Accumulation of caveolin-3 protein is limited in damaged muscle in chicken muscular dystrophy

Matsumoto, Hirokazu; Sasazaki, Shinji; Fujiwara, Akira; Ichihara, Nobutsune; Kikuchi, Tateki; Mannen, Hideyuki

doi:10.1016/j.cbpa.2010.04.019

Cited by 5 publications

(4 citation statements)

References 47 publications

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“…Our results on masticatory muscles do not differ from those using hindlimb muscles, because in dystrophin-negative soleus muscle, an increase in the cav-3 expression was also observed. The increased abundance of cav-3 in the masticatory muscles and soleus of 100 day old mdx mice is consistent with previous reports demonstrating that cav-3 is upregulated in mdx tibialis anterior [22], in mdx extensor digitorum longus [33], in the muscles of dystrophic chickens [34] and in the quadriceps of DMD patients [21]. However, the mechanisms by which cav-3 is involved in the mdx/DMD dystrophin deficiency remains unclear.…”

Section: Discussionsupporting

confidence: 91%

Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Kunert‐Keil¹,

Gredes²,

Lucke³

et al. 2011

Folia Histochem Cytobiol.

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Section: Discussionsupporting

confidence: 91%

Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Kunert‐Keil¹,

Gredes²,

Lucke³

et al. 2011

Folia Histochem Cytobiol.

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“…The inner surface of caveolae is coated with a scaffolding protein formed by caveolin members [ 53 ]. CAV3 concentration is significantly increased in damaged chicken muscle [ 55 ]. PTRF expression was significantly upregulated after acute stress, and CAV3 expression was significantly upregulated at 0 h of recovery after heat stress ( S2 Table ; Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

Annotation of Differential Gene Expression in Small Yellow Follicles of a Broiler-Type Strain of Taiwan Country Chickens in Response to Acute Heat Stress

Cheng¹,

Tu²,

Wang³

et al. 2015

PLoS ONE

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This study investigated global gene expression in the small yellow follicles (6–8 mm diameter) of broiler-type B strain Taiwan country chickens (TCCs) in response to acute heat stress. Twelve 30-wk-old TCC hens were divided into four groups: control hens maintained at 25°C and hens subjected to 38°C acute heat stress for 2 h without recovery (H2R0), with 2-h recovery (H2R2), and with 6-h recovery (H2R6). Small yellow follicles were collected for RNA isolation and microarray analysis at the end of each time point. Results showed that 69, 51, and 76 genes were upregulated and 58, 15, 56 genes were downregulated after heat treatment of H2R0, H2R2, and H2R6, respectively, using a cutoff value of two-fold or higher. Gene ontology analysis revealed that these differentially expressed genes are associated with the biological processes of cell communication, developmental process, protein metabolic process, immune system process, and response to stimuli. Upregulation of heat shock protein 25, interleukin 6, metallopeptidase 1, and metalloproteinase 13, and downregulation of type II alpha 1 collagen, discoidin domain receptor tyrosine kinase 2, and Kruppel-like factor 2 suggested that acute heat stress induces proteolytic disintegration of the structural matrix and inflamed damage and adaptive responses of gene expression in the follicle cells. These suggestions were validated through gene expression, using quantitative real-time polymerase chain reaction. Functional annotation clarified that interleukin 6-related pathways play a critical role in regulating acute heat stress responses in the small yellow follicles of TCC hens.

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“…Furthermore, the expression of other caveolae-related proteins showed similar tendency as that of caveolin-3. These results suggest that the accumulation of caveolin-3 protein may be associated with the causative process of chicken muscular dystrophy (Matsumoto et al, 2010c). However, more detailed analysis is required in order to elucidate the relationship between WWP1 and caveolin-3.…”

Section: Functional Analysis Toward the Most Likely Candidate Gene Ofmentioning

confidence: 92%

Identification of the Gene Responsible for Chicken Muscular Dystrophy

Matsumoto¹,

Sasazaki²,

Mannen

2011

Korean Journal of Poultry Science

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By a series of positional cloning, we successfully narrowed down the AM candidate region to approximately 1.2 Mbp on GGA2q including 7 functional genes. Subsequently, we identified WWP1 gene as the most likely AM candidate by sequence comparison. The amino acid sequence around the candidate mutation was highly conserved among tetrapods, suggesting that WWP1 is the causative gene of chicken muscular dystrophy. Transfection of mutated WWP1 gene into C2C12 myoblasts disrupted muscle differentiation process. The abnormal muscle differentiation is a characteristic of chicken muscular dystrophy, so we could demonstrate a part of phenotype of the disease. Furthermore, western blotting revealed that accumulation of caveolin-3 protein is limited in damaged muscle of muscular dystrophic chicken, suggesting caveolin-3 may be associated with the pathological change of the disease. We could conclude that WWP1 gene is the responsible one for chicken muscular dystrophy from these results, but the mechanism leading the onset should be clarified in the future. The information will contribute to the study of chicken muscular dystrophy and the corresponding human dystrophies.

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Accumulation of caveolin-3 protein is limited in damaged muscle in chicken muscular dystrophy

Cited by 5 publications

References 47 publications

Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Annotation of Differential Gene Expression in Small Yellow Follicles of a Broiler-Type Strain of Taiwan Country Chickens in Response to Acute Heat Stress

Identification of the Gene Responsible for Chicken Muscular Dystrophy

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