Abstract:The aim: to conduct analysis of the accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats conducted with regard to the size and duration of the oral administration. The methods of dark field microscopy with Leica DM 2500 microscope were used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher was used to confirm its identification. Results: It was found that the gold nanoparticles of 1-3 nm were not detected by these methods in any of the areas of the lymph nodes regardless of the duration of administration, gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It was established that the gold nanoparticles of sizes 15 and 50 nm were detected in the form of clusters in the cytoplasm of macrophages and lymphocytes

At present, gold nanoparticles (GNPs) are widely used in biomedical applications such as cancer diagnostics and therapy. Accordingly, the potential toxicity hazards of these nanomaterials and human safety concerns are gaining signi¯cant attention. Here, we report the e®ects of prolonged peroral administration of GNPs with di®erent sizes (2, 15 and 50 nm) on morphological changes in lymphoid organs and indicators of peripheral blood of laboratory animals. The experiment was conducted on 24 white mongrel male rats weighing 180-220 g, gold nanospheres sizes 2, 15 and 50 nm were administered orally for 15 days at a dosage of 190 g/kg of animal body weight. The GNPs were conjugated with polyethylene glycol to increase their biocompatibility and bioavailability. The size-dependent decrease of the number of neutrophils and lymphocytes was noted in the study of peripheral blood, especially pronounced after administration of GNPs with size of 50 nm. The stimulation of myelocytic germ of hematopoiesis was recorded at morphological study of the bone marrow. The signs of strengthening of the processes of di®erentiation and maturation of cellular elements were found in lymph nodes, which were showed as the increasing number of immunoblasts and large lymphocytes. The quantitative changes of cellular component morphology of lymphoid organs due to activation of migration, proliferation and di®erentiation of immune cells indicate the presence of immunostimulation e®ect of GNPs.

Abstract:The influence of gold nanoparticles with different size (1-3 nm, 15 nm and 50 nm) on the morphokinetics of mesenterial lymphatic node cell populations of healthy laboratory animals was investigated. It was established that the oral administration of gold nanoparticles caused the changes of morphokinetics of mesenterial lymphatic node cell populations. The morphological reorganizations in the mesenterial lymphatic nodes testified about activation of migration processes, the proliferation and differentiation processes of immunocompetent cells, which assumes the presence of immunomodulating action of gold nanoparticles.

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