Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia

Qu, Jiuxin; Gu, Li; Wu, Jiang; Dong, Jie; Pu, Zenghui; Gao, Yan; Hu, Ming; Zhang, Yongxiang; Gao, Feng; Cao, Bin; Wang, Chen

doi:10.1186/1471-2334-13-172

Cited by 53 publications

(59 citation statements)

References 26 publications

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“…First, we used a passive agglutination test measuring the mixed antibody titer of M. pneumoniae IgG, IgA and IgM. Ideally, serology, culture and fluorescence quantitative polymerase chain reaction (FQ-PCR) should be incorporated in the establishment of M. pneumonia infection in clinical practice [27]. However, culture is slow and FQ-PCR is expensive, and both have low sensitivity [27].…”

Section: Discussionmentioning

confidence: 99%

Mycoplasma pneumoniae infection in hospitalized adult patients with community-acquired pneumonia in China

Shangguan

Sun

Zhang

et al. 2014

J Infect Dev Ctries

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Introduction: This study aimed to investigate the prevalence, clinical and radiographic features, and antibiotic responses of Mycoplasma pneumoniae (M. pneumoniae) infections in hospitalized adults with community-acquired pneumonia (CAP) in China. Methodology: Serum specimens collected from 189 CAP patients in both acute phase and convalescence were tested for IgG, IgA, and IgM mixed antibodies specific to M. pneumoniae. The clinical and radiographic characteristics and efficacy of three antibiotic regimens were compared between patients with M. pneumoniae infection and those without. Results: Among 189 CAP patients, 88 (46.6%) were positive for M. pneumoniae infection. Compared to the negative patients, patients with M. pneumoniae infection were significantly younger, had higher rates of dry cough, and had white blood cell counts of <10 10 /L, but had less purulent sputum. Radiography further showed more centrilobular nodules, ground-glass opacities, tree-in-bud patterns and thickened bronchovascular bundles, but less pleural effusion and larger tracts of real opacities in patients with M. pneumoniae infections. Among the three regimens used, patients with moxifloxacin required significantly shorter fever abatement, treatment, and hospitalization times than those with azithromycin plus ceftriaxone and ceftriaxone only. Conclusions: M. pneumoniae infection was present in almost half of the CAP population in east China, with some distinct clinical and radiographic features. Moxifloxacin was an effective antibiotic for this infection.

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Section: Discussionmentioning

confidence: 99%

Mycoplasma pneumoniae infection in hospitalized adult patients with community-acquired pneumonia in China

Shangguan

Sun

Zhang

et al. 2014

J Infect Dev Ctries

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“…Because of the absence of cell walls with M. pneumoniae, macrolide antibiotics are recognized generally as the first-choice agents in clinical treatment (3,8,9). However, with the widespread use of the drug, increasing numbers of macrolide-resistant M. pneumoniae have been reported in the past decade, especially in Asia, Europe, and the United States (6,(10)(11)(12).…”

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confidence: 99%

Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China

Zhou

Chen

et al. 2015

Antimicrob Agents Chemother

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dMycoplasma pneumoniae is a major pathogen causing community-acquired pneumoniae (CAP), which is generally treated with macrolides. In recent years, however, although macrolide-resistant M. pneumoniae has been reported frequently, particularly in China, very little is known about the prevalence of macrolide-resistant M. pneumoniae infection in adults. In this study, we survey the macrolide-resistant M. pneumoniae in adults in Zhejiang province and characterize the mechanisms of resistance to macrolide. Six hundred fifty throat swab samples were collected from adult patients with CAP from January 2012 to August 2014. These samples were assayed by nested PCR and then cultivated for M. pneumoniae. All isolates were sequenced to determine the mutation in domain V of the 23S rRNA gene. The activities of 10 antibiotics against macrolide-resistant M. pneumoniae isolates were also investigated in vitro. Moreover, restriction fragment length polymorphism (RFLP) analysis of the amplified P1 gene was used to type 50 resistant strains. One hundred percent (71/71) of M. pneumoniae strains isolated from adults with CAP were resistant to erythromycin (MIC ‫؍‬ 128 to >256 g/ml), clarithromycin (MIC ‫؍‬ 128 to >256 g/ml), and azithromycin (MIC ‫؍‬ 32 to >64 g/ml). Furthermore, all macrolide-resistant M. pneumoniae strains identified had an A2063G mutation in domain V of the 23S rRNA gene. Forty-six resistant strains (92.0%) were classified into type I strain on the basis of P1 gene PCR-RFLP analysis. According to these findings, it is suggested that macrolide-resistant M. pneumoniae infection is very prevalence among adults in Zhejiang province. Thus, there is necessary to perform the epidemiological monitoring of macrolide-resistant M. pneumoniae in the future.

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“…In accordance with the current study data, Morozumi (26) and Medjo (22) found similar specificities, while the sensitivity rates were higher in their studies than those of the current study. On the other hand, some researchers reported lower sensitivity and specificity of PCR method in comparison with those of the IgG serology (24,27). These differences in the results of such studies could be due to PCR types (conventional PCR or RT-PCR), gene targets used for amplification reaction, sample type, or time of sampling.…”

Section: Discussionmentioning

confidence: 79%

Detection of Mycoplasma pneumoniae Among Children with Pneumonia Using Bacterial Culture, Polymerase Chain Reaction, and the Enzyme-linked Immunosorbent Assay Techniques in Ahvaz, Iran

Amin

Abadi

Navidifar

et al. 2018

Jundishapur J Microbiol

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Background: Mycoplasma pneumoniae is one of the most common causes of atypical pneumonia, which is almost asymptomatic and self-limited. Objectives: The current study aimed at investigating the prevalence of M. pneumoniae among children with pneumonia in Ahvaz, Iran, using bacterial culture growth, polymerase chain reaction (PCR), and serology tests. Methods: A total of 136 throat swab and serum specimens were collected from patients with pneumonia. The specimens were cultured on pleuropneumonia-like organisms (PPLO) agar. Molecular identification of the throat swab specimens was performed using the amplification of P1 gene. Determination of M. pneumoniae-specific antibodies (IgG and IgM) in the sera was carried out by the enzyme-linked immunosorbent assay (ELISA) technique. Results: In the current study, the acute infection was detected in 16 cases. Moreover, 3 out of 136 cases had positive results in their bacterial culture. Mycoplasma pneumoniae DNA was detected in 11 of the 136 cases. An acceptable titer of IgM was observed in 12 cases. On the other hand, 4-fold or greater titer of IgG was detected in 14 cases. Conclusions:The results of the current study suggested that the combination of PCR and the serology results were effective to detect M. pneumoniae. Moreover, the combination of PCR and IgM results can detect all cases of acute infection with M. pneumoniae in children.

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Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia

Cited by 53 publications

References 26 publications

Mycoplasma pneumoniae infection in hospitalized adult patients with community-acquired pneumonia in China

Mycoplasma pneumoniae infection in hospitalized adult patients with community-acquired pneumonia in China

Macrolide-Resistant Mycoplasma pneumoniae in Adults in Zhejiang, China

Detection of Mycoplasma pneumoniae Among Children with Pneumonia Using Bacterial Culture, Polymerase Chain Reaction, and the Enzyme-linked Immunosorbent Assay Techniques in Ahvaz, Iran

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