Acetylcholine increases intracellular Ca<sup>2+</sup>in the rat pituitary folliculostellate cells in primary culture

Nakajima, Yusaku; Uchiyama, Maki; Shirai, Yoshio; Sakuma, Yasuo; Kato, Masakatsu

doi:10.1152/ajpendo.2001.280.4.e608

Cited by 7 publications

(9 citation statements)

References 21 publications

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“…It causes a dose-dependent release of Anx A1 from TtT/GF cells. Acetylcholine acts on cultured primary rat F-S cells via muscarinic receptors to stimulate calcium transients by causing release from intracellular stores [28]. However, our TtT/GF cell cultures fail to generate calcium transients to even larger doses of acetylcholine, although they readily respond in this way to ATP (Morris, Williams, Christian, unpublished observations).…”

Section: Inputs To F-s Cells: Receptors and Mecha-nisms Of Actionmentioning

confidence: 89%

“…While the availability of these cell lines has been most useful, there is often the implicit assumption that these cell lines entirely represent the native F-S cells. However, TtT/GF cells lack nNOS [27] and we have recently found that, unlike native rat cultured F-S cells [28], TtT/GF cells do not respond to acetylcholine by a rise in intracellular calcium ([Ca] i ) although they can respond to acetylcholine by exporting Anx A1 [29]. The first human F-S cell line (PDFS) was derived spontaneously from a clinically non-functioning pituitary macroadenoma [30].…”

Section: F-s Cell Linesmentioning

confidence: 99%

“…Rat pituitary F-S cells [28] Choline (implied) TtT/GF cells [29] Bradykinin (implied) Rat pituitary F-S cells [72] Angiotensin-II (AT-1; implied)…”

Section: Communication With the Hypothalamo-pituitary Adrenal Stress mentioning

confidence: 99%

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Folliculo-stellate Cells: Paracrine Communicators in the Anterior Pituitary

Morris¹

2011

TONEUROEJ

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Most research on the anterior pituitary (adenohypophysis) has concentrated on the endocrine cells characterized by their complement of cytoplasmic dense-cored vesicles containing the classic anterior pituitary hormones. However it has become increasingly clear over the last 20 years that cells first identified more than 50 years ago in the basis that they lack such dense-cored vesicles and now known generically as folliculo-stellate or follicular cells have important physiological functions and act as an adenohypophysis wide communication system. This brief review reveals the need for this communication system, what we know of the plethora of products secreted by Folliculo-Stellate cells, the many receptors to which they respond, and in particular, the role of these enigmatic cells in the physiology of the stress/immune axis, the gonadotroph cells and the pituitary vasculature. Finally we review the current evidence that cells in this category can act as stem cells in the adult pituitary.

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Section: Inputs To F-s Cells: Receptors and Mecha-nisms Of Actionmentioning

confidence: 89%

Section: F-s Cell Linesmentioning

confidence: 99%

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Folliculo-stellate Cells: Paracrine Communicators in the Anterior Pituitary

Morris¹

2011

TONEUROEJ

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“…Carbachol inhibition of GH release in aggregates can be blocked by the calcium channel blockers cadmium and verapamil (594), consistent with the activation of nNOS by intracellular free calcium (3, 598). FS cells express muscarinic receptors, probably of the M1 type, that mediate activation of phospholipase C and intracellular free Ca 2+ rises (4, 599). Carbachol does not inhibit GH secretion in the GH3 cell line cultured as aggregates (5, 594).…”

Section: Corticotrophs As Autocrine/paracrine Cells and Targetsmentioning

confidence: 99%

“…FS cells express β1 and β2-adrenergic receptors (720), acetylcholine (599), VIP and PACAP receptors (721), angiotensin II receptor-1 (722), adenosine A1 and A2B receptors (723) and the TSH receptor (724). Ligands of some of these receptors are known to modulate the function of immune cells.…”

