Accumulation of Rutin and Betulinic Acid and Expression of Phenylpropanoid and Triterpenoid Biosynthetic Genes in Mulberry (<i>Morus alba</i>L.)

Zhao, Shicheng; Park, Chang Ha; Li, Xiaohua; Kim, Yeon Bok; Yang, Jingli; Sung, Gyoo Byung; Park, Nam Il; Kim, Soonok; Park, Sang Un

doi:10.1021/acs.jafc.5b03221

Cited by 44 publications

(53 citation statements)

References 35 publications

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“…After the initial screening by docking and fluorescence studies, role of two natural compounds rutin and vanillin as an inhibitor of MARK4 has been studied in detail. Rutin is a dietary flavonoid and vanillin is a phenolic aldehyde, both are present in our routine foodstuffs like citrus fruits and vegetables 22 , 57 , 59 . Here we investigate their role in MARK4 inhibition.…”

Section: Discussionmentioning

confidence: 99%

Elucidation of Dietary Polyphenolics as Potential Inhibitor of Microtubule Affinity Regulating Kinase 4: In silico and In vitro Studies

Khan

Rahman

Queen

et al. 2017

Sci Rep

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Microtubule affinity regulating kinase 4 (MARK4) is a Ser/Thr kinase belonging to AMPK-like family, has recently become an important drug target against cancer and neurodegenerative disorders. In this study, we have evaluated different natural dietary polyphenolics including rutin, quercetin, ferulic acid, hesperidin, gallic acid and vanillin as MARK4 inhibitors. All compounds are primarily binds to the active site cavity of MARK4. In silico observations were further complemented by the fluorescence-binding studies and isothermal titration calorimetry (ITC) measurements. We found that rutin and vanillin bind to MARK4 with a reasonably high affinity. ATPase and tau-phosphorylation assay further suggesting that rutin and vanillin inhibit the enzyme activity of MARK4 to a great extent. Cell proliferation, ROS quantification and Annexin-V staining studies are clearly providing sufficient evidences for the apoptotic potential of rutin and vanillin. In conclusion, rutin and vanillin may be considered as potential inhibitors for MARK4 and further exploited to design novel therapeutic molecules against MARK4 associated diseases.

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Section: Discussionmentioning

confidence: 99%

Elucidation of Dietary Polyphenolics as Potential Inhibitor of Microtubule Affinity Regulating Kinase 4: In silico and In vitro Studies

Khan

Rahman

Queen

et al. 2017

Sci Rep

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“…Amino acid sequence analysis of these two clones indicated a high level of homology (more than 80%) to known CHS family proteins isolated from other plant species ( Figure 4). Accordingly, Unigene15633 and CL3598 are designated as MaCHS4 and MaCHS5 respectively, because three other CHS genes (MaCHS1, MaCHS2, MaCHS3) have been described previously (Zhao et al, 2015).…”

Section: Cloning Genes Encoding Key Flavonoid Biosynthesis Enzymesmentioning

confidence: 99%

Possible involvement of flavonoids in response of mulberry (Morus alba L.) to infection with Ralstonia solanacearum (Smith 1896) Yabuuchi et al., 1996

Dai¹,

Luo²,

Wang³

et al. 2019

European.J.Hortic.Sci

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Bacterial wilt in mulberry (Morus atropurpurea), caused by the phytopathogenic bacteria Ralstonia solanacearum, is one of the most important production constraints in many tropical and subtropical areas. In our previous work, we have investigated gene expression changes in mulberry at the early stages of R. solanacearum infection. In this work we have investigated transcriptional changes in mulberry that occur during all infection process caused by R. solanacearum in mulberry. Analysis of gene expression changes in two mulberry cultivars, following infection with R. solanacearum, revealed that expression of a series of genes associated with flavonoid biosynthesis was significantly involved into infection at all treated time points. Specifically, the expression of genes encoding the key-enzymes involved in flavonoid biosynthesis-such as chalcone synthase, chalcone isomerase, and flavonoid 3'-hydroxylase-were altered, and most of these genes were regulated more significantly in resistant cultivar KQ10 than in susceptible cultivar YSD10 after infection with R. solanacearum. We obtained fulllength sequences of these three genes and additional transcriptional and phytochemical evidence to verify this result using qPCR and aluminum chloride colorimetry. This work indicates that flavonoids may play important roles in bacterial wilt resistance for mulberry plants and provides valuable gene targets for breeding disease-resistant mulberry cultivars.

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“…The above results showed that the UFGT gene was the key factor in isoquercitrin and astragalin biosynthesis in mulberry leaves, but not the key factor of rutin biosynthesis. Zhao et al [23] reported that flavonol-3-O-glucoside L-rhamnosyltransferase (UGT78D1) plays an important regulatory role in the rutin biosynthetic pathways in mulberry leaves (Morus alba L.), but UFGT is not the key enzyme. This conclusion can explain why the UFGT gene is not the key factor of rutin biosynthesis in mulberry leaves.…”

Section: Correlation Analysis Between Average Temperature and Expressmentioning

confidence: 99%

Accumulation of Flavonoid Glycosides and UFGT Gene Expression in Mulberry Leaves (Morus alba L.) before and after Frost

Zhu

Fan

et al. 2017

Chemistry & Biodiversity

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In order to determine the molecular mechanism underlying the influence of frost on chemical changes in mulberry leaves, the UFGT activity, expression level, and accumulation of flavonoid glycosides in mulberry leaves (Morus alba L.) were studied. The expression of UFGT gene was investigated by quantitative real-time PCR (qRT-PCR) and the UFGT activity, accumulation of flavonoid glycosides was studied by high performance liquid chromatography. Then, the correlation between the expression level of UFGT, the UFGT activity, and the flavonoid glycosides accumulation with temperature was explored. The accumulation of isoquercitrin and astragalin is significantly positively correlated with UFGT gene expression and UFGT activity. On the contrary, the average temperature was significantly negatively correlated with the level of UFGT gene expression and UFGT activity. The results show that after frost, low temperature can induce the expression of UFGT gene in mulberry leaves, resulting in the accumulation of flavonoid glycosides.

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Accumulation of Rutin and Betulinic Acid and Expression of Phenylpropanoid and Triterpenoid Biosynthetic Genes in Mulberry (Morus albaL.)

Cited by 44 publications

References 35 publications

Elucidation of Dietary Polyphenolics as Potential Inhibitor of Microtubule Affinity Regulating Kinase 4: In silico and In vitro Studies

Elucidation of Dietary Polyphenolics as Potential Inhibitor of Microtubule Affinity Regulating Kinase 4: In silico and In vitro Studies

Possible involvement of flavonoids in response of mulberry (Morus alba L.) to infection with Ralstonia solanacearum (Smith 1896) Yabuuchi et al., 1996

Accumulation of Flavonoid Glycosides and UFGT Gene Expression in Mulberry Leaves (Morus alba L.) before and after Frost

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