In the past three years, the sensitivity and the performance of the Penning trap mass spectrometer ISOLTRAP have been enhanced significantly. These improvements, which range from technical developments to systematic studies of the various factors contributing to the uncertainty of the final mass result, now allow mass measurements of short-lived radionuclides with half-lives of less than 100 ms and with a precision of better than 10 −8 . Using a newly developed carbon cluster ion source, ISOLTRAP can perform absolute mass measurements relative to the microscopic mass standard 12 C. These developments are reviewed as pertaining to the extension of ISOLTRAP mass measurements to higher precision and shorter half-lives and to molecular mass measurements.