Accumulation of α-tocopherol and β-carotene in Euglena gracilis Cells Under Autotrophic and Mixotrophic Culture Conditions

Mokrosnop, V. M.; Polishchuk, Oleksandr; Zolotareva, Е. K.

doi:10.1134/s0003683816020101

Cited by 24 publications

(9 citation statements)

References 24 publications

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“…Attempts regarding enhancing microalgal vitamins production were already carried out. Optimization of α-tocopherol production has been done with Euglena gracilis Z, also maximizing β-carotene yield with mixotrophic cultivation [ 204 ]. UV-B light administration (until 4.4 kJ m −2 ) improved the production of α-tocopherol and β-carotene in Chlorella vulgaris [ 109 ].…”

Section: Microalgal Challenges For Vitamin Productionmentioning

confidence: 99%

Challenging microalgal vitamins for human health

et al. 2020

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Background Vitamins’ deficiency in humans is an important threat worldwide and requires solutions. In the concept of natural biofactory for bioactive compounds production, microalgae represent one of the most promising targets filling many biotechnological applications, and allowing the development of an eco-sustainable production of natural bioactive metabolites. Vitamins are probably one of the cutting edges of microalgal diversity compounds. Main text Microalgae can usefully provide many of the required vitamins in humans, more than terrestrial plants, for instance. Indeed, vitamins D and K, little present in many plants or fruits, are instead available from microalgae. The same occurs for some vitamins B (B12, B9, B6), while the other vitamins (A, C, D, E) are also provided by microalgae. This large panel of vitamins diversity in microalgal cells represents an exploitable platform in order to use them as natural vitamins’ producers for human consumption. This study aims to provide an integrative overview on vitamins content in the microalgal realm, and discuss on the great potential of microalgae as sources of different forms of vitamins to be included as functional ingredients in food or nutraceuticals for the human health. We report on the biological roles of vitamins in microalgae, the current knowledge on their modulation by environmental or biological forcing and on the biological activity of the different vitamins in human metabolism and health protection. Conclusion Finally, we critically discuss the challenges for promoting microalgae as a relevant source of vitamins, further enhancing the interests of microalgal “biofactory” for biotechnological applications, such as in nutraceuticals or cosmeceuticals.

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Section: Microalgal Challenges For Vitamin Productionmentioning

confidence: 99%

Challenging microalgal vitamins for human health

et al. 2020

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“…Ethanol had positive effect on growth of mixotrophically cultivated Euglena gracilis , with a 3-fold higher cell number, than in photoautotrophic control. Ethanol also strongly influenced metabolite accumulation in Euglena cells, with a 2-fold enhancement in β-carotene and total chlorophyll content, a 2-fold decrease in chlorophyll a / b ratio and a 7-fold decrease in α-tocopherol, compared to control [ 53 ]. Ethanol in presence of light was reported to considerably improve growth and α-tocopherol accumulation in cells of two Euglena gracilis strains (a wild strain and a chloroplast-deficient one), with growth improvement and α-tocopherol accumulation higher for the chloroplast-deficient strain, but total biomass and metabolite production higher for the wild strain [ 52 ].…”

Section: Effect Of Organic Solvents On Microalgae Growthmentioning

confidence: 99%

“…Industrial wastewaters and other effluents, containing these solvents within stimulatory ranges, could potentially improve biomass productivity of suitable microalgal strains. Methanol improved the productivity of some proteins [ 42 ], lipids [ 41 ] and pigments [ 43 ] and ethanol contributed to increase the amount of tocopherol [ 52 ], of some pigments [ 53 ], and lipid content [ 58 , 60 ], showing that these solvents can contribute not only to biomass increase, but also to increased production of target compounds. Ethanol was also reported to affect nucleic acids in microalgae cells.…”

Section: Strategies For Organic Solvent Use During Microalgal Cultmentioning

confidence: 99%

Effect of Organic Solvents on Microalgae Growth, Metabolism and Industrial Bioproduct Extraction: A Review

Miazek

Krátký

Šulc

et al. 2017

IJMS

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In this review, the effect of organic solvents on microalgae cultures from molecular to industrial scale is presented. Traditional organic solvents and solvents of new generation-ionic liquids (ILs), are considered. Alterations in microalgal cell metabolism and synthesis of target products (pigments, proteins, lipids), as a result of exposure to organic solvents, are summarized. Applications of organic solvents as a carbon source for microalgal growth and production of target molecules are discussed. Possible implementation of various industrial effluents containing organic solvents into microalgal cultivation media, is evaluated. The effect of organic solvents on extraction of target compounds from microalgae is also considered. Techniques for lipid and carotenoid extraction from viable microalgal biomass (milking methods) and dead microalgal biomass (classical methods) are depicted. Moreover, the economic survey of lipid and carotenoid extraction from microalgae biomass, by means of different techniques and solvents, is conducted.

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“…Wax esters, mainly composed of C14:0 saturated fatty acid, myristic acid, and myristyl alcohol 6 , synthesized under anaerobic conditions, holds promise for biodiesel and jet fuel production as an alternative to fossil fuels. Moreover, E. gracilis has been reported to be capable of producing dietary supplements α-tocopherol and β-carotene 7 , as well as assay vitamin B12 in body fluids 8 , waste water quality 9 , and ecotoxicity of engineered nanoparticles 10 . Thus, microalgae such as E. gracilis may be able to impact a large number of fields, including renewable bioenergy, nutritional and functional food, environment, and health-care.…”

Section: Introductionmentioning

confidence: 99%

Shape-based separation of microalga Euglena gracilis using inertial microfluidics

Muñoz

Goda

et al. 2017

Sci Rep

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Euglena gracilis (E. gracilis) has been proposed as one of the most attractive microalgae species for biodiesel and biomass production, which exhibits a number of shapes, such as spherical, spindle-shaped, and elongated. Shape is an important biomarker for E. gracilis, serving as an indicator of biological clock status, photosynthetic and respiratory capacity, cell-cycle phase, and environmental condition. The ability to prepare E. gracilis of uniform shape at high purities has significant implications for various applications in biological research and industrial processes. Here, we adopt a label-free, high-throughput, and continuous technique utilizing inertial microfluidics to separate E. gracilis by a key shape parameter-cell aspect ratio (AR). The microfluidic device consists of a straight rectangular microchannel, a gradually expanding region, and five outlets with fluidic resistors, allowing for inertial focusing and ordering, enhancement of the differences in cell lateral positions, and accurate separation, respectively. By making use of the shape-activated differences in lateral inertial focusing dynamic equilibrium positions, E. gracilis with different ARs ranging from 1 to 7 are directed to different outlets.

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Accumulation of α-tocopherol and β-carotene in Euglena gracilis Cells Under Autotrophic and Mixotrophic Culture Conditions

Cited by 24 publications

References 24 publications

Challenging microalgal vitamins for human health

Challenging microalgal vitamins for human health

Effect of Organic Solvents on Microalgae Growth, Metabolism and Industrial Bioproduct Extraction: A Review

Shape-based separation of microalga Euglena gracilis using inertial microfluidics

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