Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection

Showalter, Allan M.; Bell, John; Cramer, Carole L.; Bailey, J. A.; Varner, Joseph E.; Lamb, Chris

doi:10.1073/pnas.82.19.6551

Cited by 247 publications

(140 citation statements)

References 45 publications

Supporting

Mentioning

125

Contrasting

Unclassified

Order By: Relevance

“…This oxidative burst may kill the pathogen directly (Keppler et al, 1989;Wu et al, 1995) or limit its spread by killing infected plant cells (Greenberg et al, 1994) and inducing cross-linkage of cell wall proteins (Bradley et al, 1992;Brisson et al, 1994). During an incompatible interaction, cell walls are also strengthened through increased deposition of hydroxy-Pro-rich glycoproteins (Showalter et al, 1985), callose (Parker et al, 1993), and lignin (Moerschbacher et al, 1990). Infection with an avirulent pathogen often causes a stronger and more rapid increase in pathogenesis-related (PR) proteins (Linthorst, 1991), which may enhance resistance to fungi (Broglie et al, 1991;Zhu et al, 1994).…”

mentioning

confidence: 99%

Response to Xanthomonas campestris pv.vesicatoria in Tomato Involves Regulation of Ethylene Receptor Gene Expression

et al. 2000

View full text Add to dashboard Cite

Although ethylene regulates a wide range of defense-related genes, its role in plant defense varies greatly among different plant-microbe interactions. We compared ethylene's role in plant response to virulent and avirulent strains of Xanthomonas campestris pv. vesicatoria in tomato (Lycopersicon esculentum Mill.). The ethylene-insensitive Never ripe (Nr) mutant displays increased tolerance to the virulent strain, while maintaining resistance to the avirulent strain. Expression of the ethylene receptor genes NR and LeETR4 was induced by infection with both virulent and avirulent strains; however, the induction of LeETR4 expression by the avirulent strain was blocked in the Nr mutant. To determine whether ethylene receptor levels affect symptom development, transgenic plants overexpressing a wild-type NR cDNA were infected with virulent X. campestris pv. vesicatoria. Like the Nr mutant, the NR overexpressors displayed greatly reduced necrosis in response to this pathogen. NR overexpression also reduced ethylene sensitivity in seedlings and mature plants, indicating that, like LeETR4, this receptor is a negative regulator of ethylene response. Therefore, pathogen-induced increases in ethylene receptors may limit the spread of necrosis by reducing ethylene sensitivity.

show abstract

mentioning

confidence: 99%

Response to Xanthomonas campestris pv.vesicatoria in Tomato Involves Regulation of Ethylene Receptor Gene Expression

et al. 2000

View full text Add to dashboard Cite

show abstract

“…The bean enzyme, which is co-induced with microsomal arabinosyl transferase and hydroxyproline-rich glycoprotein [3,4,47], is located within the smooth endoplasmic reticulum, requires oxoglutarate, Fe2+ and ascorbate and has a subunit M , of 65000 [21]. The polyclonal antiserum raised in rabbits against the Mr-65000 polypeptide here described binds to the enzyme subunit with high titre, is capable of precipitation of solubilized enzyme and immunoprecipitates an M1-65 000 polypeptide from microsomal proteins newly synthesized in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, increased levels of mRNA encoding extensin have been detected following elicitation [4]. These studies have involved the production of antibody and cDNA probes.…”

Section: 2-)mentioning

confidence: 99%

“…Chalcone isomerase (EC 5.5.1.6); chalcone synthase, cinnamic acid 4-hydroxylase or trans-cinnamate 4-monooxygenase (EC 1.14.13.11); L-phenylalanine ammonia-lyase (EC 4.3.1.5); proline 2-oxoglutarate 4-dioxygenase or prolyl hydroxylase (EC 1.14.1 1.2); protein arabinosyltransferase (EC 2. 4…”

mentioning

confidence: 99%

“…Modulation of activities involved in a number of these processes has been described in endomembranes isolated from cell-suspension cultures of dwarf French bean (Phaseolus vulguris L.) exposed to an elicitor preparation from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthiunum. Prominent among these are two oxygenase activities, the cytochrome-P-450-dependent hydroxylation of cinnamic acid involved in the production of phenylpropanoid precursors of antifungal isoflavonoid phytoalexins and wall-bound phenolic compounds [I -31, and the hydroxylation of peptide proline during the post-translational modification of arabinosylated-hydroxyproline-rich glycoprotein and extensin, both of which are induced in response to elicitor treatment [3,4]. …”

