Acetylcholinesterase (ACHE) and α-Glucosidase Inhibitory Assay by Effect-Directed Analysis on High Performance Thin-Layer Chromatography Coupled to Mass Spectrometry

Aranda, Mario; Carrasco, Jonathan; Henríquez, Karem

doi:10.1007/978-1-0716-2107-3_16

Cited by 3 publications

(3 citation statements)

References 11 publications

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“…At. Spectrom., 2023, 38,[1943][1944][1945][1946][1947][1948][1949][1950][1951] This journal is © The Royal Society of Chemistry 2023 microextraction extraction methods that evaluated uranium isotopes. 32 The isotope ratio values are naturally expected to be less than the theoretical values because the ICP-MS favorably transmits heavier isotopes through the interface and ion lenses.…”

Section: Resultsmentioning

confidence: 99%

“…32 The approach was based on the use of the Advion (Ithaca, NY) plate express as a means of solutionmicroextraction from a cloth substrate and transporting the eluate rst to a liquid sampling-atmospheric pressure glow discharge (LS-APGD) microplasma ionization source, 32 and more recently to ICP sources, [33][34][35][36] for MS detection. The device was originally designed as a means of extracting organic species from thin layer chromatography (TLC) plates, 37,38 for their transport to electrospray ionization (ESI) sources for qualitative analysis. In this approach, cotton swipes are placed underneath a capillary sampling head, wherein the elution solvent (2% HNO 3 ) passes across the surface of the substrate, dissolving/ desorbing (the exact process is not clear at this point) immobilized analyte and carrying those species to the respective ionization source.…”

Section: Introductionmentioning

confidence: 99%

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Direct, multielement determinations from cotton swipes via plate express microextraction coupled to an inductively coupled plasma mass spectrometer (μEx-ICP-MS)

Stouffer,

Marcus

2023

J. Anal. At. Spectrom.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Direct, multielement determinations from cotton swipes via plate express microextraction coupled to an inductively coupled plasma mass spectrometer (μEx-ICP-MS)

Stouffer,

Marcus

2023

J. Anal. At. Spectrom.

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“…⍺-glucosidase-inhibiting peptides were detected applying the protocol reported by Galarce-Bustos et al, [42] with slight modifications Aranda et al, [43]. Briefly, the plate was immersed into 100 mM phosphate buffer, pH 7.4, and dried on a plate heater for 10 min at 115 °C.…”

Section: Hptlc-bioassay: ⍺-Glucosidase Inhibitionmentioning

confidence: 99%

Multivariate optimization of microwave-assisted enzyme digestion of α-casein for generation of bioactive peptides

López,

Espinoza-Bello,

Carrasco

et al. 2023

J. Chil. Chem. Soc.

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Caseins are the principal milk proteins and serve as an important source of bioactive peptides with diverse beneficial effects for human health, such as antihypertensive, immunomodulating, anti-inflammatory, and antithrombotic. The objective of this study was to optimize, using the design of the experiment, the production of bioactive peptides from α-casein applying microwave-assisted enzyme digestion (MAED). The optimal MAED conditions (time, temperature, and enzyme/protein ratio) were established for pepsin (digestion time 4 min, temperature 41°C, and E:P ratio 1:40) and trypsin (digestion time 10 min, temperature 37°C and E:P ratio 1:200.) enzymes. Digestion yields and the intensity of different bioactivities, i.e., antimicrobial, antioxidant, and acetylcholinesterase and αglucosidase inhibitory activities, evaluated by the HPTLC-bioassay technique were used as response variables. Trypsin-formed α-casein peptides showed antimicrobial, antioxidant, and acetylcholinesterase inhibition activities, while pepsin-formed showed antimicrobial, antioxidant, and α-glucosidase inhibition activities. These results demonstrate that MAED is a fast and effective technique for bioactive peptide production from casein proteins.

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Exploring strategies to growth wild turnip sprouts as healthy food

López-Belchí,

Toro,

Illanes

et al. 2024

Chem. Biol. Technol. Agric.

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Background Brassicas (Brassicaceae) are recognized as excellent sources of nutrients and bioactive compounds. Among these, wild turnip (Brassica rapa L.), holds significant promising nutritional properties owed to its abundant glucosinolates and phenolic compounds. To enhance its potential values, the application of elicitors is crucial and good strategy prompting an enrichment in the concentration of phytochemicals, as well established in other relevant Brassicas, such as broccoli. While the responses triggered by certain elicitors such as salicylic acid, methyl jasmonate, or chitosan are widely documented, little is known about the impact of electrolyzed water, an economically viable elicitor. Through elicitation strategies, the aim of this work was to unravel insights into enhancing the phytochemical content of wild turnip sprouts for potential use as healthy food, comparing with well-studied broccoli as control of the experiments. Results Our findings revealed that wild turnip exhibited a notable higher glucosinolate (GSL) contents (487–712 mg 100 g−1 D.W.), than in broccoli sprouts. Furthermore, the use of electrolyzed water (2 vol.) boosted the accumulation of glucosinolates with significant increase up to twofolds the content. Specifically, treatments with salicylic acid (250 μM) and electrolyzed water (2 vol.) favored the significant increase of mainly aliphatic GSL (progoitrin, PRO; gluconapin, GNA; glucobrassicin, GBN). On the other hand, natural antioxidants such as of the characteristic acylated cyanidins present in wild turnip sprouts were not affected by the elicitor treatments, indicative of higher tolerance to oxidative stress in wild turnip. Conclusions These observations underlined the potential of using electrolyzed water in wild turnips as elicitor for GSL-enriched food ingredients. Further studies will be necessary to align with the broader goal of evaluating abiotic and biotic factors affecting the phytochemical composition in mature organs not only in germinating seeds and sprouts, for agricultural performance for quality and healthy foods purposes. Graphical Abstract

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Acetylcholinesterase (ACHE) and α-Glucosidase Inhibitory Assay by Effect-Directed Analysis on High Performance Thin-Layer Chromatography Coupled to Mass Spectrometry

Cited by 3 publications

References 11 publications

Direct, multielement determinations from cotton swipes via plate express microextraction coupled to an inductively coupled plasma mass spectrometer (μEx-ICP-MS)

Direct, multielement determinations from cotton swipes via plate express microextraction coupled to an inductively coupled plasma mass spectrometer (μEx-ICP-MS)

Multivariate optimization of microwave-assisted enzyme digestion of α-casein for generation of bioactive peptides

Exploring strategies to growth wild turnip sprouts as healthy food

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Acetylcholinesterase (ACHE) and α-Glucosidase Inhibitory Assay by Effect-Directed Analysis on High Performance Thin-Layer Chromatography Coupled to Mass Spectrometry

Cited by 3 publications

References 11 publications

Direct, multielement determinations from cotton swipes via plate express microextraction coupled to an inductively coupled plasma mass spectrometer (μEx-ICP-MS)

Direct, multielement determinations from cotton swipes via plate express microextraction coupled to an inductively coupled plasma mass spectrometer (μEx-ICP-MS)

Multivariate optimization of microwave-assisted enzyme digestion of &#945;-casein for generation of bioactive peptides

Exploring strategies to growth wild turnip sprouts as healthy food

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Multivariate optimization of microwave-assisted enzyme digestion of α-casein for generation of bioactive peptides