Acclimatization of the endangered Mexican epiphytic orchid, Laelia speciosa (H.B.K.) Schltr

Ortega-Loeza, Martha Mireya; Salgado‐Garciglia, Rafael; Gómez‐Alonso, Carlos; Ávila-Díaz, Irene

doi:10.14712/23361964.2015.46

Cited by 7 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to sudden change of environment during growth period the ex-vitro transfer of plants cause damage, so for that hardening period is must which can be given under in vitro conditions or when transferred to polyhouse [10]. At this phase, in vitro raised plantlets are quite susceptible to losses [11]. There is the modification of various features of physiology and morphology during hardening process in plantlets viz., the number of stomata get reduced, the increase in the thickness of leaves, increase in number of chloroplasts and the enhanced vigour of roots [12].…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Bio-hardening of in vitro raised plants of Geneva (G.) series clonal rootstocks of apple (Malus X domestica Borkh.)

Manzoor

Sultan

Kumar

et al. 2023

Preprint

View full text Add to dashboard Cite

Background:The success of tissue culture-raised plantlets lies in their transfer from in vitro to in vivo phase as the step of hardening helps to improve the adaptive behavior of in vitro grown plantlets to the natural environment. But the overall success rates are quite low. ResultsThe present research has been done through bio-inoculation of two apple clonal rootstocks viz., G.41 and G.214 during hardening with Phosphate Solubilizing bacteria(B1) containing Azotobacter chroococcum and Bacillus polymyxawith four concentrations prepared by dissolving 1.0 ml,2.0ml,3.0ml and 4.0ml liquid formulation of Phosphate Solubilizing bacteria solution separately in 10.0 ml distilled water and adding that mixture to ellepot medium with cocopeat moss and Vascular Arbuscular Mycorrhiza (B2) containing mycorrhizal propagules @ 60 spores/g with four concentrations prepared by dissolving 1.0 mg,2.0mg,3.0mg and 4.0mg powdered formulation of Vascular Arbuscular Mycorrhiza separately in 10.0 ml distilled water and adding that mixture to ellepot medium with cocopeat moss. Conclusion: After comparing two different bio-inoculants on two different rootstocks, it was found that out of the two rootstocks,G.41 showed maximum hardening with 4.0 ml of Phosphate Solubilizing bacteria than G.214.Meanwhile, G.214 depicted maximum hardening rates with 2.0 mg of Vascular Arbuscular Mycorrhiza than G.214.Thus, a protocol has been standardized for improving the hardening success under in vivo conditions for commercial purposes in both rootstocks.

show abstract

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: Bio-hardening of in vitro raised plants of Geneva (G.) series clonal rootstocks of apple (Malus X domestica Borkh.)

Manzoor

Sultan

Kumar

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Stomata, as regulators of gaseous and water exchange, have developed a specific structure and unique physiology, both functionally coordinated to result in movement of guard cells. When stomatal aperture open, it allows CO2 to enter the leaf and results in water loss through transpiration (Ortega-Loeza et al 2011;Beck 2012). Predovich (2012) compares stomata density of the epiphytic Pleurothallis aristata and Maxillaria sp.…”

Section: Resultsmentioning

confidence: 99%

Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition

Garvita

Wawangningrum

2020

Biodiversitas

View full text Add to dashboard Cite

Abstract. Garvita RV, Wawangningrum H. 2020. Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition. Biodiversitas 21: 1116-1121. In vitro micropropagation is an efficient biotechnological strategy for conservation and commercial plantlet production. The ultimate success of in vitro microporapagation depends on the ability to transfer plants out of culture, known as acclimatization stage. The morphology, anatomy and physiology of plantlets in in vitro culture conditions is different from the plants in environment condition (greenhouse), therefore they are unable to compete with soil microbes and to cope with the environmental conditions. The aim of this research is to determine stomata morphology and distribution of Paraphalaenopsis spp (Orchidaceae) from in vitro growth and greenhouse conditions. The leaf stomata cells of Paraphalaenopsis spp. (Paraphalaenopsis serpentilingua, Paraphalaenopsis labukensis and Paraphalaenopsis laycockii) was undertaken by observing the stomata preparation of the upper leaf and lower leaf by using microscope fitted with optic visual seven. The descriptive method was used to describe the stomata morphology by observing the epidermis of longitudinal section and stomata length of upper and lower leaf by using a microscope. The results showed that these plants had anomocytic type of stomata cells. The distributions of stomata are in the upper and lower epidermis leaf in all Paraphalaenopsis spp from in vitro growth and greenhouse.