mentioning

confidence: 99%

See 2 more Smart Citations

Membrane‐bound hydroxylases in elicitor‐treated bean cells

Bolwell

Dixon

1986

European Journal of Biochemistry

View full text Add to dashboard Cite

1. Treatment of cell-suspension cultures of bean (Phaseolus vulguris cv. Canadian Wonder) with an elicitor preparation heat-released from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthianum resulted in rapid changes in the activities of two microsomal oxygenases, cinnamic acid 4-hydroxylase7 involved in accumulation of wall-bound phenolics and phytoalexins, and proline 2-oxoglutarate dioxygenase (prolyl hydroxylase) involved in the post-translational modification of hydroxyproline-rich glycoproteins.2. An anti-(cytochrome P-450) monoclonal antibody, originally raised against rat cytochrome P-450 isoform c, has been shown to bind to bean microsomes and recognise in Western blots an M,-48000 polypeptide, which comigrates with a haeme-containing protein on SDS/polyacrylamide gel analysis and which has been tentatively identified as a cytochrome P-450 capable of the hydroxylation of cinnamic acid.3. A preparation of proline 2-oxoglutarate dioxygenase purified to homogeneity was used to immunise rabbits for the production of antiserum. An elicitor-induced polypeptide of MI 65000 was identified as prolyl hydroxylase while an antigenically related polypeptide of M I 60000 was also immunoprecipitated but not induced by elicitor treatment.4. Use of the two antibodies has demonstrated rapid transient increases in the synthesis of the M , 48000 and M I 65000 oxygenases in vivo and for mRNAs as measured in in vitro translations, particularly for the putative cytochrome P-450. These increases slightly precede corresponding changes in the synthesis of the soluble enzyme phenylalanine ammonia-lyase, in common with which these oxygenases probably share a mechanism of gene activation underlying the increased activities seen in response to elicitor treatment.Plant endomembrane systems mediate the synthesis, modification and export of wall-bound materials. Among these functions, synthesis of matrix polysaccharides, posttranslational modification and glycosylation of proteins and aromatic hydroxylation of phenolic precursors have particularly been identified. Modulation of activities involved in a number of these processes has been described in endomembranes isolated from cell-suspension cultures of dwarf French bean (Phaseolus vulguris L.) exposed to an elicitor preparation from the cell walls of the phytopathogenic fungus Colletotrichum lindemuthiunum. Prominent among these are two oxygenase activities, the cytochrome-P-450-dependent hydroxylation of cinnamic acid involved in the production of phenylpropanoid precursors of antifungal isoflavonoid phytoalexins and wall-bound phenolic compounds [I -31, and the hydroxylation of peptide proline during the post-translational modification of arabinosylated-hydroxyproline-rich glycoprotein and extensin, both of which are induced in response to elicitor treatment [3,4].

show abstract

What Defenses Do Plants Use?

Walters

2010

Plant Defense

View full text Add to dashboard Cite

What are defenses in plants and how do they help or hinder the plant?What do plant defenses entail? Plants, like all organisms, can evolve over time. How and when a plant evolves depends on the surrounding environment. Those fit to survive in that environment possess the biological make-up to live, and those not fit dwindle away along with their unfit genes. This is known as natural selection, a concept introduced by Charles Darwin. Afterwards, when the plants in that area mate, the fit genes will be more prominent. Mutations in plants also bring about evolution. Plants have the means to protect themselves from outside factors that may harm them. These mechanisms come in two different forms, constitutive and induced responses. Constitutive responses are always present in the plant, while induced are only produced as a reaction. The ways in which pathogens and other organisms have attacked plants has evolved over the years, but so has the plants way in which it fights off these intruders. All plants deal with intruders in different ways, and some in similar ways. Most ways benefit the plant, but not all do. For example, when a

show abstract

Accumulation of hydroxyproline-rich glycoprotein mRNAs in response to fungal elicitor and infection

Cited by 247 publications

References 45 publications

Response to Xanthomonas campestris pv.vesicatoria in Tomato Involves Regulation of Ethylene Receptor Gene Expression

Response to Xanthomonas campestris pv.vesicatoria in Tomato Involves Regulation of Ethylene Receptor Gene Expression

Membrane‐bound hydroxylases in elicitor‐treated bean cells

What Defenses Do Plants Use?

Contact Info

Product

Resources

About