Acetaldehyde production by major oral microbes

Moritani, K; Takeshita, Toru; Shibata, Yukie; Ninomiya, Toshiharu; Kiyohara, Yutaka; Yamashita, Yoshihisa

doi:10.1111/odi.12341

Cited by 89 publications

(81 citation statements)

References 31 publications

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“…Neisseria species, present in the saliva of ≥80% of the subjects in this study, have been reported to produce considerable amounts of ACH from ethanol in vitro [9,13]. However, the salivary microbiota with less relative abundance of Neisseria species (type I community) showed significantly higher ACH production ability than that with the higher relative abundance of Neisseria species (type II community).…”

Section: Discussionmentioning

confidence: 79%

“…These findings highlighted that the salivary microbiota with higher relative abundance for Neisseria species independently associated with lower ACH production ability, although Neisseria are oral bacterial species that are known high-level ACH producers [9,13]. Furthermore, the association was independent of the total salivary bacterial count, alcohol intake frequency, and other important health characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…Many species of bacteria capable of producing relatively high amounts of ACH, such as S. salivarius, R. mucilaginosa , and Prevotella histicola [13], were observed in relatively higher proportions in the type I community, even though the relative abundance of Neisseria species was less. Our previous study confirmed that S. salivarius, R. mucilaginosa , and P. histicola are the predominant members of the salivary microbiota even in orally healthy subjects and are capable of producing relatively high amounts of ACH from ethanol in vitro [13]. Although these species do not produce as much ACH as Neisseria species, it is possible that their sheer abundance compensates for the absence of Neisseria and explains the high levels of ACH produced in subjects with the type I microbiota.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that Neisseria species are capable of producing extremely high amounts of ACH when cultured in vitro in a medium containing ethanol [9]. In addition, we previously analyzed ACH production ability by the prevalent bacteria in the salivary microbiota of orally healthy subjects and found that several bacterial species in the oral microbiota possessed the ability to produce ACH [13]. In particular, Neisseria species were confirmed to produce significant levels of ACH from ethanol in vitro , in agreement with the reported finding by Muto et al [9].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of oral microbiota and acetaldehyde production

Yokoyama

Takeuchi

Shibata

et al. 2018

Journal of Oral Microbiology

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Background: Neisseria has been reported to be a high producer of acetaldehyde (ACH), a carcinogen, from ethanol in vitro, but no information exists regarding whether the ACH production depends on oral microbiota profiles.Objective and Design: To explore the salivary microbiota profiles with respect to ACH production ability in the oral cavity using a cross-sectional design.Results: Using 16S rRNA gene amplicon sequencing, we classified 100 saliva samples into two types of communities (I and II). Salivary ACH production ability from ethanol was measured using gas chromatography and was found to vary over a 30-fold range. ACH production ability was significantly higher in the type I community, wherein the relative abundance of Neisseria species was significantly lower. Multivariate logistic regression analysis showed that the subjects with the type I community exhibited significantly higher probability of high ACH production ability than those with the type II community (P = 0.014). Moreover, the relative abundance of Neisseria species was inversely correlated with the ACH production ability (P = 0.002).Conclusion: The salivary microbiota profile with a lower relative abundance of Neisseria species was independently associated with high ACH production ability, despite Neisseria species are dominant producers of ACH in vitro.

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of oral microbiota and acetaldehyde production

Yokoyama

Takeuchi

Shibata

et al. 2018

Journal of Oral Microbiology

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“…The oral cavity is the first part of the digestive tract, which has contact with high-and low-concentrated alcohol. It can also be affected by ethanol metabolites [3]. Alcohol-based mouthwashes remain in longer contact with the oral cavity than alcoholic beverages when an alcoholic beverage is ingested.…”

Section: Introductionmentioning

confidence: 99%

Ethanol influence on gingival fibroblasts – a real-time in vitro study

Wyganowska‐Świątkowska

Nowak

Paszyńska

et al. 2018

Ann Agric Environ Med.

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Introduction. Alcohol consumption is the world's third largest risk factor for disease and disability. According to the WHO report from 2011: 71% of urban respondents ty and 77% of rural respondents admit to alcohol consumption]. Lower socio-economic status and educational levels result in a greater risk of alcohol-related injury, disease and death. Alcohol is a common component of many medicines, as well as an ingredient in many oral hygiene home products. Mouthwashes containing alcohol are considered to inhibit wound healing in the oral cavity. Due to the fact that many different results are described for different concentrations of alcohol at different times, an attemptwas made to visualise the direct impact of 7.2% and 22% alcohol on human gingival fibroblasts. Materials and method. PANsystem 2000 was used for visualisation of the reaction of human gingival fibroblasts isolated from gingiva on ethanol in 2 different concentrations. PANsys 3000 is a multi-system fully-automated cell culture device used for in vitro culture and to study a variety of cell lines under conditions similar to in vivo. Observations were carried out for 48 hours since alcohol addition. Pictures were taken in a continuous process at 5 minute intervalds and combined into a film. Results. Both contamination of 7.2% and 22% ethyl alcohol negatively affected morphology and cell proliferation. Addition of ethanol at a concentration 7.2% enabled cells to regain their ability to divide and recover normal morphology after 10 hours; changes caused by 22% ethanol, however, were irreversible. Conclusions. The obtained results suggest that daily usage of 7.2% alcohol contained in mouthwashes is non-toxic for gingival fibroblasts, and could be recommended after periodontal surgery.

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