Acclimatization of In Vitro Banana Seedlings Using Root-Applied Bio-Nanofertilizer of Copper and Selenium

Abstract

The production of in vitro banana transplants has become an important practice in the global banana production. Proper and enough nutrients are needed for banana production particularly during the acclimatization period. To avoid the environmental problem resulting from the chemical fertilizers, nanofertilizers of Se and Cu were separately applied during the acclimatization of banana. The biological form of nano-Cu (50 and 100 mg L−1) and nano-Se (25, 50, 75, and 100 mg L−1) were studied on acclimatized banana transplants under greenhouse conditions. Both applied nanofertilizers enhanced the growth of transplant by 10.9 and 12.6% for dry weight after nano-Se and nano-Cu application up to 100 mg L−1, respectively. The survival rate was also increased by increasing applied doses of both nanofertilizers up to 100 mg L−1, whereas the highest survival rate (95.3%) was recorded for nano-Cu. All studied photosynthetic pigments and its fluorescence were improved by applying nanofertilizers. Studied antioxidant enzymatic activities (CAT, PPO, and POX) were also increased. A pH decrease in the growing medium was noticed after applying nano-Cu, which may explain the high bioavailability of studied nutrients (N, P, K, Cu, Fe, Se, and Zn) by banana transplants.

Biological Nanofertilizers to Enhance Growth Potential of Strawberry Seedlings by Boosting Photosynthetic Pigments, Plant Enzymatic Antioxidants, and Nutritional Status

“…Foliar or soil application of bio-nanofertilizers can support crop production under abiotic and/or biotic stress by improving the nutritional status and antioxidant system in treated plants. These benefits have been documented with selenium (Se) [ 6 , 7 , 8 , 9 , 10 ], copper (Cu) [ 11 ], and zinc (Zn) [ 12 , 13 , 14 , 15 ]. Under natural conditions and human activities, the distribution of NPs in different agroecosystems is considered an important global issue and still needs more extensive investigations in the hydrosphere, including in groundwater, lakes, rivers, and pore water [ 16 ], the atmosphere [ 17 ], the lithosphere, including mainly soils and sediments [ 18 ], and in the biosphere, including mainly plants, micro-organisms, and even humans [ 19 , 20 ].…”

“…There are studies on bio-nano-copper as a guard against plant pathogens such as on absinthium ( Artemisia absinthium L.) [ 23 ], ginger ( Zingiber officinale Roscoe) [ 24 ], and guar ( Cyamopsis tetragonoloba L.) [ 25 ]. However, there are very few studies on the effects of Se and CuO bio-nanofertilizers on other crops such as banana [ 11 ] and tomato [ 26 ]. The uptake of nanofertilizers through soil or foliar application mainly depends on the characterization of the soil, plant species, and the kind of nanofertilizer [ 27 ].…”

Biological Nanofertilizer for Horticultural Crops: A Diagrammatic Mini-Review

“…Depending on the kind of nanofertilizers and the applied dose, impacts may either inhibit or activate enzymes, which control the uptake of water by seedlings or seeds, level of gibberellin, and production of abscisic acid (Feregrino-Perez et al 2018). Other physiological, biochemical and antioxidant properties of horticultural crops have been enhanced after application of nanofertilizers (mainly biological ones) such as bionano-Se and -Cu on banana (Shalaby et al 2022a) and tomato (Saffan et al 2022) and bionano-Se on radish (Huang et al 2023). Therefore, the applications of nanotechnology in agriculture, and particularly horticulture, may provide sustainable solutions for many problems facing this sector (Bala et al 2022;Nongbet et al 2022).…”

“…Recent research has included applying biological nanofertilizers to enhance the growth of many crops under different stress conditions, such as heavy metals (Ahmadi -Nouraldinvand et al 2022;Zhu et al 2022), salinity (El-Badri et al 2022;Ghazi et al 2022), biotic stress (Abou-Salem et al 2022), as well as under nonstressful conditions (Yadav et al 2022;Huang et al 2023). Several crops have been investigated such as tobacco (Zsiros et al 2019), Chrysanthemum morifolium (Seliem et al 2020), lettuce (Kohatsu et al 2021), cucumber (Shalaby et al 2021), Brassica chinensis (Zhu et al 2022), rapeseed (El-Badri et al 2022, wheat (Sheoran et al 2021;Ghazi et al 2022), sugar beet (Abou-Salem et al 2022, guar seedling (Ahmadi-Nouraldinvand et al 2022), banana seedlings (Shalaby et al 2022a), and radish (Huang et al 2023). This mini-review focuses on biological nanofertilizers and their impact on horticultural crops production.…”

Preparation of Seaweed Nanopowder Particles Using Planetary Ball Milling and Their Effects on Some Secondary Metabolites in Date Palm (Phoenix dactylifera L.) Seedlings

“…Accordingly, direct exposure of wheat plants to nano-chitosan NPK fertilizer induced an increased yield production [ 11 ]. Moreover, the advantages of nanofertilizers in terms of fruit quality, productivity, shelf life and lowering nutrient loss in soil after the period of crop harvest have been affirmed [ 12 , 13 ].…”