Acclimatization of Micropropagated Plants to Ex Vitro Conditions

Pospóšilová, J.; Tichá, Ingrid; Kadleček, Petr; Haisel, D.; Plzáková, Š.

doi:10.1023/a:1002688208758

Cited by 356 publications

(153 citation statements)

References 87 publications

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“…Однако культивирование растений in vitro способствует появлению ряда морфоло-гических, анатомических и физиологических аномалий у микропобегов, затрудняющих пере-вод регенерантов в условия ex vitro (Pospisilova et al, 1999;Hazarika, 2006). Следствием этого являются низкая частота ризогенеза in vitro и высокая гибель регенерантов при адаптации к нестерильным условиям теплиц или открытого грунта, ограничивающие использование метода клонального микроразмножения для массово-го воспроизводства ценных генотипов (Chandra et al, 2010).…”

Section: Introductionunclassified

Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

2018

turczaninowia

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Ключевые слова: адаптация ex vitro, гидропонная установка, индолил-3-масляная кислота, укоренение ex vitro, Rhododedron.Аннотация. Укоренение и адаптация являются лимитирующими стадиями для клонального микроразмно-жения растений, особенно у древесных. Представленное исследование направлено на изучение влияния раз-личных обработок индолил-3-маслянной кислотой (ИМК) на укоренение и адаптацию микропобегов шести морозостойких генотипов рододендронов в условиях in vitro и ex vitro. Испытывали два метода применения ИМК: непосредственное культивирование на среде Андерсона (АМ), дополненной 25,0 мкМ ИМК и 4-часо-вую импульсную обработку раствором 148,0 мкМ ИМК. После импульсной обработки ИМК микропобеги переносили либо в условия in vitro на безгормональную АМ, либо в условия ex vitro, используя гидропонику или смесь торфа и песка (1:1). Максимальный процент укоренения in vitro после импульсной обработки полу-чен у вечнозеленых сортов 'Helsinki University' (75 %) и 'Haaga' (67 %), в то время как процент укоренения дальневосточных видов R. sichotense, R. mucronulatum и R. schlippenbachii составил всего 30-40 %. Укоре-нение ex vitro в гидропонике оказалось эффективным для вечнозеленых сортов; микропобеги R. sichotense, напротив, укоренялись слабо. Наилучший результат для всех исследуемых генотипов получен под действием импульсной обработки с последующим укоренением ex vitro в смеси торфа и песка ('Helsinki University' -100 %, R. mucronulatum -89 %, R. sichotense -84 %, и R. schlippenbachii -60 %). В результате исследования вы-явлены генотипические различия, проявляющиеся в частоте и интенсивности ризогенеза при всех вариантах укоренения. Разработанный протокол показал преимущества укоренения ex vitro: высокое качество корневой системы и надземной части, а также сокращение периода адаптации. Таким образом, предложенный метод может использоваться как для селекции и сохранения генетических ресурсов рододендронов, так и для ком-мерческого размножения. Keywords: ex vitro acclimatization, ex vitro rooting, hydroponic system, indolyl-3-butyric acid, Rhododendron. Rooting and acclimatization toSummary. Rooting and acclimatization are limiting stages in clonal micropropagation, especially of woody plant species. This study aimed to evaluate effect of different treatments with indole-3-butyric acid (IBA) on in vitro and ex vitro rooting and acclimatization of microshoots of six winter-hardy rhododendron genotypes. Two methods of IBA applications: direct cultivation on Anderson's medium (AM), supplemented with 25.0 μМ IBA and 4-h liquid-pulse treatment with solution of 148.0 μM IBA were tested. After IBA pulse treatment microshoots were transferred either

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Section: Introductionunclassified

Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

2018

turczaninowia

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“…Micropropagation has been widely used for the rapid multiplication of many plant species, but their more widespread use is limited by a high percentage of plants lost or damaged when transferred to greenhouse or field conditions [1].…”

