Acetylcholine Noise: Analysis after Chemical Modification of Receptor

Landau, Emmanuel M.; Ben-Haim, D.

doi:10.1126/science.185.4155.944

Cited by 33 publications

(12 citation statements)

References 9 publications

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“…These experiments were similar to, and their results in good agreement with, those previously reported (Katz & Miledi, 1970, 1971, 1972Landau & Ben-Haim, 1974;Ben-Haim, Dreyer & Peper, 1975). The grand average value of 138 estimates of the control ACh shot effect amplitude "a" drawn from 40 experiments was 0.31 + 0.01 gV (mean_+sE) (calculated according to the equations of Katz and Miledi, 1972, and employing their correction factor for nonlinear summation of depolarization).…”

Section: A Ch-induced Noisesupporting

confidence: 85%

Voltage fluctuations at the frog sartorius motor endplate produced by a covalently attached activator

Cox¹,

Kawai²,

Karlin³

et al. 1979

J. Membrain Biol.

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The depolarization that develops after covalent attachment of trimethylammonium benzoyl to the dithiothreitol-reduced frog sartorius acetylcholine receptor is accompanied by a small increase in voltage fluctuations. The amplitude of the elementary voltage event produced by the covalently attached activator is about 0.04 microV, almost an order of magnitude below the acetylcholine shot-effect amplitude in the control preparation, and about one-fourth the acetylcholine shot amplitude after disulfide-bond reduction. Spectral density plots of trimethylammonium-benzoyl noise can be analyzed in terms of two relaxation rates that bracket the single rate observed in response to acetylcholine.

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Section: A Ch-induced Noisesupporting

confidence: 85%

Voltage fluctuations at the frog sartorius motor endplate produced by a covalently attached activator

Cox¹,

Kawai²,

Karlin³

et al. 1979

J. Membrain Biol.

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“…Although a much larger dose of ACh is now required to produce a given depolarization, the associated noise remains the same, indicating that size and time course of the individual molecular actions are unchanged. Other substances, however, such as atropine, dithiothreitol and certain anaesthetics (Katz & Miledi, 1973a;Landau & Ben-Haim, 1974) greatly reduce the noise which accompanies an ACh-induced potential change and also shorten the time course of the underlying elementary event. These substances -unlike competitive blocking agents -modify the kinetics of the molecular ACh action, which suggests an attachment to an adjacent site rather than to the initial binding site of ACh itself (see also Lapa, Albuquerque & Daly, 1974).…”

Section: Introductionmentioning

confidence: 99%

The effect of procaine on the action of acetylcholine at the neuromuscular junction.

Katz¹,

Miledi²

1975

The Journal of Physiology

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SUMMARY1. The effect of procaine on acetylcholine-induced membrane fluctuations (ACh noise) was studied by intracellular and focal external recording from end-plates of frog sartorius muscle.2. With intracellular recording, ACh noise (the ratio of variance to mean depolarization) was substantially reduced by procaine, suggesting a greatly decreased amplitude of the elementary potential change.3. Spectral analysis of the ACh noise indicated a dual average time course of the underlying 'shot effects', similar to the complex shape (brief spike followed by a long tail of low intensity) of the end-plate current in procaine-treated muscle.4. The post-synaptic blocking action of procaine can be largely explained by the drastic shortening of the initial high-intensity phase of the end-plate conductance change.5. The average time course of the ACh shot effects is discussed in terms of alternative hypotheses, one attributing the complex shape to each elementary event, the other involving sequential reaction steps which produce two unequal populations of ion gates of different 'life-times'.

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“…Since the discovery of acetylcholine (ACh) 'noise' at the frog end-plate (Katz & Miledi, 1970), fluctuation analysis has become a powerful tool in the investigation of ACh action at the molecular level (Katz & Miledi, 1972, 1973a, b, 1975Anderson & Stevens, 1973;Landau & Ben-Haim, 1974; Ben-Haim, Dreyer & Peper, 1975;Colquhoun, Dionne, Steinbach & Stevens, 1975;Neher & Sakmann, 1976a, b;Dreyer Walther & Peper, 1976 We are interested in the properties of ACh receptors that appear early in myogenesis. Muscle cell cultures offer certain advantages for developmental studies.…”

Section: Introductionmentioning

confidence: 99%

Acetylcholine noise in cultured chick myoballs: a voltage clamp analysis.

Fischbach¹,

Lass²

1978

The Journal of Physiology

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SUMMARY1. Large spherical muscle cells were grown in vitro by allowing dissociated chick myoblasts to fuse in suspension culture.2. Myoball membranes could be adequately voltage clamped even in the face of large inward currents (ca. 100 nA) induced by ACh.3. The single channel conductance, y, estimated from the ratio of the mean to the variance of ACh currents was 25-40 pmho at temperatures between 250 and 37 0C. 4. Spectra of ACh current fluctuations declined with 1/f 2. The mean channel open time (7r) estimated from the half-power frequency was 2 msec at 37 0C and -90 mV holding potential. At lower membrane potentials 7 was decreased and on hyperpolarization r was prolonged.

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Acetylcholine Noise: Analysis after Chemical Modification of Receptor

Cited by 33 publications

References 9 publications

Voltage fluctuations at the frog sartorius motor endplate produced by a covalently attached activator

Voltage fluctuations at the frog sartorius motor endplate produced by a covalently attached activator

The effect of procaine on the action of acetylcholine at the neuromuscular junction.

Acetylcholine noise in cultured chick myoballs: a voltage clamp analysis.

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