Acetylcholine iontophoresis in diabetic patients with and without peripheral neuropathy: a potential therapeutic tool

Zaid, Ibrahim Ismail Abu; Hussein, Mohamed A.; Mousa, Gihan Samir; Mohamed, Amany R.; Badr, Nagwa M.; Hamid, Alaa A.

doi:10.4103/ejim.ejim_51_17

Cited by 1 publication

(1 citation statement)

References 23 publications

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“…The amount of permeated drug was examined by ELISA. 17) In Vitro Permeation Study by Franz Diffusion Cell under Iontophoresis The Franz cell was prepared as described in passive diffusion and then the iontophoretic device was used to supply an electrical current to the intestinal membrane fixed between the two compartments. The cathode was connected to the donor chamber and the anode was connected to the receptor chamber with a distance of 1 cm away from the membrane.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and in Vivo Comparative Study of Oral Nanoparticles and Gut Iontophoresis as Oral Delivery Systems for Insulin

ELKHATIB

Ali²,

Al-badrawy³

2021

Biological & Pharmaceutical Bulletin

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Multiple daily injections of insulin for diabetes cause many hazards for diabetic patients. Oral noninvasive insulin delivery could be more convenient and less painful than parenteral route. In past decades transdermal iontophoresis had been studied for insulin delivery across the skin with or without chemical permeation enhancers. However, the results of these studies were not efficacious and serum insulin levels were not therapeutically effective. In the present study an advanced technology "gut iontophoresis" for insulin delivery across the gut wall was compared with traditional oral insulin delivery in the form of nanoparticles. In vitro application of electric current to the intestinal membrane could enhance the flux of insulin nanoparticles (3.4 fold enhancement of insulin transport) from the donor to the receptor compartment in the Franz cell. In vivo iontophoresis of insulin nanoparticles through the gut wall would produce intense hypoglycemia (57% glycemia drop in 3 h) without damage of the intestinal tissues. Cell viability assay indicated that 50-500 µg/mL nanoparticles had no toxic effect on Caco-2 cells. Nanoparticles gut iontophoresis could be a promising non-invasive technique for oral insulin delivery.

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Section: Methodsmentioning

confidence: 99%