Accurate evaluation and verification of varietal ranking for flooding tolerance at the seedling stage in barley (<i>Hordeum vulgare</i> L.)

Abstract

Soil flooding or waterlogging is a major abiotic stress in upland crops. In barley, there have been several reported studies of selection for flooding-tolerant genotypes, but it is difficult to obtain varietal rankings that are consistent among researchers. Our objectives were to establish experimental conditions that could be applied by other research groups and to verify the varietal ranking conducted in an earlier study. We conducted greenhouse experiments on 14 barley varieties. At the 2.5-leaf stage, they were flooded with 0% or 0.1% soluble starch solution (mimicking reducing conditions). At 13 to 15 days after the start of treatment, the degree of leaf injury and the shoot dry weight ratio (treatment:control) were recorded. Reliable and highly repeatable results were obtained for the criterion of leaf injury under reducing conditions, whereas shoot dry weight ratio was unstable. The varieties OUJ820 and OUA301 were highly tolerant, whereas OUA002 and OUJ247 were sensitive; these results matched those of the earlier study. The experimental conditions that we developed here may be useful for selection testing and genetic analysis of flooding tolerance in other laboratories.

QTLs for constitutive aerenchyma from Zea nicaraguensis improve tolerance of maize to root-zone oxygen deficiency

“…Quantitative trait loci (QTLs) associated with CAF in Z. nicaraguensis have been examined using populations of maize inbred line Mi29 crossed with Z. nicaraguensis (Mano and Omori, 2008, 2009), maize inbred line B64 crossed with Z. nicaraguensis (Mano et al , 2007), and S 1 and S 2 segregants of Z. nicaraguensis (Mano et al , 2012). So far, seven QTLs have been identified (Mano et al , 2016).…”

Enhanced waterlogging tolerance in barley by manipulation of expression of the N‐end rule pathway E3 ligase PROTEOLYSIS6

“…It has been suggested that selecting genotypes with least reduction in photosynthetic rate or total chlorophyll content may facilitate identification of tolerant types (Pang et al, 2004). Quantitative trait loci (QTL) associated with tolerance to waterlogging in barley have been identified using combinations of leaf chlorosis, plant biomass production and plant death (Li et al, 2008(Li et al, , 2013Mano and Takeda, 2012;Zhou, 2011;Zhou et al, 2012).…”

The effect of waterlogging on yield and seed quality at the early flowering stage in Brassica napus L.

“…Plant growth is also inhibited by waterlogging, and dry matter accumulation is reduced (Osman et al, 2013;Zou et al, 2014a). Certainly, the yield of important crops including maize (Qiu et al, 2007), wheat (Setter et al, 2009;Yu and Chen, 2013), rapeseed (Zou et al, 2014a) and barley (Mano and Takeda, 2012) decrease drastically due to waterlogging.…”