Accumulation of Flavonoid Glycosides and <scp>UFGT</scp> Gene Expression in Mulberry Leaves (<i>Morus alba</i> L.) before and after Frost

Yu, Xin; Zhu, Yuan; Fan, Jingyi; Wang, Dujun; Gong, Xiaomeng; Ouyang, Zhen

doi:10.1002/cbdv.201600496

Cited by 23 publications

(15 citation statements)

References 27 publications

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“…Post-transcriptional or post-translational regulation of UFGT has been proposed by several authors in the last years. In a recent research conducted on mulberry leaves 64 a correlation analysis was conducted among the expression level of UFGT, UFGT activity and flavonoid glycosides accumulation and it was shown that UFGT activity is much more correlated than UFGT expression with flavonoid glucosides accumulation. A similar behaviour was also recorded in grapevine under high 41 , 50 or low night 21 temperatures and we can hypothesise that in our study the activity of UFGT for the entire duration of TV treatment could be inhibited by low night temperature (around 10 °C) in comparison with ambient night temperature (15–20 °C).…”

Section: Discussionmentioning

confidence: 99%

Low night temperature at veraison enhances the accumulation of anthocyanins in Corvina grapes (Vitis Vinifera L.)

Gaiotti

Pastore

Filippetti

et al. 2018

Sci Rep

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Climate change is a major concern in grape production worldwide. Nights have been warming much faster than the days, raising attention on the effect of night temperatures on grape and wine composition. In this study we evaluated the effect of night temperatures on grape coloration in the cv. Corvina (Vitis vinifera L.). In 2015 and 2016 potted plants were cooled overnight (10–11 °C) during two berry ripening phases, veraison (TV) or post-veraison (TPV), and compared to control vines (C) grown at ambient night temperature (15–20 °C on average). Cooling treatment around veraison (TV) hastened berry anthocyanin accumulation, while the same treatment applied after veraison (TPV) was ineffective. Molecular analysis revealed an increased transcription of four key genes in anthocyanin biosynthesis (CHS3, F3H1, MYBA1 and UFGT) in TV treatment. These results suggest that the anthocyanin biosynthesis capacity was enhanced by cool nights during veraison. However, since the gene expression was not always temporally correlated to the increase in anthocyanin concentration, we speculate on the presence of mechanisms, such as enzymatic regulation or anthocyanin transport, which may contribute in determining the anthocyanin accumulation under low night temperatures.

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Section: Discussionmentioning

confidence: 99%

Low night temperature at veraison enhances the accumulation of anthocyanins in Corvina grapes (Vitis Vinifera L.)

Gaiotti

Pastore

Filippetti

et al. 2018

Sci Rep

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“…Low temperature-induced anthocyanin accumulation in leaves and stems of Arabidopsis thaliana and facilitated anthocyanin synthesis through the phenylpropanoid pathway associated with increased transcripts of flavonoid biosynthetic genes including PAL and CHS ( Leyva et al, 1995 ). The expression of the UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT) in mulberry leaves could be induced by low temperature and resulted in the accumulation of flavonoid glycosides ( Yu et al, 2017 ). Thus, we hypothesized that flavonoid synthesis in mulberry leaves increases under conditions of low temperature, as a stress response to resist chilling.…”

Section: Introductionmentioning

confidence: 99%

Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times

Cheng

Zhang

et al. 2021

Front. Plant Sci.

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The mulberry leaf is a classic herb commonly used in traditional Chinese medicine. It has also been used as animal feed for livestock and its fruits have been made into a variety of food products. Traditionally, mulberry (Morus alba L.) leaf harvesting after frost is thought to have better medicinal properties, but the underlying mechanism remains largely unsolved. To elucidate the biological basis of mulberry leaves after frost, we first explored the content changes of various compounds in mulberry leaves at different harvest times. Significant enrichment of flavonoids was observed with a total of 224 differential metabolites after frost. Subsequently, we analyzed the transcriptomic data of mulberry leaves collected at different harvest times and successfully annotated 22,939 unigenes containing 1,695 new genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed 26, 20, and 59 unigenes related to flavonoids synthesis in three different groups harvested at different times. We found that the expression levels of flavonoid biosynthesis-related unigenes also increased when harvested at a delayed time, which was consistent with the flavonoid accumulation discovered by the metabolomic analysis. The results indicated that low temperature may be a key trigger in flavonoid biosynthesis of mulberry leaves by increasing the expression of flavonoid biosynthesis-related genes. This study also provided a theoretical basis for the optimal harvest time of mulberry leaves.

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“…In tea plants, low-temperature treatment induced high CsHCT transcription, resulting in the production of chlorogenic acid or acylated flavonol glycosides 29 . In mulberry leaves, low temperatures could also promote the expression of the UFGT gene and lead to flavonoid accumulation 30 . However, the function of McMYB4 in flavonol biosynthesis pathways in response to temperature changes is unclear.…”

Section: Discussionmentioning

confidence: 99%

McMYB4 improves temperature adaptation by regulating phenylpropanoid metabolism and hormone signaling in apple

Hao

Peng

et al. 2021

Hortic Res

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Temperature changes affect apple development and production. Phenylpropanoid metabolism and hormone signaling play a crucial role in regulating apple growth and development in response to temperature changes. Here, we found that McMYB4 is induced by treatment at 28 °C and 18 °C, and McMYB4 overexpression results in flavonol and lignin accumulation in apple leaves. Yeast one-hybrid (Y1H) assays and electrophoretic mobility shift assays (EMSAs) further revealed that McMYB4 targets the promoters of the flavonol biosynthesis genes CHS and FLS and the lignin biosynthesis genes CAD and F5H. McMYB4 expression resulted in higher levels of flavonol and lignin biosynthesis in apple during growth at 28 °C and 18 °C than during growth at 23 °C. At 28 °C and 18 °C, McMYB4 also binds to the AUX/ARF and BRI/BIN promoters to activate gene expression, resulting in acceleration of the auxin and brassinolide signaling pathways. Taken together, our results demonstrate that McMYB4 promotes flavonol biosynthesis and brassinolide signaling, which decreases ROS contents to improve plant resistance and promotes lignin biosynthesis and auxin signaling to regulate plant growth. This study suggests that McMYB4 participates in the abiotic resistance and growth of apple in response to temperature changes by regulating phenylpropanoid metabolism and hormone signaling.

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Accumulation of Flavonoid Glycosides and UFGT Gene Expression in Mulberry Leaves (Morus alba L.) before and after Frost

Cited by 23 publications

References 27 publications

Low night temperature at veraison enhances the accumulation of anthocyanins in Corvina grapes (Vitis Vinifera L.)

Low night temperature at veraison enhances the accumulation of anthocyanins in Corvina grapes (Vitis Vinifera L.)

Morus alba L. Leaves – Integration of Their Transcriptome and Metabolomics Dataset: Investigating Potential Genes Involved in Flavonoid Biosynthesis at Different Harvest Times

McMYB4 improves temperature adaptation by regulating phenylpropanoid metabolism and hormone signaling in apple

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