Section: Introductionmentioning

confidence: 99%

“…The process of acclimatization of plants grown in vitro proves to be one of the most important phases in a biofactory, when the sugarcane plantlet is extracted from the laboratory to the greenhouses for the acclimatization, the change to different environmental conditions has the consequence that these are very susceptible to different stress, because they have not developed or have not adapted their organs to the new conditions, so they need to respond with new anatomical, morphological and physiological characteristics [1] [2] [3] [4].…”

Section: Introductionmentioning

confidence: 99%

Dose of Paclobutrazol in the Growth of Sugarcane Seedlings in Vitro in the Acclimatization Stage

Valdés¹,

Ruvalcaba²,

Ayala-Tafoya³

et al. 2017

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“…Nevertheless, ultimate success of in vitro produced plantlets depends upon the successful transfer and establishment of plants in ex vitro conditions. High loss or damage of in vitro raised plants can occurred when transferred to ex vitro conditions because of the transfer shock [6]. This is due to the exposure of plants to many new ex vitro situations such as low humidity, high level of irradiation, water deficit because of the poor hydraulic conductivity of the roots and low root-stem connection [7].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, plants under in vitro condition need to be acclimatized by different options. The threats for survival in ex vitro can be overcome by acclimatizing the plants with gradual lowering air humidity temperature, air flow and irradiation level [6].…”

Section: Introductionmentioning

confidence: 99%

Acclimatization of in Vitro Propagated Pineapple (Ananas comosuss (L.), var. Smooth cayenne) Plantlets to ex Vitro Condition in Ethiopia

Mengesha¹,

Ayenew²,

Tadesse³

2013

AJPS

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Pineapple (Ananas comosuss, var. Smooth cayenne), which is a popular tropical fruit, is propagated vegetatively. Conventional propagation alone does not provide clean and adequate planting material demanded in Ethiopia. Recently, in vitro multiplication has become a promising technique for large-scale production. However, the acclimatization to the external environment procedure impedes the efficiency, which needs carefully optimized acclimatization techniques. We report optimized acclimatization procedures following first-and second-stage hardening methods for in vitro pineapple plantlets. Primarily, Jiffy-7 peat pellet allowed growing plants vigorously and provided above 8% survival rate over soil mix. Nevertheless, in Ethiopia, soil mix is cheaper and locally accessible. The primarily acclimatized plantlets are needed to be hardened further for better establishment and survival in the field. Black polybag and polysleeve pots filled with soil mix were evaluated in the greenhouse. A significant difference was obtained between pots for number of roots and substrate weight. Polybags had higher root number than polysleeves and saved about 27% of substrates per plant, which is a reduction of 25% of total transportation cost. Hence, the soil mix and polybags were found to be preferable over substrates and pots, for subsequent in vitro pineapple acclimatization.

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Acclimatization of Micropropagated Plants to Ex Vitro Conditions

Cited by 356 publications

References 87 publications

Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

Dose of Paclobutrazol in the Growth of Sugarcane Seedlings <i>in Vitro</i> in the Acclimatization Stage

Acclimatization of <i>in Vitro</i> Propagated Pineapple (<i>Ananas comosuss</i> (L.), var. Smooth cayenne) Plantlets to <i>ex Vitro</i> Condition in Ethiopia

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Acclimatization of Micropropagated Plants to Ex Vitro Conditions

Cited by 356 publications

References 87 publications

Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

Rooting and acclimatization to ex vitro conditions of regenerants of frost-resistant members of Rhododendron

Dose of Paclobutrazol in the Growth of Sugarcane Seedlings &lt;i&gt;in Vitro&lt;/i&gt; in the Acclimatization Stage

Acclimatization of &lt;i&gt;in Vitro&lt;/i&gt; Propagated Pineapple (&lt;i&gt;Ananas comosuss&lt;/i&gt; (L.), var. Smooth cayenne) Plantlets to &lt;i&gt;ex Vitro&lt;/i&gt; Condition in Ethiopia

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Dose of Paclobutrazol in the Growth of Sugarcane Seedlings <i>in Vitro</i> in the Acclimatization Stage

Acclimatization of <i>in Vitro</i> Propagated Pineapple (<i>Ananas comosuss</i> (L.), var. Smooth cayenne) Plantlets to <i>ex Vitro</i> Condition in Ethiopia