Acetoacetate and ethyl acetoacetate as novel inhibitors of bacterial biofilm

Horne, Shelley M.; Schroeder, Meredith; Murphy, Jennifer; Prüβ, Birgit M.

doi:10.1111/lam.12852

Cited by 11 publications

(5 citation statements)

References 52 publications

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“…Potentially useful microorganisms are recommended for water cooling systems [22]. To prevent the formation of biofilms, the methods of the penetration of biocides into the matrix are promising, minimizing the primary contamination and adhesion of bacterial cells is recommended, for example, the effectiveness of acetoacetate and ethyl acetoacetate as inhibitors of Y. enterocolitica biofilm has been established [23]. Monoacylglycerols with two specific segments of the fatty acid residue, monolaurin, and monoepherin have strong inhibitory activity against bacterial biofilm Y. enterocolitica that is considered a promising method for use in the food industry [24].…”

Section: Discussionmentioning

confidence: 99%

Features of formation of Yersinia enterocolitica biofilms

et al. 2019

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Aim: The work aimed to study the morphology of colonies and their comparison by features of the formation of Yersinia enterocolitica biofilms. Materials and Methods: Bacteria were cultured on a Yersinia Selective Agar medium ("CIN-agar") at 28°C for 24 h. The microorganisms were grown in meat-peptone broth with 1.0% glucose to measure the absolute values of the optical density of the culture. The optical density of the liquid was determined in a microplate photometric analyzer Immunochem-2100 (HTI, USA) at a wavelength of 490 nm. For the study of biofilms, the specimens were fixed for 3-5 h in pairs of 25.0% solution of glutaraldehyde (according to DV), and pairs of a 1.0% aqueous solution of osmic acid (OSO4) were used for contrasting for 2-3 min. The specimens were examined with stereoscopic microscopy "BIOMED MS-1 Stereo" (Russia) and scanning electron microscope "TM 3030 plus" (Holland). Results: With stereoscopic microscopy of the colonies of Y. enterocolitica, the S-forms had an elevated intensely colored center, radial striation along the periphery, smooth edges, d ≤ 1.0 mm. R-form colonies had a dark color and a dry surface, were tuberous and had a dense center with a peripheral ridge, rugged edges, d ≥ 1.5 mm. The optical density of the Y. enterocolitica S-form showed that this type of microorganism belongs to the moderate producers of biofilms since the optical density of the sample (density of the sample - Ds) exceeded the optical density of control (density of the control - Dc) by 3 times. In Y. enterocolitica R-form (D ≤ 0.197) weakly produced biofilms, the optical density of the sample exceeded the optical density of the control by <2 times. Conclusion: The ability to form biofilms, the variability of phenotypic features, and the multiplicity of virulence factors of bacteria significantly reduce the effectiveness of diagnostic studies. The development of accelerated methods of detection and differentiation of the virulent properties of pathogenic bacteria will allow scientifically to substantiate and develop a set of measures aimed at preventing animal diseases and obtaining safe livestock products to prevent human diseases. Thus, we need to pay attention to which forms of colonies do Y. enterocolitica form on solid nutrient media: S- or R-forms. Through this study, we know that bacteria-forming S-shaped colonies are more capable of forming biofilms than R-forms. It means that they are more pathogenic and can cause persistent infections due to adhesion and biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Features of formation of Yersinia enterocolitica biofilms

et al. 2019

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“…In our study, the addition of 1% tomato homogenate to the 8% EAA wash did not reduce the efficacy of the wash, which is an advantage of EAA overusing chlorine. In addition, EAA was identified as an inhibitor of biofilm on polystyrene plates by three pathogens [ 10 ] and an inhibitor of biofilm in silicone tubing by several pathogens, including S. enterica [ 11 ]. This is the second advantage of EAA over chlorine that induced the formation of biofilm [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…An early study by our own research laboratory identified ß-phenylethylamine and acetoacetate as inhibitors of growth and biofilm in liquid beef broth growth medium and refrigeration temperature [ 9 ]. Ethyl acetoacetate (EAA) is chemically and structurally similar to acetoacetate but was more effective at reducing growth, live bacterial counts, and biofilm in three bacterial pathogens [ 10 ]. It is also more cost-effective.…”

