Accumulation of β-catenin by lithium chloride in porcine myoblast cultures accelerates cell differentiation

Yang, Yingjuan; Yang, Jinzeng; Liu, Rongxin; Li, Huixia; Luο, Xingguang; Yang, Gongshe

doi:10.1007/s11033-010-0328-3

Cited by 29 publications

(19 citation statements)

References 21 publications

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“…So far, most studies on the effect of LiCl on Wnt signaling were focused on established cell lines such as 3T3-L1 or C2C12 (60,67,79,82) or on the proliferation of satellite cells (61,63). There are only few reports about the effects of LiCl on differentiation of satellite cells (40,84). We showed here that treatment with 10 mM LiCl led to an extended differentiation of satellite cells (Fig.…”

Section: Expression Profiles Of Wnt4 During C2c12 and Satellite Cellsmentioning

confidence: 58%

Wnt4 activates the canonical β-catenin pathway and regulates negatively myostatin: functional implication in myogenesis

Bernardi

Gay

Fédon

et al. 2011

American Journal of Physiology-Cell Physiology

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Expression of Wnt proteins is known to be important for developmental processes such as embryonic pattern formation and determination of cell fate. Previous studies have shown that Wn4 was involved in the myogenic fate of somites, in the myogenic proliferation, and differentiation of skeletal muscle. However, the function of this factor in adult muscle homeostasis remains not well understood. Here, we focus on the roles of Wnt4 during C2C12 myoblasts and satellite cells differentiation. We analyzed its myogenic activity, its mechanism of action, and its interaction with the anti-myogenic factor myostatin during differentiation. Established expression profiles indicate clearly that both types of cells express a few Wnts, and among these, only Wnt4 was not or barely detected during proliferation and was strongly induced during differentiation. As attested by myogenic factors expression pattern analysis and fusion index determination, overexpression of Wnt4 protein caused a strong increase in satellite cells and C2C12 myoblast differentiation leading to hypertrophic myotubes. By contrast, exposure of satellite and C2C12 cells to small interfering RNA against Wnt4 strongly diminished this process, confirming the myogenic activity of Wnt4. Moreover, we reported that Wnt4, which is usually described as a noncanonical Wnt, activates the canonical ␤-catenin pathway during myogenic differentiation in both cell types and that this factor regulates negatively the expression of myostatin and the regulating pathways associated with myostatin. Interestingly, we found that recombinant myostatin was sufficient to antagonize the differentiation-promoting activities of Wnt4. Reciprocally, we also found that the genetic deletion of myostatin renders the satellite cells refractory to the hypertrophic effect of Wnt4. These results suggest that the Wnt4-induced decrease of myostatin plays a functional role during hypertrophy. We propose that Wnt4 protein may be a key factor that regulates the extent of differentiation in satellite and C2C12 cells. satellite cells; C2C12 myoblasts; muscle hypertrophy; muscle differentiation

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Section: Expression Profiles Of Wnt4 During C2c12 and Satellite Cellsmentioning

confidence: 58%

Wnt4 activates the canonical β-catenin pathway and regulates negatively myostatin: functional implication in myogenesis

Bernardi

Gay

Fédon

et al. 2011

American Journal of Physiology-Cell Physiology

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“…None of the cell lines showed increased apoptosis after Wnt3a stimulation but induction of myogenic differentiation markers (myogenin, myoD and myf5) indicating a possible role for Wnt signaling in promoting myodifferentiation in RMS, as suggested by in vitro studies. 11,31 Why myogenic differentiation markers induced by Wnt3a failed to arrest proliferation in embryonal RMS cell lines remains unexplained, although it is possible that the Pax-FKHR fusion gene protein, which imparts a growth advantage to alveolar RMS cells, may be directly inhibited by Wnt signaling. Other cytogenetic events in embryonal RMS may uncouple cell proliferation and cell differentiation.…”

Section: Effect Of Recombinant Wnt-3a On Proliferation and Apoptosis mentioning

confidence: 99%

“…The multifunctional nuclear transcription factor b-catenin is the major effector of the Wnt pathway and is involved in cell transformation. 6,7 Wnt signaling is also actively involved in myogenesis 5,[8][9][10][11] and embryonic somite patterning. 12,13 The Wnt pathway is crucial in cell proliferation, cell migration, polarity and cell death.…”

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confidence: 99%

Characterization of Wnt/β-catenin signaling in rhabdomyosarcoma

Rao

Cialfi

Dominici

et al. 2013

Laboratory Investigation

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Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children and accounts for about 5% of all malignant paediatric tumours. b-Catenin, a multifunctional nuclear transcription factor in the canonical Wnt signaling pathway, is active in myogenesis and embryonal somite patterning. Dysregulation of Wnt signaling facilitates tumour invasion and metastasis. This study characterizes Wnt/b-catenin signaling and functional activity in paediatric embryonal and alveolar RMS. Immunohistochemical assessment of paraffin-embedded tissues from 44 RMS showed b-catenin expression in 26 cases with cytoplasmic/membranous expression in 9/14 cases of alveolar RMS, and 15/30 cases of embryonal RMS, whereas nuclear expression was only seen in 2 cases of embryonal RMS. The potential functional significance of b-catenin expression was tested in four RMS cell lines, two derived from embryonal (RD and RD18) RMS and two from alveolar (Rh4 and Rh30) RMS. Western blot analysis demonstrated the expression of Wnt-associated proteins including b-catenin, glycogen synthase kinase-3b, disheveled, axin-1, naked, LRP-6 and cadherins in all cell lines. Cell fractionation and immunofluorescence studies of the cell lines (after stimulation by human recombinant Wnt3a) showed reduced phosphorylation of b-catenin, stabilization of the active cytosolic form and nuclear translocation of b-catenin. Reporter gene assay demonstrated a T-cell factor/lymphoid-enhancing factor-mediated transactivation in these cells. In response to human recombinant Wnt3a, the alveolar RMS cells showed a significant decrease in proliferation rate and induction of myogenic differentiation (myogenin, MyoD1 and myf5). These data indicate that the central regulatory components of canonical Wnt/b-catenin signaling are expressed and that this pathway is functionally active in a significant subset of RMS tumours and might represent a novel therapeutic target.

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“…GSK-3β activity is essential for β-catenin degradation and results in suppressing β-catenin/TCF activity (Yang et al, 2011). To examine whether GSK-3β is involved in the suppression of β-catenin/TCF activity by compounds 1 and 2, we treated HEK293 reporter cells with LiCl as a GSK-3β inhibitor.…”

Section: Effects Of Diarylheptonoids From Lesser Galangal On Wnt3a-inmentioning

confidence: 99%

Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway

Dong

Lee

Zhao

et al. 2015

Journal of Functional Foods

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Accumulation of β-catenin by lithium chloride in porcine myoblast cultures accelerates cell differentiation

Cited by 29 publications

References 21 publications

Wnt4 activates the canonical β-catenin pathway and regulates negatively myostatin: functional implication in myogenesis

Wnt4 activates the canonical β-catenin pathway and regulates negatively myostatin: functional implication in myogenesis

Characterization of Wnt/β-catenin signaling in rhabdomyosarcoma

Diarylheptanoids from lesser galangal suppress human colon cancer cell growth through modulating Wnt/β-catenin pathway

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