show abstract

“…e conservation of endangered orchid species threatened with extinction in the wild can be aided by in vitro propagation, a procedure that enables massive production of plants for ex situ conservation, reintroduction or commercial purposes, thus reducing the pressure on natural populations (Ávila-Díaz et al, 2009;Lando et al, 2016;Lesar et al, 2012;Ortega-Loeza et al, 2011).…”

Section: Micromorphological Leaf Changes and Antioxidant Defense Syst...mentioning

confidence: 99%

Structural Adaptation and Antioxidant Response of Guarianthe bowringiana (O’Brien) Dressler & W. E. Higgins (Orchidaceae Juss.) Seedlings During Ex Vitro Acclimatization

et al. 2021

View full text Add to dashboard Cite

Guarianthe bowringiana is one of the oldest samples cultivated at NBG’s orchid unit glasshouses since 1970s. An efficient protocol for asymbiotic in vitro seed germination of G. bowringiana has previously been established. Given that acclimatization is a crucial step in micropropagation, this study assesses the structural adaptation and antioxidant response of G. bowringiana seedlings during ex vitro acclimatization to ex vitro conditions. The leaf surface micromorphology of the G. bowringiana juvenile plants propagated in vitro from seeds as well as the leaves of adult plants cultivated in glasshouse were analyzed using scanning electron microscopy. The levels of lipid peroxidation (TBARS level), superoxide dismutase (SOD) activity, and the photosynthetic activity were monitored for seven days from the transfer of seedlings from the in vitro cultivation vessels as they are markers indicating the response of the leaves of in vitro propagated G. bowringiana plants to oxidative stress during the early stages of acclimatization to ex vitro conditions. During the initial 2 days of the monitored acclimatization period (0–7 days), the level of photosynthetic pigments (chlorophyll a , b , and carotenoid content) increased, followed by an insignificant increase during the successive period (by the seventh day) of acclimatization. At the same time, the level of the tested antioxidant enzyme (SOD) exhibited an increasing trend throughout the acclimatization period. The SOD activities in the leaves of G. bowringiana seedlings were significantly affected when they were transferred from in vitro to ex vitro conditions due to drought stress. Thus, it was revealed that in the early stages of acclimatizing to the altered environments, G. bowringiana seedlings exhibited a rapid increase in photosynthetic pigments, superoxide dismutase activity, and lipid peroxidation levels after being transferred to ex vitro conditions. Comparison of the leaf micromorphologies of G. bowringiana plants grown under in vitro and those grown under ex vitro conditions revealed that leaf development had undergone significant changes during acclimatization to the altered conditions. In vitro to ex vitro transfer leads to a transient decrease in photosynthetic parameters.

show abstract

Acclimatization of the endangered Mexican epiphytic orchid, Laelia speciosa (H.B.K.) Schltr

Cited by 7 publications

References 9 publications

WITHDRAWN: Bio-hardening of in vitro raised plants of Geneva (G.) series clonal rootstocks of apple (Malus X domestica Borkh.)

WITHDRAWN: Bio-hardening of in vitro raised plants of Geneva (G.) series clonal rootstocks of apple (Malus X domestica Borkh.)

Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition

Structural Adaptation and Antioxidant Response of Guarianthe bowringiana (O’Brien) Dressler & W. E. Higgins (Orchidaceae Juss.) Seedlings During Ex Vitro Acclimatization

Contact Info

Product

Resources

About