Accurate Serodiagnosis Of B19 Parvovirus Infections By Measurement Of Igg Avidity

Söderlund, Maria; Brown, Caroline; Cohen, B. J.; Hedman, Klaus

doi:10.1093/infdis/171.3.710

Cited by 87 publications

(65 citation statements)

References 12 publications

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“…The specimens comprised (i) 1,393 sera collected during 1983-1997 from 1,393 patients with rash, fever, or other constitutional symptoms, initially studied with negative results for rubella and measles (45), Sindbis virus (46), or hantavirus disease (47); and (ii) 247 sera collected during 1992-1993 from 247 patients with serologically confirmed erythema infectiosum (48,49). The sera were studied in pools of 10 (10 l each), taking 20 l per pool for DNA purification.…”

Section: Methodsmentioning

confidence: 99%

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Norja

Hokynar

Aaltonen³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

253

216

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Human erythrovirus is a minute, single-stranded DNA virus causing many diseases, including erythema infectiosum, arthropathy, and fetal death. After primary infection, the viral genomes persist in solid tissues. Besides the prototype, virus type 1, two major variants (virus types 2 and 3) have been identified recently, the clinical significance and epidemiology of which are mostly unknown. We examined 523 samples of skin, synovium, tonsil, or liver (birth year range, 1913-2000), and 1,640 sera, by qualitative and quantitative molecular assays for the DNA of human erythroviruses. Virus types 1 and 2 were found in 132 (25%) and 58 (11%) tissues, respectively. DNA of virus type 1 was found in all age groups, whereas that of type 2 was strictly confined to those subjects born before 1973 (P < 0.001). Correspondingly, the sera from the past two decades contained DNA of type 1 but not type 2 or 3. Our data suggest strongly that the newly identified human erythrovirus type 2 as well as the prototype 1 circulated in Northern and Central Europe in equal frequency, more than half a century ago, whereafter type 2 disappeared from circulation. Type 3 never attained wide occurrence in this area during the past >70 years. The erythrovirus DNA persistence in human tissues is lifelong and represents a source of information about our past, the Bioportfolio, which, at the individual level, provides a registry of one's infectious encounters, and at the population level, a database for epidemiological and phylogenetic analyses.epidemiology ͉ gene therapy ͉ parvovirus ͉ phylogeny ͉ single-stranded DNA

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Section: Methodsmentioning

confidence: 99%

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Norja

Hokynar

Aaltonen³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

253

216

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“…In addition, all antigens were tested with B19 acute-phase (low IgG avidity and acute epitope type specificity) and past-immunity (high IgG avidity and nonacute epitope type specificity) human serum control pools (29,61,62). Serum samples for IgG cross-reactivity study.…”

Section: B19 Virus (Types 1 To 3)-containing Samples and Hemagglutinamentioning

confidence: 99%

Biological and Immunological Relations among Human Parvovirus B19 Genotypes 1 to 3

Ekman

Hokynar

Kakkola

et al. 2007

J Virol

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The human parvovirus B19 is now divided into three genotypes: type 1 (prototype), type 2 (A6-and LaLi-like), and type 3 (V9-like). In overall DNA sequence, the three virus types differ by ϳ10%. The most striking DNA dissimilarity, of >20%, is observed within the p6 promoter region. Because of the scarcity of data on the biological activities and pathogenetic potentials of virus types 2 and 3, we examined the functional characteristics of these virus types. We found the activities of the three p6 promoters to be of equal strength and to be most active in B19-permissive cells. Virus type 2 capsid protein VP2, alone or together with VP1, was expressed with the baculovirus system and was shown to assemble into icosahedral parvovirus-like particles, which were reactive in the hemagglutination assay. Furthermore, sera containing DNA of any of the three B19 types were shown to hemagglutinate. The infectivities of these sera were examined in two B19-permissive cell lines. Reverse transcription-PCR revealed synthesis of spliced B19 mRNAs, and immunofluorescence verified the production of NS and VP proteins in the infected cells. All three genotypes showed similar functional characteristics in all experiments performed, showing that the three virus types indeed belong to the same species, i.e., human parvovirus B19. Additionally, the antibody activity in sera from B19 type 1-or type 2-infected subjects (long-term immunity) was examined with homo-and heterologous virus-like particles. Cross-reactivity of 100% was observed, indicating that the two B19 genotypes comprise a single serotype.Human parvovirus B19, a member of the genus Erythrovirus within the subfamily Parvovirinae, has long been considered the only human pathogen of its family, in which the adeno-associated viruses of the genus Dependovirus conceivably are apathogenic. However, new parvoviruses distinct from the genus Erythrovirus were recently detected in plasma (PARV4 and PARV5) (20, 28) and in nasopharyngeal aspirates (human bocavirus) (1), the last of which is supposedly associated with severe respiratory illness in small children.Although infection with parvovirus B19 typically results in erythema infectiosum or fifth disease (4), more severe or even lethal manifestations can occur among predisposed individuals. The virus replicates in erythroid progenitor cells of bone marrow (49, 64), causing aplastic crisis in patients with hemolytic anemia of various etiologies (2, 53, 56). During pregnancy, B19 can be transmitted from the infected mother to the fetus and cause fetal hydrops and death (9). In the immunocompromised, B19 infection may remain persistently productive, leading to chronic anemia (31).The B19 virus is small and nonenveloped and encapsidates a linear single-stranded DNA genome of ϳ5.6 kb. The two genomic ends contain identical inverted terminal repeats of ϳ380 nucleotides that are imperfect palindromes and form hairpin loops (13). The genome contains only one functional promoter, p6, located in the 3Ј palindrome (15). The p6 promoter regulates...

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“…In the secondary infection, the rapid antibody response is characterized by the production of high-avidity antibodies (23). The IgG antibody avidity test has been used to diagnose acute viral infections (5,12,17,21,27), to discriminate recent primary infection from reinfection or reactivation (1,2,4), to estimate the efficacy of vaccines (22), and to identify vaccine failures (18,19). The aim of the present study was to evaluate the IgG avidity test for differentiating primary from secondary dengue infections.…”

mentioning

confidence: 99%

Use of an Immunoglobulin G Avidity Test To Discriminate between Primary and Secondary Dengue Virus Infections

Souza

Fernandes

Araújo³

et al. 2004

J Clin Microbiol

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An enzyme-linked immunosorbent assay-based immunoglobulin G (IgG) antibody avidity test was evaluated by using sera from 57 patients with acute dengue infection. Overall, 55 of 57 patients were correctly classified (27 of 27 with primary dengue and 28 of 30 with secondary dengue). We conclude that the IgG avidity test can be useful for differentiating between acute, primary, and secondary dengue infections.

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Accurate Serodiagnosis Of B19 Parvovirus Infections By Measurement Of Igg Avidity

Cited by 87 publications

References 12 publications

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue

Biological and Immunological Relations among Human Parvovirus B19 Genotypes 1 to 3

Use of an Immunoglobulin G Avidity Test To Discriminate between Primary and Secondary Dengue Virus Infections

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