Accumulation of SALT protein in rice plants as a response to environmental stresses

Filho, Gonçalo A. de Souza; Ferreira, Beatriz S.; Dias, Janice; Queiroz, Karlla S.; Branco, Alan T.; Bressan-Smith, Ricardo; Oliveira, Jurandi Gonçalves de; Garcia, Anice

doi:10.1016/s0168-9452(03)00014-1

Cited by 71 publications

(37 citation statements)

References 26 publications

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“…It was clear that TPP1 expression was induced earlier and to a higher level by cold conditions, whereas SalT expression was inducible by salinity stress to a much greater extent than by cold ( Figure 5). These results agree with the expression behavior of the two genes reported in the literature [35,38]. For testing the use of EP in indica type rice, we used the IR20 and NSG19 cultivars and the jasmine rice cultivar KDML105.…”

Section: Qpcr With Ep As the Reference Reveals Different Expression Psupporting

confidence: 88%

High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

2013

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Rice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm PLUS and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.

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Section: Qpcr With Ep As the Reference Reveals Different Expression Psupporting

confidence: 88%

High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

2013

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“…Salt-induced proteins in rice leaf sheaths were also observed by several authors 4,10,26 . de Souza Filho et al 4 demonstrated that SALT protein (14.5 kDa) expression is induced in roots, sheaths and laminae of rice plants exposed to 170 mM NaCl. Claes et al 10 reported that salinity induced changes in 15 kDa protein synthesis are often more pronounced in both leaf sheaths and in roots of salt-sensitive rice cv.…”

Section: Resultssupporting

confidence: 62%

“…There are many reports concerning the correlation between salt protein levels and the level of salinity tolerance using the physiological parameters such as photochemical efficiency of photosynthesis and net photosynthesis rate 4,27 . Moreover, Na + /K + ratio and chlorophyll content can be used as an indication of the stress tolerance capacity in rice and used for screening salt-tolerant lines of rice 28,29 .…”

Section: Resultsmentioning

confidence: 99%

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Kong-ngern¹,

Daduang²,

Wongkham³

et al. 2005

ScienceAsia

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Salt-induced changes in protein synthesis in leaf sheaths of the Thai rice cv. Leaung Anan were investigated using one-dimensional SDS-PAGE and two-dimensional PAGE (2DE). In SDS-PAGE, two protein bands (22 and 31 kDa) whose expression was specifically increased under salt stress were identified. Enhanced expression of these protein bands was consistently observed in plants grown under greenhouse conditions saline during different time of the year. In 2DE, at least thirteen different proteins were induced and ten different proteins were decreased by salt. Western blot analysis of the 31 kDa of rice seedlings of three cultivars: salt-tolerant Pokkali, moderately tolerant Leuang Anan, and salt-sensitive KDML 105, showed different expression patterns. The protein was strongly induced by salinity in leaf sheaths of treated tolerant Pokkali and Leuang Anan, compared to control plants. In contrast, its level in the sensitive KDML 105 remained unchanged with salt treatment. Moreover, rice cv. Leaung Anan showed higher expression of the 31 kDa protein than Pokkali. These results suggest that the 31 kDa polypeptide is a salt-induced protein in rice leaf sheaths.

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“…A salt-inducible rice gene SalT which was isolated from salt-stressed rice (Claes et al 1990;Peumans et al 2000) encoded a jacalin-related lectin . This rice lectin was not detected in untreated plants while detected in roots subjected to salt, drought and heat stress, or treated with jasmonic acid and abscisic acid (Garcia et al 1998;de Souza et al 2003). Horcolin (H. vulgare coleoptile lectin), a jacalin-related lectin from barley coleoptiles (Grunwald et al 2007) was exclusively expressed in both light-or dark-grown coleoptiles but not in leaves or roots, and also up-regulated under salt stress.…”

Section: Discussionmentioning

confidence: 98%

Isolation of a low-sulfur tolerance gene from Eichhornia crassipes using a functional gene-mining approach

Liu

Chen

Oliver

et al. 2009

Planta

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Genes enhancing nutrient utilization efficiency are needed for crop improvement. Here, we report the isolation of a gene conferring low-sulfur tolerance from water hyacinth (Eichhornia crassipes) using a functional gene-mining method. In doing this, an entry cDNA library was constructed from the roots of nutrient-starved water hyacinth using recombination cloning and subsequently shuttled into the plant transformation- and expression-ready vector. The plant transformation- and expression-ready library was transferred into Arabidopsis and a seed library of 50,000 independent transgenic lines was generated. Three transgenic lines with enhanced low-sulfur tolerance were isolated from the seed library. One of the transgenic lines, shl143-1, with improved tolerance to sulfate deficiency and an improved root system was further analyzed. It was found that a water hyacinth jacalin-related lectin gene (EcJRL-1) was overexpressed in shl143-1. Recapitulation analysis confirmed that the overexpression of the EcJRL-1 cDNA caused the phenotype. Therefore, this study demonstrates that a jacalin-related lectin is involved in root elongation under sulfur-deficient conditions.

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Accumulation of SALT protein in rice plants as a response to environmental stresses

Cited by 71 publications

References 26 publications

High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

Untitled

Isolation of a low-sulfur tolerance gene from Eichhornia crassipes using a functional gene-mining approach

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