Accounting for scattering and reabsorption in the analysis of luminescence spectra of nanoparticles

Kochubey, Vyacheslav I.; Konyukhova, Yu. G.; Zabenkov, I. V.; Волкова, Е. В.

doi:10.1070/qe2011v041n04abeh014596

Cited by 2 publications

(1 citation statement)

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“…The broad emission band of Mn 2+ enhances spectral overlap with the excitation band of the energy acceptor, resulting in an enhanced FRET efficiency [25][26][27][28]. Nonetheless, the reabsorption process, which is unavoidable in homogeneous luminescent bioassays, may interfere with the FRET signal and lead to incorrect detection results [29][30][31]. Therefore, it is of fundamental importance to gain deep insights into the energy transfer process and establish a reliable method for Mn 2+ in homogeneous TR-FRET biodetection.…”

Section: Introductionmentioning

confidence: 99%

Mn2+-activated calcium fluoride nanoprobes for time-resolved photoluminescence biosensing

et al. 2018

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Time-resolved (TR) photoluminescence (PL) technique has shown great promise in ultrasensitive biodetection and high-resolution bioimaging. Hitherto, almost all the TRPL bioprobes are based on the parity-forbidden f→f transition of lanthanide ions. Herein, we report TRPL biosensing by taking advantage of the d→d transition of transition metal (TM) Mn 2+ ion. We demonstrate that the Förster resonance energy transfer (FRET) signal can be distinguished from that of radiative reabsorption process through measuring the PL lifetime of Mn 2+ , thus establishing a reliable method for Mn 2+ in homogeneous TR-FRET biodetection. We also demonstrate the biotin receptor-targeted cancer cell imaging by utilizing biotinylated CaF 2 :Ce,Mn nanoprobes. Furthermore, we show in a proof-of-concept experiment the application of the long-lived PL of Mn 2+ for TRPL bioimaging through the burst shot with a cell phone. These findings provide a general approach for exploiting the long-lived PL of TM ions for TRPL biosensing, thereby opening up a new avenue for the exploration of novel and versatile applications of TM ions.

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