The object of this research study is to develop a thermal simulation model that can be used to investigate the thermal parameters of the refrigerant systems (with ON-OFF and PID control) used in cooling chambers. Moreover the model was further developed which takes into account also the types of compressors, feeder and control units used commonly in practice for energetic investigations. Using the measured energy consumption data obtained by experimental tests during the previous phase of this research work, the validation of the developed energy simulation model by MatLab R2016a could be also achieved with good agreement. MatLab software seemed to be the most appropriate tool for numerical investigations on the energy consumption of refrigeration systems used in commercial cold stores and transient behavior of the indoor air temperature of the cold store, cooling energy performance and consumed energy to the goods stored in cold store.