Acetogenins of Annona muricata leaves: Characterization and potential anticancer study

Daud, Nik Nurul Najihah Nik Mat; Yaakob, Harisun; Rosdi, Mohamad Norisham Mohamad

doi:10.15761/icst.1000202

Cited by 16 publications

(17 citation statements)

References 14 publications

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“…As an herbal plant, soursop leaves are consumed by the community as an antihypertensive agent [5], anti-diabetic [6], anti-inflammatory [7], anti-obesity [8], antioxidant [9], anti-hyperlipidemic [10], antirheumatic [11], antibacterial [12], antidepressant [13], and anticancer [14]. Soursop leaves contain active compounds, including acetogenin [15], alkaloids, terpenoids, flavonoids, coumarins, steroids, fatty acids, phlorotannins, phenolics, tannins, and saponins [16]. Although soursop leaves have been shown to have anti-obesity effects in multiple in vivo studies, the mechanism by which its active ingredients, individually or in combination, might act as anti-obesity agents have not been studied.…”

Section: Introductionmentioning

confidence: 99%

The Potency of Soursop Leaf Water Extract on Activating GLP-1r, Inhibiting Dpp4 and Foxo1 Protein Based on in Silico Analysis

et al. 2019

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Objective: Soursop leaf contains alkaloids, terpenoids, flavonoids, acetogenin, and phenolics. Soursop leaf decoction is usually consumed as an anti-obesity agent, even though its active compounds and its action mechanism are still unclear. Computational approaches have been developed to predict the ability of an active compound to bring about biological effects. This study was designed to predict the potency of soursop (Annona muricata Linn.) water extract for the activating of Glucagon-Like Peptide-1 Receptor (GLP-1R), Inhibiting Dipeptidyl Peptidase 4 (DPP4), and Forkhead Box Protein O1 (FoxO1) protein using in silico analysis. Methods: Identification of active compounds contained in soursop leaf water extract was carried out using physicochemical methods and Gas chromatography–mass spectrometry (GC-MS). They were then analyzed using computational analysis, i.e., potential analysis using the Way2drug web server. Protein interaction predictions of GLP-1R with the active compounds found in soursop leaf water extract using STITCH. The affinities of the active compounds of soursop leaf to the proteins DPP4 and FoxO1 were also analyzed using molecular docking. Results: Active compounds of the soursop leaf water extract contain 5-isopropenyl-3,8-dimethyl-1,2,3,3A, 4,5,6,7-octa hydro azulene (22.17%) and 1,2-benzene dicarboxylic acid, diethyl ester (phthalic acid) (57.30%). The active ingredients have not been shown to interact with GLP-1R. 5-isopropenyl-3,8-dimethyl-1,2,3,3A, 4,5,6,7-octa hydro azulene and phthalic acid both have a weak affinity for DPP4, and only phthalic acid has a weak affinity with the FoxO1 protein. Conclusion: Phthalic acid has a weak potential as an inhibitor of the DDP4 and FoxO1 proteins.

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Section: Introductionmentioning

confidence: 99%

The Potency of Soursop Leaf Water Extract on Activating GLP-1r, Inhibiting Dpp4 and Foxo1 Protein Based on in Silico Analysis

et al. 2019

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“…Even though there is no consensual method for preparing botanical extracts, the solvent used for soaking the plant material is often subsquently eliminated and the extract concentrated to dryness by low-pressure evaporation/rotary evaporation. Such a method offers the advantage of obtaining a weighable solid extract that can be easy stored and preserved (Cunha et al, 2017;Nik Mat Daud et al, 2016;Yang et al, 2015). While experimenting with methanolic and ethanolic extraction process, we dried the GLEs by rotary evaporation.…”

Section: Resultsmentioning

confidence: 99%

Antiproliferative Properties of Ethanolic and Aqueous Graviola Leaf Extracts on Tongue Squamous Cell Carcinoma-25.

Velázquez-Aponte

Cassé

2020

IJCRR

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Background: Annona muricata, commonly known as Graviola, soursop or guanabana, is an evergreen tree native to the tropics with a long history of use in ethnomedicine in indigenous communities in Africa and South America. Its active phytoconstituents have provided medicinal benefits against various ailments and diseases such as arthritis, parasitic infection, hypertension, fever, or diabetes. Studies conducted in vitro and in vivo have concluded that Graviola phytocomponents have anti-cancer and anti-tumor properties. One of the characteristics of cancer cells is their uncontrolled proliferation rate. In that sense, molecules that inhibit cell proliferation offer potential therapeutical benefits. Methods:We prepared ethanolic and aqueous extracts from dried Graviola leaves and tested their respective antiproliferative activities on tongue Squamous Cell Carcinoma cell line-25. We treated the cells with increasing concentrations of the extracts for 24 h. The respective doses leading to a 50% inhibition of cells growth (GI 50 ) were determined.Results: Our results showed that the ethanolic extract was 4 times more active in inhibiting the growth of Squamous Cell Carcinoma cell line-25 than the aqueous extract (respective GI 50 of 61.7 g/mL, and 274.6 g/mL). Conclusion:We hypothesize that some organic compounds involved in the antiproliferative/cytotoxicity of Graviola leaves were selectively extracted by Ethanol. Future plans include characterizing those bioactive compounds and assessing their activity on Squamous Cell Carcinoma cell line-25 vs. noncancerous oral cells. Our hope is to discover natural molecules to be used as alternative treatments for oral Squamous Cell Carcinomas.

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“…21 The screening involved the detection of alkaloids, terpenoids, flavonoids, coumarins, steroids, fatty acids, phenolics, tannins, phlobatannins, and saponin. The methods used in this study are summarized in Table 1 that was adapted from Nik Mat Daud et al 22…”

Section: Phytochemical Screening Of the Ame Extractsmentioning

confidence: 99%

In vitro studies of Annona muricata L. extract‐loaded electrospun scaffolds for localized treatment of breast cancer

et al. 2021

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This paper presents in vitro studies of the sustained release of Annona muricata leaf extracts (AME) from hybrid electrospun fibers for breast cancer treatment.Electrospun hybrid scaffolds were fabricated from crude AME extracts, poly(lacticco-glycolic acid)/gelatin (PLGA/Ge) and pluronic F127. The physicochemical properties of the AME extract and scaffolds were studied. The antiproliferative effects of the scaffolds were also assessed on breast cancer (MCF-7 and MDA-MB-231) and non-tumorigenic breast (MCF10A) cell lines. Scanning electron microscope micrographs revealed a random network of micro-and submicron fibers. In vitro drug release profiles, governed by quasi-Fickian diffusion at pH 7.

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Acetogenins of Annona muricata leaves: Characterization and potential anticancer study

Cited by 16 publications

References 14 publications

The Potency of Soursop Leaf Water Extract on Activating GLP-1r, Inhibiting Dpp4 and Foxo1 Protein Based on in Silico Analysis

The Potency of Soursop Leaf Water Extract on Activating GLP-1r, Inhibiting Dpp4 and Foxo1 Protein Based on in Silico Analysis

Antiproliferative Properties of Ethanolic and Aqueous Graviola Leaf Extracts on Tongue Squamous Cell Carcinoma-25.

In vitro studies of Annona muricata L. extract‐loaded electrospun scaffolds for localized treatment of breast cancer

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