Acetyl Salicylic Acid Locally Enhances Functional Recovery After Sciatic Nerve Transection in Rat

Abstract

Local effect of acetyl salicylic acid (ASA) on peripheral nerve regeneration was studied using a rat sciatic nerve transection model. Forty-five male healthy White Wistar rats were divided into three experimental groups (n = 15), randomly: Sham-operation (SHAM), control (SIL), and ASA-treated (SIL/ASA) groups. In SHAM group after anesthesia left sciatic nerve was exposed through a gluteal muscle incision and after homeostasis the muscle was sutured. In SIL group the left sciatic nerve was exposed the same way and transected proximal to tibio-peroneal bifurcation leaving a 10-mm gap. Proximal and distal stumps were each inserted into a silicone tube and filled with 10 μl phosphate buffered solution. In SIL/ASA group defect was bridged using a silicone tube filled with 10 μl acetyl salisylic acid (0.1 mg/ml). Each group was subdivided into three subgroups of five animals each and were studied 4, 8, and 12 weeks after surgery. Data were analyzed statistically by factorial analysis of variance (ANOVA) and the Bonferroni test for pair-wise comparisons. Functional study confirmed faster and better recovery of regenerated axons in SIL/ASA than in SIL group (p < 0.05). Gastrocnemius muscle mass in SIL/ASA was significantly more than in SIL group. Morphometric indices of regenerated fibers showed that the number and diameter of the myelinated fibers in SIL/ASA were significantly higher than in control group. In immuohistochemistry, location of reactions to S-100 in SIL/ASA was clearly more positive than in SIL group. Response to local treatment of ASA demonstrates that it influences and improves functional recovery of peripheral nerve regeneration.

Schwann cell‑like cells derived from human amniotic mesenchymal stem cells promote sciatic nerve repair through an exosome‑induced SOX2/FN1 pathway in vitro

“…Clements et al (46) reported that chitosan fibers displayed 90% success in bridging a 12-mm gap in the sciatic nerve after 1 month. Mohammadi et al (47) reported that silicone conduit neurorrhaphy promoted nerve regeneration at the time of sciatic nerve repair. In the present study, NT-3 chitosan conduits were filled with iSC hydrogel to promote the repair of damaged sciatic nerve.…”

Effects of alpha lipoic acid on motor function and antioxidant enzyme activity of nerve tissue after sciatic nerve crush injury in rats

“…There are a lot of studies evaluating the protective or therapeutic effects of various antioxidants related to the peripheral nerve injury model such as vitamin E, ubiquinone, L-carnitine and agmatine (1,22,33,38) or other substances such as cytidinediphosphocholine, rapamycin, tetanus toxin, platelet-rich plasma, insulin-like growth factor I, dexamethasone and acetyl salicylic acid (5,8,9,12,17,23,29,39), or some energy types such as low level laser irradiation and low-frequency magnetic fields (30,44).…”

“…Therefore, we decided to evaluate the functional status of the subjects by SFI in addition to nerve tissue levels of antioxidants. A rat model was chosen because the rat sciatic nerve can be easily dissected and it is a well-studied model in literature (2,5,9,12,17,29,31,(37)(38)(39)44).…”

Estudio comparativo de la reparación del sistema nervioso periférico con técnicas microquirúrgicas experimentales de sutura epineural en condiciones de distintos grados de tensión

“…77 Actualmente se encuentran en fase experimental estudios de bioingeniería en los cuales se colocan sustancias dentro del neurotubo, que contienen factores que estimulan el crecimiento axonal y medios de soporte para el crecimiento de estos últimos, como cultivos de células de Schwann 78 , factor de crecimiento nervioso, ácido acetilsalicílico y factor neurotrófico ciliar por solo citar algunos ejemplos. 4,42,79…”

“…A pesar de que no fueron utilizados antibióticos como profilaxis prequirúrgica no se registraron infecciones de las heridas, hecho ya reportado por otros autores en la literatura 84,100,101. Tampoco evidenciamos contracturas crónicas de los miembros abordados, producidas por anquilosis articulares o por reinervación fallida, producida por la pérdida de la ruta original de los axones, y que involucra a músculos agonistas y antagonistas a la vez.…”