Accumulation of Dimethylpyruvic Acid in Biotin-Deficient Culture of Piricularia Oryzae

Katsuki, Hirohiko

doi:10.1021/ja01622a084

Cited by 4 publications

(2 citation statements)

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“…Nicotinic acid occurs in living cells in the form of pyridine nucleotides, DPN and TPN, and accumulation in the culture A. H. ROSE medium of nicotinic acid and desamido-DPN suggests that synthesis of pyridine nucleotides by Succharomyces cerevisiae is impaired under conditions of biotin deficiency. A deficiency of pyridine nucleotides in the biotin-deficient cells was not demonstrated experimentally in the present work, although this situation has been reported with Pirimlaria oryxae (Katsuki, 1959 a) and with Bacillus mucerans (Katsuki, 1959b).…”

Section: Discussioncontrasting

confidence: 50%

“…Among the intermediates which have been detected are an aromatic amine, probably 5-amino-imidazole riboside (Chamberlain, Cutts & Rainbow, 1952 Friedman, 1956) and hypoxanthine (Chamberlain & Rainbow, 1954), excretion of both these compounds being the result of an impairment in the ability of biotindeficient micro-organisms to synthesize purines. It has also been reported that under conditions of biotin deficiency, pyruvate, dimethyl-pyruvate and a-ketoglutarate are excreted by Pirkularia oryzue (Katsuki, 1959 a), while pyruvate can also be detected in filtrates from biotin-deficient cultures of BaciZZus macerans (Katsuki , 1959 b).…”

Section: Introductionmentioning

confidence: 98%

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Excretion of Nicotinic Acid by Biotin-Deficient Saccharomyces cerevisiae

Rose

1960

Journal of General Microbiology

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SUMMARYGrowth of a strain of Sacchrmyces ce~evisiae in a glucose salts vitamins medium, containing suboptimal concentrations of biotin, was accompanied by the appearance in the medium of a free form of nicotinic acid, and also a heat-labile combined form of this substance; these were identified chromatographically as nicotinic acid and dessmido-DPN (nicotinic acid adenine dinucleotide), respectively. The effect of biotin concentration and incubation time on the excretion of these substances is reported. The presence of adenine in the growth medium caused a marked decrease in the excretion of both forms of nicotinic acid, but hypoxanthine and &amino-5-imidazole carboxamide had no observable effect. Addition of DL-aspartic acid to the medium also brought about a decrease in the amounts excreted, although concentrations of this amino acid in excess of 0.5 x 10%~ stimulated excretion of desamido-DPN. It is concluded that excretion of free nicotinic and desamido-DPN is largely the result of an impairment in the ability of the biotin-deficient yeast to produce adenine, which in turn leads to a derangement in the synthesis of pyridine nucleotides.

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Section: Discussioncontrasting

confidence: 50%

Section: Introductionmentioning

confidence: 98%

Excretion of Nicotinic Acid by Biotin-Deficient Saccharomyces cerevisiae

Rose

1960

Journal of General Microbiology

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α‐Keto Acid Determinations

Neish

1957

Methods of Biochemical Analysis

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Interaction between dimethylpyruvic acid and biotin with a mould

Brouwer

Ylstra

Berends

1959

Recl. Trav. Chim. Pays‐Bas

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The separation of dimethylpyruvic acid observed by Katsuki from the mould, Piricularia oryzae, with a deficiency of biotin, has been investigated. The formation of dimethylpyruvic acid does not depend on aeration. Addition of biotin leads to the absorption of dimethylpyruvic acid so that there is possibly a connection between the separation of this acid and the function of biotin. A method for the determination of dimethylpyruvic acid in the presence of pyruvic acid and α‐ketoglutaric acid has been worked out.

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Accumulation of Dimethylpyruvic Acid in Biotin-Deficient Culture of Piricularia Oryzae

Cited by 4 publications

References 1 publication

Excretion of Nicotinic Acid by Biotin-Deficient Saccharomyces cerevisiae

Excretion of Nicotinic Acid by Biotin-Deficient Saccharomyces cerevisiae

α‐Keto Acid Determinations

Interaction between dimethylpyruvic acid and biotin with a mould

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