ACE inhibition and antioxidant activity of different part of <i>Channa striata</i> prepared by various cooking method

Channa striata or Snakehead fish is well-known as medicinal fish in Asian countries, including Indonesia. It is rich in functional amino acid as well as high protein. Previous study showed the fish extract was active as an ACE inhibitor in vitro, in which 5-10 kDa fraction of 50% ethanol extract has the potential ACE inhibitory activity (4,76% inhibition of ACE g-1 protein). This study aims to identify the native peptide in the Channa striata extract using LS-MS/MS. The 5-10 kDa extract fraction was further purified using Sephadex G-50, the significant peak fraction was treated with trypsin and untreated before being analysed using LC-MS/MS. The result showed that the untreated fraction had better result than trypsin treated one, indicating the significant peak fraction contains native peptide. We identified the presence of a 25.8 kDa β-actin fragment using the Channa striata database and two peptides GQVITIGNER and VITIGNER that meet the requirement software Proteome Discoverer 2.1. De Novo sequencing resulted 319 peptides with ALC >70%. Bioinformatics study revealed that the native peptide resulting from de novo sequencing was rich in inhibitor ACE peptides. These concluded that Channa striata contain a native peptide that is potentially an ACE inhibitor or anti-hypertension.

show abstract

Detection of native peptides from Channa striata extract using de novo sequencing

Chasanah

Martosuyono

Budiari

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

ACE inhibition and antioxidant activity of different part of Channa striata prepared by various cooking method

Cited by 1 publication

References 12 publications

Detection of native peptides from Channa striata extract using de novo sequencing

Detection of native peptides from Channa striata extract using de novo sequencing

Contact Info

Product

Resources

About