Accuracy of Intraocular Pressure Measurements in New Zealand White Rabbits

Lim, Kin Sheng; Wickremasinghe, Sanjeewa; Cordeiro, M. Francesca; Bunce, Catey; Khaw, PT

doi:10.1167/iovs.04-0610

Cited by 53 publications

(53 citation statements)

References 13 publications

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“…With regard to the accuracy, there was a strong correlation between the IOP values obtained with ocular manometry and the IOP values obtained with the Tono-Pen XL ® and Perkins ® tonometers ( Figure 2); however, the Perkins ® tonometer was more accurate than the Tono-Pen XL ® tonometer, producing measurements that were much closer to the real IOP values measured with manometry (Table 1). The Tono-Pen ® XL tonometer underestimated IOPs between 15 and 50mmHg in horses and cattle, which was in agreement with other studies (PRIES et al, 1990;ANDRADA MÁRQUES et al, 2003;PASSAGLIA et al, 2004;LIM et al, 2005;KALESNYKAS & UUSITALO, 2007). Some studies comparing the Tono-Pen XL ® tonometer with other tonometers, such as the Perkins ® and TonoVet ® tonometers in rabbits (KALESNYKAS & UUSITALO, 2007) and the Goldmann tonometer in humans (FRENKEL et al, 1988;IESTER et al, 2001) …”

Section: Discussionsupporting

confidence: 91%

Intraocular pressure measurements with the Tono-Pen XL® and Perkins® applanation tonometers in horses and cattle

et al. 2013

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Section: Discussionsupporting

confidence: 91%

Intraocular pressure measurements with the Tono-Pen XL® and Perkins® applanation tonometers in horses and cattle

et al. 2013

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“…A possible animal model for these experiments appears to be the New Zealand white albino rabbit (NZWAR). This is an established model for the investigation of corneal disease (Nakamura et al 2003, Tungsiripat et al 2003, Ivarsen et al 2004, Mimura et al 2005 and AH dynamics (Tsukahara et al 1986, Green et al 1989, Munden & Schmidt 1992, Lim et al 2005. The corticosteroid hormone profile is similar to humans (i.e.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the prereceptor regulation of glucocorticoids in the anterior segment of the rabbit eye

Onyimba¹,

Vijapurapu²,

Curnow³

et al. 2006

Journal of Endocrinology

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The prereceptor regulation of glucocorticoids (GCs) by 11b-hydroxysteroid dehydrogenase type-1 (11b-HSD1), a bidirectional isozyme that interconverts active (cortisol) and inactive (cortisone) GCs, is an established determinant of GC function in tissues such as liver, adipose and bone. Although the therapeutic use of GCs is abundant in ophthalmic practice, where GC interactions with nuclear receptors modulate gene transcription, the prereceptor regulation of endogenous cortisol is not well described in ocular tissues. Recent descriptive studies have localised 11b-HSD1 to the human corneal epithelium and non-pigmented epithelium (NPE) of the ciliary body, indicating a link to corneal epithelial physiology and aqueous humour production. In this study, we characterise the functional aspects of the autocrine regulation of GCs in the anterior segment of the rabbit eye. Using our in-house generated primary antibody to human 11b-HSD1, immunohistochemical analyses were performed on paraffin-embedded sections of whole New Zealand white albino rabbits, (NZWAR) eyes. As in human studies, 11b-HSD1 was localised to the corneal epithelium and the NPE. No staining was seen in the albino 'pigmented' ciliary epithelium. Specific enzyme assays for oxo-reductase (cortisone/cortisol) and dehydrogenase (cortisol/cortisone) activity indicated predominant 11b-HSD1 oxo-reductase activity from both the intact ciliary body tissue (nZ12, median 2$1 pmol/mg per h and range 1$25-2$8 pmol/mg per h; PZ0$006) and primary cultures of corneal epithelial cells (nZ12, median 3$0 pmol/mg per h and range 1$0-7$4 pmol/mg per h, PZ0$008) compared with dehydrogenase activity (median 1$0 pmol/mg per h and range 0$5-2$0 pmol/mg per h; median 0$5 pmol/mg per h and range 0$25-1$9 pmol/mg per h respectively). These findings were supported by expression of 11b-HSD1 protein as visualised by Western blotting of ciliary body tissue and immunocytochemistry of corneal epithelial cells. Reduction of corneal epithelial cell proliferation was seen after primary cultures were co-incubated with cortisol and cortisone. 11b-HSD1 activity was not demonstrated in naïve conjunctival fibroblasts or corneal stromal keratocytes. Our results indicate that the distribution of 11b-HSD1 in the rabbit resembles that of the human eye and activates cortisone to cortisol in both corneal and uveal tissues. The NZWAR provides a suitable in vivo model for the further evaluation of 11b-HSD1 activity in the eye, especially its role in corneal epithelial and ciliary body physiology.

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“…All tonometric measurements were performed by the same ophthalmic surgeon. To correct tonometry estimates that provide a closer approximation of the actual IOP in rabbits; Equation 1 was used as derived by Lim et al 19 from a linear regression study in which they demonstrated that the Tonopen TM XL showed the least error in estimation of true pressure. However, there was a high degree of variability in the measurements, especially at higher IOPs, where it significantly undervalued the IOP.…”

Section: Postoperative Monitoring Of the Rabbitsmentioning

confidence: 99%

In Vivo Evaluation of a Biodegradable Donut-Shaped Minitablet for Prolonged Posterior Segment Drug Delivery in the Rabbit Eye Model

Choonara

Pillay

Carmichael

et al. 2011

Journal of Pharmaceutical Sciences

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Accuracy of Intraocular Pressure Measurements in New Zealand White Rabbits

Abstract: All tonometers underestimate IOP, with increasing inaccuracy at higher pressures. The Perkins applanation tonometer was the most reliable for measuring IOP in the range of 0 to 50 mm Hg in New Zealand White rabbits.

Cited by 53 publications

References 13 publications

Intraocular pressure measurements with the Tono-Pen XL® and Perkins® applanation tonometers in horses and cattle

Intraocular pressure measurements with the Tono-Pen XL® and Perkins® applanation tonometers in horses and cattle

Characterisation of the prereceptor regulation of glucocorticoids in the anterior segment of the rabbit eye

In Vivo Evaluation of a Biodegradable Donut-Shaped Minitablet for Prolonged Posterior Segment Drug Delivery in the Rabbit Eye Model

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