Accuracy of the haemoglobin alkaline denaturation test for detecting maternal blood contamination of fetal blood samples for prenatal karyotyping

Sepúlveda, Waldo; Be, Cecilia; Youlton, R; Gutiérrez, Jorge; Carstens, Eduardo

doi:10.1002/(sici)1097-0223(199910)19:10<927::aid-pd668>3.3.co;2-8

Cited by 5 publications

(5 citation statements)

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“…The procedures were performed under sterile conditions with continuous ultrasound guidance after informed consent was obtained. In pregnancies undergoing fetal blood sampling, the purity of blood was confirmed as previously reported17. Fetal samples were processed in one of three accredited laboratories, where karyotype results were obtained using standard cytogenetic techniques.…”

Section: Methodsmentioning

confidence: 93%

Chromosomal abnormalities in fetuses with open neural tube defects: prenatal identification with ultrasound

Sepúlveda

Corral

Ayala

et al. 2004

Ultrasound in Obstet & Gyne

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Section: Methodsmentioning

confidence: 93%

Chromosomal abnormalities in fetuses with open neural tube defects: prenatal identification with ultrasound

Sepúlveda

Corral

Ayala

et al. 2004

Ultrasound in Obstet & Gyne

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“…Maternal and cord samples were collected in differently sized tubes to avoid the chance of mislabelling. The haemoglobin alkaline denaturation test was used in order to exclude the admixture of maternal blood contamination with the fetal blood samples (Sepulveda et al 1999). Sera were stored at −70 °C until further use.…”

Section: Methodsmentioning

confidence: 99%

Maternal filarial infection: association of anti-sheath antibody responses with plasma levels of IFN-γand IL-10

et al. 2013

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Maternal filarial infection influences the risk of acquiring infection and development of immunity in children. Here we have analysed the blood samples of 60 mothers (24 infected and 36 uninfected) and their corresponding cord bloods to assess the impact of maternal infection on the anti-sheath antibodies and cytokine production in neonates born from them. About 69·4% of non-infected mothers and their cord bloods showed the presence of anti-sheath antibodies, while only 16·6% of the cord bloods from infected mothers were positive for it. The IL-10 level was significantly high in cord bloods of infected mothers compared with non-infected mothers. At the same time the IL-10 level was also observed to be remarkably high in cord bloods of both infected and non-infected mothers negative for anti-sheath antibody. In contrast, IFN-γ levels were significantly high in cord bloods of non-infected mothers compared with infected mothers and the increment was prominent in cord bloods of both infected and non-infected mothers positive for anti-sheath antibody. The study reveals that the presence or absence of anti-sheath antibodies in association with cytokines skews the filarial specific immunity to either Th1 or Th2 responses in neonates. This may affect the natural history of filarial infection in early childhood.

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“…Paired maternal and cord blood samples ( n = 145; term births = 131, post-term births = 14 and no pre-term births) were collected from the O & G Department of Khurda Hospital. The admixture of maternal and cord blood was ruled out by using an alkali denaturation test, as described previously (Scpulveda et al , 1999). Each mother's age, parity status and level of formal education were recorded.…”

Section: Methodsmentioning

confidence: 99%

Increased IgG antibody responses to excretory/secretory antigens in neonates born from mothers infected with filarial nematodes

et al. 2016

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The present investigation aimed to evaluate the extent to which maternal filarial infection influences IgG subclass immune responses in the cord blood of neonates. Prevalence of antigenaemia was detected using an Og4C3 assay. Filaria-specific IgG subclasses against excretory/secretory antigens were measured by ELISA. Transplacental transfer of circulating filarial antigen (CFA) was observed from 34.8% of CFA-positive mothers to their respective cord bloods. Filaria-specific IgG1, IgG2 and IgG4 responses were significantly higher among cord bloods of infected mothers compared to cord bloods of uninfected mothers. In contrast, the IgG3 response was significantly higher among cord bloods of uninfected mothers. The study shows that transplacental transfer of filarial antigens and filaria-specific IgG4 occurs more in mothers having high worm burdens, and transfer of filaria-specific IgG3 occurs more in the cord blood of uninfected mothers. The findings of the study provide evidence for the development of prenatal sensitization to filarial antigens in utero, and high filaria-specific IgG4 in cord blood may serve as a marker for in-utero sensitization.

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Accuracy of the haemoglobin alkaline denaturation test for detecting maternal blood contamination of fetal blood samples for prenatal karyotyping

Cited by 5 publications

References 0 publications

Chromosomal abnormalities in fetuses with open neural tube defects: prenatal identification with ultrasound

Chromosomal abnormalities in fetuses with open neural tube defects: prenatal identification with ultrasound

Maternal filarial infection: association of anti-sheath antibody responses with plasma levels of IFN-γand IL-10

Increased IgG antibody responses to excretory/secretory antigens in neonates born from mothers infected with filarial nematodes

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