Acetylsalicylic acid decreases the labeling of blood constituents with technetium-99m

Abstract

Acetylsalicylic acid is the most widely used drug as antipyretic, analgesic, anti-inflammatory agent and for secondary prevention of thrombotic phenomena in the heart, brain and peripheral circulation. Drugs can modify the labeling of blood constituents with technetium-99m (99mTc). This work has evaluated the effect of in vivo treatment with acetylsalicylic acid on the in vitro labeling of the blood constituents with 99mTc. Wistar rats were treated with different doses (1.5, 3.0 and 6.0 mg/kg) of acetylsalicylic acid during 1 hour. At higher dose used (6.0 mg/kg) animals were treated during different period of time (0.25, 1.0 and 4.0 hours). Animals treated with physiologic saline solution were used as control. After the labeled process; plasma (P), blood cells (BC), insoluble (IF-P, IF-BC) and soluble (SF-P, SF-BC) fractions were separated. Afterwards, the percentage of radioactivity (%ATI) in each fraction was calculated. The treatment during 1 hour with acetylsalicylic acid at higher dose has significantly (p < 0.05) modified the fixation of 99mTc on blood cells. Considering the results, we suggest that acetylsalicylic acid used at therapeutic doses may interfere with the nuclear medicine procedures related to these blood constituents.

Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

“…Blood constituents labeled with 99mTc have been used in several clinical examinations (Saha, 2004) and also as an experimental assay on an attempt to verify the effect of drugs ). This experimental model has permitted to obtain relevant information about properties of various chemical compounds (synthetic and natural) (Abreu et al, 2006;Fonseca et al, 2007). The analysis of the results shown in tables 1, 2 and 3 indicates that there was an important alteration on the labeling of the blood constituents with 99m…”

“…Red blood cells labeled with technetium-99m (99mTc-RBC) are widely used in clinical nuclear medicine for several important applications (Wong et al, 2004;Jin et al, 2004;Zaman et al, 2004;Artiko et al, 2004;Harel et al, 2005;Verdu et al, 2005;Olds et al, 2005). The labeling of RBC with 99mTc has been used as assay to evaluate the properties of different chemical agents (Abreu et al, 2006;Fonseca et al, 2007). The radiolabeling depends on the presence of a reducing agent and stannous chloride is widely utilized.…”

Effect of an extract of Artemisia vulgaris L. (Mugwort) on the in vitro labeling of red blood cells and plasma proteins with technetium-99m

“…Some authors have been reported that natural and synthetic drugs could alter the labeling of blood constituents with 99mTc (Frydman et al, 2004;Abreu et al, 2006;Fonseca et al, 2007). The labeling of blood constituents would decrease due to the action of drugs that could (1) bind at the same sites of 99mTc on the blood constituents, (2) directly inhibit (chelating action) of the stannous and pertechnetate ions, (3) oxidize or generate free radicals that could oxidize the stannous ion, and (4) alter the plasma membrane structure or modify the transport systems of stannous and pertechnetate ions into cells.…”

Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents

“…Red blood cells labeled with technetium-99m ( 99m Tc) are also employed in diagnostic procedures (Saha, 2004). Drugs (natural and synthetic) could alter the biodistribution of radiopharmaceuticals and the labeling of blood constituents with 99m Tc (Bernardo-Filho et al, 2005;Fonseca et al, 2007. This study evaluates biological effects of a CA extract on the biodistribution of the radiopharmaceutical Na 99m TcO 4 and on the fixation of the radioactivity on blood constituents.…”