Accumulation and biodistribution of gold nanoparticles in the mesenteric lymph nodes at oral administration

Abstract

Abstract:The aim: to conduct analysis of the accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats conducted with regard to the size and duration of the oral administration. The methods of dark field microscopy with Leica DM 2500 microscope were used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher was used to confirm its identification. Results: It was found that the gold nanoparticles of 1-3 nm were not detected by these methods in any of the areas of the lymph nodes regardless of the duration of administration, gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It was established that the gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes

Influence of the duration of circadian rhythm dis-turbance by light exposure on the morphology of the liver of laboratory rats

“…Experiment duration increase to 21-st days was accompanied with the further damage of the organ parenchyma and circulation failure, that indicated the stress transition to exhaustion stage. The observed results correspond to the data, published earlier, and indicate the development of stress reaction in organism in connection with the long-term light exposure [9,11,18].…”

Bio-functionalized gold nanoparticles: Benign effect in Sprague-Dawley rats by intravenous administration

“…The accumulation and spreading of AuNP in mesenteric lymph nodes was analyzed with respect to size and duration. The 15 and 50 nm gold nanoparticles were detected in the cluster form in the cytoplasm of macrophages and lymphocyte (Zlobina et al, 2013). No toxicity was observed in liver and kidney and concluded its potentiality in obesity related diseases (Chen et al, 2013).…”

Reactivity of stress-implementing system under conditions of light regime change in experiment

“…Severity of changes of physiological functions in organism depends on duration of abnormal exposure, external oscillator power and light regime option. On the works [6][7][8][9][10][11][12] it was demonstrated, that in connection with long-term light exposure the gnawing animals experience reduction of waking hours, degradation of sleep duration and quality, development of functional and structural changes in organs, obesity and type II diabetes mellitus. However, until recently, the issues of activity of the stress-implementing system hormones secretion under conditions of the extended photoperiod, accompanied with alternation of light and dark lighting regimes, remain understudied.…”