Section: Introductionmentioning

confidence: 99%

A Wash of Ethyl Acetoacetate Reduces Externally Added Salmonella enterica on Tomatoes

Horne

Prüß

2022

Antibiotics

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The continuously high numbers of food-borne disease outbreaks document that current intervention techniques are not yet satisfactory. This study describes a novel wash for tomatoes that can be used as part of the food processing chain and is designed to prevent contamination with serovars of Salmonella enterica. The wash contains ethyl acetoacetate (EAA) at a concentration of 8% in H2O. This wash reduced live bacterial counts (on Salmonella Shigella agar) of externally added S. Newport MDD14 by 2.3 log, counts of S. Typhimurium ATCC19585 by 1.5 log, and counts of S. Typhimurium FSL R6-0020 by 3.4 log. The naturally occurring background flora of the tomatoes was determined on plate count agar. The log reduction by EAA was 2.1. To mimic organic matter in the wash, we added 1% tomato homogenate to the 8% EAA solution. Prior to using the wash, the tomato homogenate was incubated with the EAA for 2 h. In the presence of the tomato homogenate, the log reductions were 2.4 log for S. Newport MDD14 and 3 log for S. Typhimurium FSL R6-0020. It seems like tomato homogenate did not reduce the efficacy of the EAA wash in the two S. enterica serovars tested. We propose the use of EAA as a wash for tomatoes to reduce bacterial counts of S. enterica well as naturally occurring background flora.

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“…Ham and Kim (2016) reported that monoacylglycerols with two specific lengths of fatty acid moiety, monolaurin and monobehenin at 48 mg l −1 , were found to have strong inhibitory activity towards Y. enterocolitica biofilm formation, without a clear correlation between biofilm formation and cell growth. More recently, acetoacetate and ethyl acetoacetate were found to be effective in reducing growth and biofilm amounts with IC 50 values of 14·0 and 3·2 mg l −1 for planktonic growth, respectively, and 1 mg l −1 for biofilm biomass (Horne et al 2018). In all cases, authors have not been able to find compounds that act only on biofilm formation without inhibiting cell growth.…”

Section: Discussionmentioning

confidence: 99%

Aporphinoid alkaloids inhibit biofilm formation of Yersinia enterocolitica isolated from sausages

Marco

Pungitore

Estrada

2020

J. Appl. Microbiol.

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Aims of the study The ability of Yersinia enterocolitica strains to form biofilms and the capacity of different alkaloids to inhibit biofilm formation were investigated. Methods and Results The capacity to form biofilm on polystyrene of 31 Y. enterocolitica strains was evaluated. Biofilm and quorum sensing (QS) inhibition of 17 alkaloids were assayed; furthermore, minimum biofilm inhibitory concentration (MBIC) was determined. The capacity to form biofilms among the examined strains seemed to be a strain‐related feature. The best biofilm inhibitors at 100 µmol l−1 were oliverine (1), guatterine (3), liriodenine (4), oliveridine (5) and pachypodanthine (6), which showed biofilm inhibition higher than 87%. Pachypodanthine (6) was the most effective compound with MBIC value of 12·5 µmol l−1 at subinhibitory concentration and also was able to inhibit QS system and reduce yenR expression at this concentration. Conclusion This is the first study to demonstrate that oliverine, liriodenine, and pachypodanthine are able to inhibit biofilm formation of Y. enterocolitica without critically disturbing its growing capacity. At MBIC, pachypodanthine inhibited biofilm formation and QS. Significance and Impact of the Study The use of aporphinoid alkaloids as biofilms inhibitory agents might potentially be useful to treat biofilm‐associated infections in the future.

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Acetoacetate and ethyl acetoacetate as novel inhibitors of bacterial biofilm

Cited by 11 publications

References 52 publications

Features of formation of Yersinia enterocolitica biofilms

Features of formation of Yersinia enterocolitica biofilms

A Wash of Ethyl Acetoacetate Reduces Externally Added Salmonella enterica on Tomatoes

Aporphinoid alkaloids inhibit biofilm formation of Yersinia enterocolitica isolated from sausages

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