Section: Paracrine Control By Nonhormonal Cellsmentioning

confidence: 99%

Endogenous Cannabinoids: Structure and Metabolism

Bisogno

2008

J Neuroendocrinology

144

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D9 -Tetrahydrocannabinol (THC), the main psychoactive compound extracted from Cannabis sativa, is the most representative component of the mixture of therapeutic substances that are communally referred to as cannabinoids (1). It acts as an agonist at specific G-protein-coupled receptors, cannabinoid receptor subtypes CB 1 and CB 2 (2). The discovery of cannabinoid receptors has driven the investigation of the existence of their natural ligands and, soon after, endocannabinoids were identified. N-arachidonoyl-ethanolamine (AEA, anandamide) and 2-arachidonoyl glycerol (2-AG) are the two most studied endocannabinoids. They are biosynthesised 'on demand' by cleavage of their membrane lipid precursors N-arachidonoyl-phosphatidylethanolamine (N-ArPE) and sn-1-acyl-2-arachidonoylglycerols (DAGs) respectively, and then inactivated by intracellular hydrolysing enzymes. Cannabinoid receptors, endocannabinoids and enzymes that biosynthesise [N-acyl-phosphatidylethanolamine-selective phospholipase D (NAPE-PLD) and diacylglycerol lipase (DAGL)] and degrade [fatty acid amide hydrolase (FAAH) and the monoacylglycerol lipases (MAGL)] AEA and 2-AG, respectively, are key components in the endocannabinoid system (3). Despite a large number of excellent studies having contributed to the current understanding of the endocannabinoid regulation and function, further efforts are necessary to fully comprehend this signalling system. Here, a review on the current knowledge on structure and metabolism of the best studied endogenous cannabinoids is presented. In particular, this review concentrates on the mechanisms responsible for the biosynthesis and inactivation of the endocannabinoids, as well as on the several inhibitors of their metabolism identified to date. Endocannabinoids and related N-acyl-ethanolaminesEndocannabinoids are defined as endogenous compounds capable of binding to and functionally activating cannabinoid receptors. At least five endocannabinoids have been identified to date (Fig. 1). Anandamide, the amide between arachidonic acid and ethanolamine, was the first endogenous ligand to be reported at the end of 1992 (4) and additional polyunsaturated ethanolamines that bind to cannabinoid receptors, homolinolenylethanolamide and docosatetraenylethanolamide, have been isolated in the brain. Other endocannabinoids, all derived from arachidonic acid, were identified. The identification of 2-AG, the ester of arachidonic acid with glycerol, isolated from canine gut and brain has also been reported (5, 6). Subsequently, the first endocannabinoid characterised by ether bond, 2-arachidonyl glyceryl ether or noladin ether, was isolated from porcine brain (7) and N-arachidonoyldopamine (NADA), a selective CB 1 agonist and a potent agonist of vanilloid receptors, was discovered (8, 9). O-arachidonoylethanolamine, the ester derivative of ethanolamine with arachidonic acid, with the same molecular weight as anandamide but with opposite orientation of the polar ethanolamine moiety, was named virodhamine from the Sanskrit word virodha, ...

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Neurotransmitter receptors as signaling platforms in anterior pituitary cells

Zemková

Stojilković

2018

Molecular and Cellular Endocrinology

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The functions of anterior pituitary cells are controlled by two major groups of hypothalamic and intrapituitary ligands: one exclusively acts on G protein-coupled receptors and the other activates both G protein-coupled receptors and ligand-gated receptor channels. The second group of ligands operates as neurotransmitters in neuronal cells and their receptors are termed as neurotransmitter receptors. Most information about pituitary neurotransmitter receptors was obtained from secretory studies, RT-PCR analyses of mRNA expression and immunohistochemical and biochemical analyses, all of which were performed using a mixed population of pituitary cells. However, recent electrophysiological and imaging experiments have characterized γ-aminobutyric acid-, acetylcholine-, and ATP-activated receptors and channels in single pituitary cell types, expanding this picture and revealing surprising differences in their expression between subtypes of secretory cells and between native and immortalized pituitary cells. The main focus of this review is on the electrophysiological and pharmacological properties of these receptors and their roles in calcium signaling and calcium-controlled hormone secretion.

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Acetylcholine increases intracellular Ca²⁺in the rat pituitary folliculostellate cells in primary culture

Cited by 7 publications

References 21 publications

Folliculo-stellate Cells: Paracrine Communicators in the Anterior Pituitary

Folliculo-stellate Cells: Paracrine Communicators in the Anterior Pituitary

Endogenous Cannabinoids: Structure and Metabolism

Neurotransmitter receptors as signaling platforms in anterior pituitary cells

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Acetylcholine increases intracellular Ca2+in the rat pituitary folliculostellate cells in primary culture

Cited by 7 publications

References 21 publications

Folliculo-stellate Cells: Paracrine Communicators in the Anterior Pituitary

Folliculo-stellate Cells: Paracrine Communicators in the Anterior Pituitary

Endogenous Cannabinoids: Structure and Metabolism

Neurotransmitter receptors as signaling platforms in anterior pituitary cells

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Acetylcholine increases intracellular Ca²⁺in the rat pituitary folliculostellate cells in primary culture