Accurate diagnosis of endometriosis using serum microRNAs

Moustafa, Sarah; Burn, Martina S.; Mamillapalli, Ramanaiah; Nematian, Sepide; Flores, Valerie A.; Taylor, Hugh S.

doi:10.1016/j.ajog.2020.02.050

Cited by 119 publications

(108 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are some cases of inconsistency between these studies. For instance, expression of miR-451a has been shown to be up-regulated in serum ( 13 ), exosomes ( 20 ) and endometriosis lesions of patients with endometriosis ( 23 ) as well as samples obtained from mouse models of endometriosis ( 22 ). However, another study has reported downregulation of miR-451 in the eutopic endometrial tissues of patients with endometriosis compared with control tissues ( 53 ).…”

Section: Discussionmentioning

confidence: 99%

“…Importantly, neither phase of menstrual cycle nor use of hormonal medicines affected the expression levels in these miRNAs. Thus, authors concluded the potential of the miRNAs panel in detection of endometriosis in clinical setting (13). Table 3 summarizes the results of studies which reported diagnostic value of miRNAs in endometriosis.…”

Section: Mirnas and Endometriosismentioning

confidence: 99%

See 1 more Smart Citation

Role of Non-coding RNAs in the Pathogenesis of Endometriosis

2020

View full text Add to dashboard Cite

Endometriosis is a disorder characterized by the presence of endometrial glands and stroma like lesions outside of the uterus. Although several hypothesis have tried to explain the underlying cause of endometriosis, yet the main cause remained obscure. Recent studies have shown contribution of non-coding RNAs in the pathogenesis of endometriosis. Two classes of these transcripts namely long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have mostly attracted attention of researchers. Several studies have reported aberrant expression of these transcripts in affected tissues from patients as well as animal models. Modulation of important signaling pathways such as PI3K/AKT, P38-MAPK, ERK1/2-MAPK and Wnt-β catenin by miRNAs and lncRNAs have potentiated these molecules as biomarkers or therapeutic agents in endometriosis. Single nucleotide polymorphisms with miR-126, miR-143 and miR-146b have been associated with risk of endometriosis. Moreover, miRNAs and lncRNAs control inflammatory responses, cell proliferation, angiogenesis and tissue remodeling, thus understanding the role of these transcripts in endometriosis is a possible way to develop novel diagnostic tests and therapeutic targets for this disorder.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Mirnas and Endometriosismentioning

confidence: 99%

Role of Non-coding RNAs in the Pathogenesis of Endometriosis

2020

View full text Add to dashboard Cite

show abstract

“…RT-qPCR: reverse transcription quantitative polymerase chain reaction; miR: microRNA; ANXA2: Annexin A2; NC: negative control. 6 Analytical Cellular Pathology…”

Section: Mir-342 Negatively Regulates Anxa2 Expressionmentioning

confidence: 99%

MicroRNA-342 Promotes the Malignant-Like Phenotype of Endometrial Stromal Cells via Regulation of Annexin A2

Sun

Wang

et al. 2021

Analytical Cellular Pathology

View full text Add to dashboard Cite

The relevance of miRNA- (miR-) 342 to endometriosis has been highlighted, while its function in regulating the malignant-like phenotype of endometrial stromal cells which demonstrate epigenetic abnormalities that alter expression of transcription factors, remains unclear. Therefore, we sought to characterize the effects of miR-342 in endometrial stromal cell proliferation by regulating Annexin A2 (ANXA2). We first characterized the levels of miR-342 and ANXA2 in 31 cases of normal endometrium from patients with grade II-III cervical intraepithelial neoplasia or patients with hysterectomy versus ectopic endometrial tissues of 42 patients with endometriosis. miR-342 was upregulated, while ANXA2 was downregulated in ectopic endometrial tissues. Bioinformatics website and dual-luciferase reporter assay revealed that miR-342 negatively modulated ANXA2 expression. Following loss- and gain-of-function approaches, CCK-8, Transwell, and flow cytometry demonstrated that overexpression of miR-342 markedly increased cell proliferation, migration, and invasion but inhibited cell apoptotic ratio of endometrial stromal cells, which was reversed by ANXA2 elevation. Further, overexpressed miR-342 activated the PI3K/AKT/mTOR signaling pathway, as evidenced by upregulated levels of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR. Taken together, miR-342 targets ANXA2 to activate the PI3K/AKT/mTOR signaling pathway, thereby promoting the malignant-like phenotype of endometrial stromal cells, highlighting miR-342 inhibition as a promising approach for the treatment of endometriosis.

show abstract

“…Excitingly, in a recent prospective study by Mustafa et al, 100 patients undergoing gynecologic surgery for pelvic pain and/or infertility showed that a set of six miRNAs were able to accurately distinguish between endometriosis and other gynecologic conditions, regardless of current hormonal treatments or menstrual cycle phase [12]. This study was the first to apply expression testing of a panel of miRNAs previously determined as unique to endometriosis to predict the diagnosis of endometriosis in a patient with pelvic pain and/or infertility.…”

Section: Editorialmentioning

confidence: 99%