs were located on chromosome 2. Three QTLs involved in the important trait root to shoot ratio were identified on linkage groups 2, 3 and 8. These loci explained together 41.1% of the variance for this trait, and may be breeding targets for stress tolerance and yield in the field as well. The SNP markers derived from EST sequences underlying these QTLs led to the identification of putative candidate genes for further study in potato. This study constitutes the first knowledge of in vitro screening of a mapping population for drought tolerance in potato.

show abstract

“…Results of gene expression profiling experiments with microarrays pointed toward detoxification of reactive oxygen species as one of the key traits endorsing survival and tuber production under water stress. Genes encoding enzymes such as superoxide dismutase, ascorbate peroxidase, catalase, glutathione peroxidase, and peroxiredoxin were strongly induced under drought in tolerant accessions (Watkinson et al 2006;Schafleitner et al 2007b;Mane et al 2008;Vasquez-Robinet et al 2008). Additionally, compounds with active oxygen scavenger activity, such as sugar alcohols, amino acids, and an array of secondary metabolites, accumulated under water stress in drought tolerant potato and presumably mitigate the deleterious effects of active oxygen species in the cells .…”

Section: Potato Responses To Climate Change Effects-heat Drought Anmentioning

confidence: 99%

Adaptation of the Potato Crop to Changing Climates

Schafleitner

Ramírez

Jarvis

et al. 2011

Crop Adaptation to Climate Change

View full text Add to dashboard Cite

Accessions of Solanum tuberosum ssp. andigena show differences in photosynthetic recovery after drought stress as reflected in gene expression profiles

Cited by 63 publications

References 50 publications

Identification of Flooding-Response Genes in Eggplant Roots by Suppression Subtractive Hybridization

Identification of Flooding-Response Genes in Eggplant Roots by Suppression Subtractive Hybridization

In vitro screening and QTL analysis for drought tolerance in diploid potato

Adaptation of the Potato Crop to Changing Climates

Contact Info

Product

Resources

About