Accumulation of sweet protein monellin is regulated by thepsbA 5′UTR in tobacco chloroplasts

Roh, Kyung Hee; Shin, Kong-Sik; Lee, Yeon-Hee; Seo, Seok-Cheol; HyoGuen, Park; Daniell, Henry; Lee, Seung Bum

doi:10.1007/bf03030786

Cited by 20 publications

(7 citation statements)

References 63 publications

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“…Therefore, overexpression and production of monellin primarily by molecular pharming may improve taste in transplastomic plants (Roh et al 2006). Below, we only discuss data about transplastomic plants with increased food functionality (Table 4).…”

Section: Biofortification-metabolic Engineering In Order To Enhance Nmentioning

confidence: 99%

Transplastomic plants for innovations in agriculture. A review

Wani

Sah

Sági

et al. 2015

Agron. Sustain. Dev.

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Food production has to be significantly increased in order to feed the fast growing global population estimated to be 9.1 billion by 2050. The Green Revolution and the development of advanced plant breeding tools have led to a significant increase in agricultural production since the 1960s. However, hundreds of millions of humans are still undernourished, while the area of total arable land is close to its maximum utilization and may even decrease due to climate change, urbanization, and pollution. All these issues necessitate a second Green Revolution, in which biotechnological engineering of economically and nutritionally important traits should be critically and carefully considered. Since the early 1990s, possible applications of plastid transformation in higher plants have been constantly developed. These represent viable alternatives to existing nuclear transgenic technologies, especially due to the better transgene containment of transplastomic plants. Here, we present an overview of plastid engineering techniques and their applications to improve crop quality and productivity under adverse growth conditions. These applications include (1) transplastomic plants producing insecticidal, antibacterial, and antifungal compounds. These plants are therefore resistant to pests and require less pesticides. (2) Transplastomic plants resistant to cold, drought, salt, chemical, and oxidative stress. Some pollution tolerant plants could even be used for phytoremediation. (3) Transplastomic plants having higher productivity as a result of improved photosynthesis. (4) Transplastomic plants with enhanced mineral, micronutrient, and macronutrient contents. We also evaluate field trials, biosafety issues, and public concerns on transplastomic plants. Nevertheless, the transplastomic technology is still unavailable for most staple crops, including cereals. Transplastomic plants have not been commercialized so far, but if this crop limitation were overcome, they could contribute to sustainable development in agriculture.

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Section: Biofortification-metabolic Engineering In Order To Enhance Nmentioning

confidence: 99%

Transplastomic plants for innovations in agriculture. A review

Wani

Sah

Sági

et al. 2015

Agron. Sustain. Dev.

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“…To characterize the expression of RDCP1 in our transgenic tobacco plants, we extracted total RNA with rri Reagent (MRC, USA; Roh et al, 2006) from the leaves of 28 transgenic lines of tobacco grown on MS media for 3 to 4 weeks. For northern blot analysis, total RNA was electrophoresed on a 1.2% denaturing formaldehyde/MOPS agarose gel.…”

Section: Rna Preparation and Northern Blot Analysismentioning

confidence: 99%

Transgenic tobacco expressing a ring domain-containing protein ofCapsicum annuum confers improved cold tolerance

et al. 2007

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Zinc finger proteins function in plant tolerances to stresses from cold, dehydration, and salt. To determine the mechanisms for those underlying defenses, we previously used cDNA microarrays and northern blot analysis to identify a gene for the ring zinc finger protein (RDCPt) from hot pepper (Capsicum annuum). In that study, we showed that the RDCP1 gene was strongly induced by cold stress and, to a lesser degree, by ABA and high salt. Here, we have used a Ti-plasmid and Agrobacteriummediated transformation to engineer RDCP1 under the control of the CaMV35S promoter for constitutive expression in tobacco. The resultant RDCP1 transgenic plants exhibit significantly increased tolerance to low temperatures. Moreover, some of those transgenics have greater drought tolerance. In addition, none of the RDCP1 transgenic plants show any visible alterations from the wild phenotype. These current results demonstrate the biological role of RDCP1 in conferring stress tolerance.

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“…Synthesis of non-native proteins may be enhanced by an increase in transgene copies or the insertion of regulated sequences (Roh et al, 2006). The aptamer regulates gene expression at the level of transcription or translation through DNA/RNA aptamer-small molecule interactions (Gossen and Bujard, 1992;Joel et al, 1997;Grate and Wilson, 2001).…”

mentioning

confidence: 99%

Aptamers improve the expression of a human granulocyte-macrophage colony stimulating factor in transgenicArabidopsis thaliana seeds

Wang

2007

J. Plant Biol.

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Domain IV nucleotides of human 7SL RNA comprise an approximately 50-nucleotide region with a highly conserved secondary structure consisting of two internal loops. We inserted these into a modified 3'-untranslated region of the human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene. The expression vector, under the control of a tissue-specific promoter derived from the soybean ~' subunit of ~3-conglycinin, was introduced into Arabidopsis thaliana by Agrobacteriurn.

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Accumulation of sweet protein monellin is regulated by thepsbA 5′UTR in tobacco chloroplasts

Cited by 20 publications

References 63 publications

Transplastomic plants for innovations in agriculture. A review

Transplastomic plants for innovations in agriculture. A review

Transgenic tobacco expressing a ring domain-containing protein ofCapsicum annuum confers improved cold tolerance

Aptamers improve the expression of a human granulocyte-macrophage colony stimulating factor in transgenicArabidopsis thaliana seeds

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