Accuracy of IgE antibody laboratory results

Wood, Robert A.; Segall, Nathan; Ahlstedt, S.; Williams, P. Brock

doi:10.1016/s1081-1206(10)60618-7

Cited by 114 publications

(82 citation statements)

References 24 publications

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“…Until now, the predictive values of sIgE in food allergy have been determined mostly using the ImmunoCAP system, making interpretation of the values measured by other assays difficult. Some reports argue that 3gAllergy overestimates IgE values 4, 6 However, assays from different vendors commonly produce disparate values, and in any assay comparison, it is expected that there will be differences between the reference assay and the comparison assay. In one study, for example, 3gAllergy appeared to underperform ImmunoCAP 4.…”

Section: Discussionmentioning

confidence: 99%

“…Some reports argue that 3gAllergy overestimates IgE values 4, 6 However, assays from different vendors commonly produce disparate values, and in any assay comparison, it is expected that there will be differences between the reference assay and the comparison assay. In one study, for example, 3gAllergy appeared to underperform ImmunoCAP 4. However, at least one study has shown that in terms of assay performance – including accuracy and reproducibility – the 3gAllergy system appears to be comparable to the ImmunoCAP system 1.…”

Section: Discussionmentioning

confidence: 99%

“…Binding is dependent on the assay's allergen extract source and physical properties of the immobilized allergen that affect epitope conformation. Thus, results expressed as units do not generate the same value using different manufacturer's methods, leading to the occurrence of considerable discrepancy 4, 5, 6. Because of this, it is important that physicians interpreting the results understand the predictive sIgE levels of the particular assay they use.…”

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confidence: 99%

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Predictive values of egg‐specific IgE by two commonly used assay systems for the diagnosis of egg allergy in young children: a prospective multicenter study

Furuya

Nagao

Sato

et al. 2016

Allergy

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BackgroundSpecific IgE (sIgE) is often used to predict oral food challenge (OFC) outcomes in food allergy, but interpretation of the results may vary depending on the assay method employed and the patient population tested. The aim of this study was to use two commercial assay systems to determine egg‐sIgE values predictive of allergy within the most common populations treated at pediatric clinics.MethodsIn a multicenter prospective study, 433 children with suspected or confirmed egg allergy underwent oral challenge (OFC) using cooked egg (CE) and raw egg (RE) powders to diagnose either true allergy in 1‐year‐old (group A, n = 220) or tolerance in 2‐ to 6‐year‐old (group B, n = 213). Egg white (EW)‐ and ovomucoid (OM)‐sIgE values were measured using the ImmunoCAP ® sIgE (ImmunoCAP) and the IMMULITE ® 2000 3 gAllergy™ (3gAllergy) systems. Children were recruited from six primary care clinics and 18 hospitals in Japan.ResultsReceiver‐operating characteristic (ROC) curve analysis yielded similar areas under the curve (AUC) for the two assays (0.7–0.8). The optimal cutoff values and the probability curves (PCs) of the sIgE by the two assays to predict CE and RE OFC outcomes were determined for both groups. Values for 3gAllergy were higher than for ImmunoCAP; however, correlation of sIgE and predicted probability calculated by PCs were strong between the two methods.ConclusionsCutoff values and PCs for egg‐sIgE established using both ImmunoCAP and 3gAllergy may be useful for predicting egg allergy in early childhood patient populations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Predictive values of egg‐specific IgE by two commonly used assay systems for the diagnosis of egg allergy in young children: a prospective multicenter study

Furuya

Nagao

Sato

et al. 2016

Allergy

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“…Fluorometric assays have permitted the development of automatized systems, resulting in improved reproducibility, diminished operator involvement, with reduction of mistakes, and increased sensitivity and specificity when compared with other innovative methods based on chemiluminescence [27,28]. FEIA technology opens the possibility to screen sIgE to several allergens at the same time and with few volume of sample [29].…”

Section: Fluorescent Enzyme Immune Assay (Feia)mentioning

confidence: 99%

Allergen-Based Diagnostic: Novel and Old Methodologies with New Approaches

Salazar¹,

Velázquez-Soto²,

Ayala-Balboa³

et al. 2017

Allergen

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This chapter is an extensive review of allergen-based diagnostic methodologies including old techniques such as skin prick test, radio-allergo sorbent test, enzymelinked immunosorbent assay, and fluorescent-enzyme immunosorbent assay. Novel technologies include functional tests by flow cytometry and molecular allergy based on multiplex immunoassays. We also review the importance of biochemical characteristics of allergens, sensitivity and specificity, cross-reaction between allergens, utility, reproducibility, interpretation, and methodologies for discovery of epitopes for diagnostic or therapeutic use.

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“…Согласно лаборатор-ным стандартам ВОЗ 1 МЕ/мл (МЕ -международ-ная единица) соответствует 2,4 нг/мл иммуноглобу-лина Е. Концентрацию IgE определяют либо в МЕ/ мл, либо в кЕ/л (кЕ/л -килоединица). Сравнитель-ные исследования воспроизводимости, чувстви-тельности и специфичности определения уровня общего IgE на автоматизированных тест-системах ImmunoCap (ThermoFisher, «Phadia»), Immulite («Siemens»), двух ведущих компаний, специализи-рующихся на диагностике аллергии, продемонстри-ровали полное совпадение результатов тестов и со-ответствие стандарту 75/502 [15][16][17][18].…”

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Diagnostic value of serum total IgE the primary marker of allergic diseases

Mokronosova¹

2015

Lab. sluzh.

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Ключевые слова: иммуноглобулин е, IgE-опосредованная патология, анти-IgE терапия.Discovery of immunoglobulin e in 1967-1968 belongs to Johansson sGO and ishizaka K. the concentration of ige in the serum is 0.05% of all immunoglobulins. molecule ige binds to Fcεr receptor on the surface of mast cells and basophils. the triggering of the mast cells's activation due to contact of the ige molecule with the receptor is the basic mechanism of most common allergic reactions characterized by an immediate type hyperreactivity. serum total ige -is an important diagnostic marker which is used to screen for atopic diseases , the diagnosis of helminthiasis and various immune diseases. the level of total immunoglobulins is used as the primary marker when assigning and monitoring the anti-ige therapy.

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Accuracy of IgE antibody laboratory results

Cited by 114 publications

References 24 publications

Predictive values of egg‐specific IgE by two commonly used assay systems for the diagnosis of egg allergy in young children: a prospective multicenter study

Predictive values of egg‐specific IgE by two commonly used assay systems for the diagnosis of egg allergy in young children: a prospective multicenter study

Allergen-Based Diagnostic: Novel and Old Methodologies with New Approaches

Diagnostic value of serum total IgE the primary marker of allergic diseases

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