Accumulation of Dehydrin-like-proteins in the Mitochondria of Cold-treated Plants

Боровский, Г. Б.; Stupnikova, I. V.; Antipina, A. I.; Downs, Craig A.; Войников, В. К.

doi:10.1016/s0176-1617(00)80250-3

Cited by 63 publications

(89 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, DHNs were recorded to be localized to plasma membranes during cold acclimation in wheat (Danyluk et al, 1998) and in Thellungiella salsuginea (Rahman et al, 2013). Borovskii et al (2002) found that DHNs can accumulate in mitochondria in response to cold, freezing, and drought in cereals. At the subcellular level, DHNs were detected in the plasma membrane, cytoplasm, and nucleus in Chenopodium quinoa (tribe Atripliceae) (Carjuzaa et al, 2008).…”

Section: Discussionmentioning

confidence: 97%

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

Sadder¹,

Al‐Doss²

2014

Turk J Biol

View full text Add to dashboard Cite

IntroductionAtriplex spp. (saltbush) is a xerohalophyte that belongs to the subfamily Chenopodiaceae and family Amaranthaceae (http://www.mobot.org/MOBOT/research/APweb/). Saltbush is able to produce huge biomass (Glenn et al., 1999). In addition, it has been utilized as livestock fodder with good protein content (Khan et al., 2000). Natural populations of the Mediterranean saltbush (A. halimus L.) are distributed in subhumid and arid regions including North Africa and the Arabian Peninsula (Le Houerou, 1992;Al-Turki et al., 2000; http://ww2.bgbm. org/herbarium/). They have evolved many adaptation mechanisms and physiological responses to cope with different abiotic stresses (Sadder et al., 2013).Water deficit is a major stress caused by limited water availability in soil; however, it can also be induced by salt stress. Generated stress signals usually activate controlling pathways in plants for ionic/osmotic homeostasis and detoxification (Rodríguez-Milla and Salinas, 2009;Li et al., 2013). Dehydrins (DHNs) are plant proteins that belong to the "late embryogenesis abundant II" group. They play a fundamental role in plant response and adaptation to multiple abiotic stresses (Hanin et al., 2011). DHNs were found to be associated with drought stress in olives (Tripepi et al., 2011), with osmotic stress in tea (Paul and Kumar, 2013) and with salt stress in Physcomitrella patens (Ruibal et al., 1012) and tomatoes (Muñoz-Mayor et al., 2012). In addition, they were found associated with cold stress in soybean (Yamasaki et al., 2013) and spinach (Chen et al., 2012).DHNs contain several hydrophilic residues. Any reduction in hydration or increase in compatible solutes can lead to conformational and functional changes (Danyluk et al., 1998;Hanin et al., 2011). DHN in α-helical conformation displays amphipathic protein-protein or protein-membrane interactions. Therefore, DHN can protect other proteins from further loss of water envelope (Koag et al., 2003). Furthermore, DHNs can elevate tolerance through radical-reducing activity, where H, R, and other reactive residues on their surfaces exhibit reactive oxygen species (ROS) scavenging ability (Hanin et al., 2011). The interactions of a residue with ROS can lead to oxidation of the residue, and thus DHNs can function as antioxidants (Hara et al., 2013). Moreover, DHNs can maintain cellular structure by acting as space-fillers between intracellular complexes (Tunnacliffe and Wise, 2007). This study was carried out to clone and characterize a novel DHN gene from A. halimus.

show abstract

Section: Discussionmentioning

confidence: 97%

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

Sadder¹,

Al‐Doss²

2014

Turk J Biol

View full text Add to dashboard Cite

show abstract

“…DHNs localize primarily to the nucleus or cytoplasm (Houde et al, 1995;Bracale et al, 1997), but studies also corroborate their presence either in the plasma membrane (Danyluk et al, 1998), endoplasmic reticulum (Neven et al, 1993), chloroplast (Wisniewski et al, 1999), mitochondria (Borovskii et al, 2002) or even in the vacuole (Heyen et al, 2002). DHNs may thus be expressed either constitutively [e.g.…”

Section: Structure and Functional Studies Of Dhnamentioning

confidence: 87%

“…Localization of specific dehydrins at the plasma membrane (Danyluk et al 1998) and also on the endomembranes (Dure, 1993) is consistent with their role in stabilizing cellular structures during dehydration stress (Kazuoka and Oeda, 1994;Close, 1997;Nylander et al, 2001). Dehydrin-like proteins (DLPS) are known to accumulate in organelles such as mitochondria of Triticum aestivum, Secale cereale and in also in Zea mays in response to drought, freezing, cold, and exogenous ABA treatment (Borovskii et al, 2002). These group 2 LEA proteins have been further categorized under POPPs classification, into two subgroups: group 2a, that are not specifically induced under cold stress along with expression during late embryogenesis and group 2b LEA proteins, that show cold tolerance and are explicitly not produced during late embryogenesis (Wise and Tunnacliffe, 2004).…”

Section: Group 2 Lea Protein/dehydrins (Dhns)mentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

show abstract

“…Some dehydrins also accumulated in the vicinity of plasma membrane and were suggested to inhibit lipid peroxidation caused by ROS (Danyluk et al 1998). In the last a few years, dehydrins have been proved to be distributed in mitochondria and chloroplast (Borovskii et al 2000;Mueller et al 2003), which are known to be important ROS-generating places and susceptible to the attack of free radicals (Salin 1991). These results argued that the function of dehydrins is associated with ROS or dehydrins may play an antioxidative role in plants under environmental stresses.…”

Section: Many Studies Indicated That Plant Dehydrins Have Antioxidatimentioning

confidence: 98%

Role of plant dehydrins in antioxidation mechanisms

Sun

Lin

2010

Biologia

View full text Add to dashboard Cite

Plant antioxidation system is composed of a series of complex mechanisms, in which many antioxidants including some special proteins are involved. Dehydrins are a family of late embryogenesis abundant (LEA) proteins which usually accumulate in plants during late embryogenesis or in response to environmental stresses. They were suggested to be associated with specific protective functions in plant cells, such as preventing coagulation of macromolecules and maintaining integrity of crucial cell structures. In recent years, many studies implied that dehydrins also play an antioxidative role to alleviate oxidative damage in stressed plants. They were proposed to scavenge radicals directly and sequester metals which are sources for radical generation to avoid the production of reactive oxygen species (ROS). In this paper, we will discuss the novel putative role of dehydrins in plant antioxidation mechanisms and how dehydrins perform their antioxidative activity.

show abstract

Accumulation of Dehydrin-like-proteins in the Mitochondria of Cold-treated Plants

Cited by 63 publications

References 22 publications

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

Characterization of dehydrin AhDHN from Mediterranean saltbush (Atriplex halimus)

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Role of plant dehydrins in antioxidation mechanisms

Contact Info

Product

Resources

About